Expression of Pisum Sativum recombinant receptor proteins Sym10 and Sym37 involved in perception of lipochitooligosaccharide nod factors
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In the legume Rhizobium symbiosis, the Nod factors excreted by rhizobia trigger a complex of specific responses in the plant epidermis, pericycle, and root cortex, thereby providing a basis for subsequent bacterial entry and root nodule organogenesis. Nod factors are biologically active at low concentrations, and the specificity of their responses depends on the structural peculiarities of these molecules, suggesting the presence of high affinity receptors to Nod factors. Genetic analysis of pea mutants identified the genes necessary for symbiosis development and, among then, PsSym10 and PsSym37 encoding LysM-receptor-like kinases with LysM motifs (LysM-RLK) in the extracellular domain. These proteins are supposed to be potential receptors to Nod factors. However, there is no experimental evidence of the binding of Nod factors to the revealed LysM-RLK, which must confirm the biochemical function of receptors. For the most part, this is due to the problems with isolation of membrane receptors. In this work, heterologous expression of proteins SYM10 and SYM37 in bacterial cells was performed for the first time, the conditions for purification of recombinant proteins were optimized, and specific antibodies were obtained for further enzyme immunoassay of the two LysM-RLKs in legumes.
KeywordsPisum sativum L. Nod factors nodulation LysM receptors heterologous expression
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