Abstract
The goal of this work was to evaluate the efficiency of mast cell identification by different histochemical and immunohistochemical methods and to determine the features of their localization in the human pineal gland. Mast cells were found to be an essential component of the human pineal gland at any age. According to our data, the number of mast cells in the pineal gland increased with age. Mast cells were mostly located in the pineal stroma and did not tend to associate with concrements, cysts, or melanin accumulations. Mast cells in the pineal gland were predominantly non-degranulating, which indicated their inactive state. The detectability of mast cells in the pineal gland depended significantly on the used staining technique. The largest number of mast cells was revealed by tryptase immunohistochemical assay, which should be used to accurately determine the population of mast cells of the pineal gland.
Similar content being viewed by others
REFERENCES
Anisimov, V.N., Epiphysis and melatonin production, in Melatonin v norme i patologii (Melatonin in Normal and Pathological States), Komarov, F.I. et al., Eds., Moscow, 2004, pp. 7–19.
Anisimov, V.N., Age-related changes in the pineal gland, in Melatonin v norme i patologii (Melatonin in Normal and Pathological States), Komarov, F.I. et al., Eds., Moscow, 2004, pp. 20–33.
Anisimov, V.N., The effect of melatonin on aging, in Melatonin v norme i patologii (Melatonin in Normal and Pathological States), Komarov, F.I. et al., Eds., Moscow, 2004, pp. 223–236.
Anisimov, V.N., Epiphysis, biorhythms, and aging, Usp. Fiziol. Nauk, 2008, vol. 39, no. 4, pp. 40–65.
Atyashkin, D.A., Burtseva, A.S., and Sokolov, D.A., Evaluation of the effectiveness of mast cells detection in Mongolian gerbils jejunum mucosa using histochemical methods, Zh. Anat. Gistopatol., 2016, vol. 5, no. 4, pp. 85–89.
Bykov, V.L., Secretory mechanisms and secretory products of mast cells, Morfologiya, 1999, vol. 115, no. 2, pp. 72–79.
Zuev, V.A., Trifonov, N.I., Lin’kova, N.S., and Kvetnaya, T.V., Melatonin as a molecular marker of age pathology, Usp. Gerontol., 2017, vol. 30, no. 1, pp. 62–69.
Kvetnoi, I.M. and Ingel’, I.E., Hormonal function of nonendocrine cells: role of new biological phenomenon in the regulation of homeostasis, Bull. Exp. Biol. Med., 2000, vol. 130, no. 5, pp. 1027–1030.
Knyaz’kin, I.V., Role of extrapineal melatonin in the accelerated and premature aging in rats, Usp. Gerontol., 2008, vol. 21, no. 1, pp. 80–82.
Kutukova, N.A. and Nazarov, P.G., Role of mast cells in inflammation, tissue recovery, and fibrosis development, Tsitokiny Vospalenie, 2014, vol. 13, no. 2, pp. 11–20.
Kutukova, N.A., Nazarov, P.G., Kudryavtseva, G.V., and Shishkin, V.I., Mast cells and aging, Adv. Gerontol., 2017, vol. 7, no. 1, pp. 68–75.
Lillie, R.D., Histopathologic Technic and Practical Histochemistry, New York: McGraw-Hill, 1965, 3rd ed.
Turygin, V.V., Babik, T.M., and Boyakov, A.A., Characteristics of mast cells of the choroid plexus of the human brain ventricles during aging, Morfologiya, 2004, vol. 126, no. 6, pp. 61–62.
Fedorova, E.A., Grigorev, I.P., Syrtsova, M.A., et al., Identification of morphological features of mast cell degranulation in the choroid plexus of the human brain using various methods of staining and immunohistochemistry, Morfologiya, 2018, vol. 153, no. 2, pp. 70–75.
Yuneman, O.A., Morphological organization of the epiphysis and choroid plexus of the third ventricle of the human brain, Morfol. Ved., 2012, no. 3, pp. 97–100.
Anisimov, V.N. and Khavinson, V.Kh., Pineal peptides as modulators of aging, in Aging Interventions and Therapies, Rattan, S.I.S., Ed., Singapore: World Scientific, 2005, pp. 127–146.
Antić, S., Jovanović, I., Stefanović, N., et al., Morphology and histochemical characteristics of human pineal gland acervuli during the aging, Facta Univ., Ser.: Med. Biol., 2004, vol. 11, no. 2, pp. 63–68.
Atiakshin, D., Samoilova, V., Buchwalow, I., et al., Characterization of mast cell populations using different methods for their identification, Histochem. Cell Biol., 2017, vol. 147, no. 6, pp. 683–694.
Beil, W.J., Schulz, M., McEuen, A.R., et al., Number, fixation properties, dye–binding and protease expression of duodenal mast cells: comparisons between healthy subjects and patients with gastritis or Crohn’s disease, Histochem. J., 1997, vol. 29, no. 10, pp. 759–773.
Costantini, G., Interno ad alcune particolarità di struttura della glandola pineale, Pathologica, 1910, vol. 2, pp. 439–441.
Dropp, J.J., Mast cells in the human brain, Acta Anat., 1979, vol. 105, no. 4, pp. 505–513.
Gashev, A.A. and Chatterjee, V., Aged lymphatic contractility: recent answers and new questions, Lymphatic Res. Biol., 2013, vol. 11, no. 1, pp. 2–13.
Gomez, C.R., Nomellini, V., Faunce, D.E., and Kovacs, E.J., Innate immunity and aging, Exp. Gerontol., 2008, vol. 43, no. 8, pp. 718–728.
Hart, P.H., Grimbaldeston, M.A., Hosszu, E.K., et al., Age-related changes in dermal mast cell prevalence in BALB/c mice: functional importance and correlation with dermal mast cell expression of kit, Immunology, 1999, vol. 98, no. 3, pp. 352–356.
Kvetnoy, I.M., Extrapineal melatonin: location and role within diffuse neuroendocrine system, Histochem. J., 1999, vol. 31, no. 1, pp. 1–12.
Maldonado, M.D., Mora-Santos, M., Naji, L., et al., Evidence of melatonin synthesis and release by mast cells. Possible modulatory role on inflammation, Pharmacol. Res., 2010, vol. 62, no. 3, pp. 282–287.
Maślińska, D., Laure-Kamionowska, M., Deręgowski, K., and Maśliński, S., Association of mast cells with calcification in the human pineal gland, Folia Neuropathol., 2010, vol. 48, no. 4, pp. 276–282.
Mayrhofer, G., Fixation and staining of granules in mucosal mast cells and intraepithelial lymphocytes in the rat jejunum, with special reference to the relationship between the acid glycosaminoglycans in the two cell types, Histochem. J., 1980, vol. 12, no. 5, pp. 513–526.
Metcalfe, D.D., Baram, D., and Mekori, Y.A., Mast cells, Physiol. Rev., 1997, vol. 77, no. 4, pp. 1033–1079.
Nguyen, M., Pace, A.J., and Koller, B.H., Age-induced reprogramming of mast cell degranulation, J. Immunol., 2005, vol. 175, no. 9, pp. 5701–5707.
Pierpaoli, W., Dall’Ara, A., Pedrini, E., and Regelson, W., The pineal control of aging: the effects of melatonin and pineal grafting on the survival of older mice, Ann. N.Y. Acad. Sci., 1991, vol. 621, pp. 291–313.
Reiter, R.J., Tan, D.-X., and Kim, S.J., Augmentation of indices of oxidative damage in life-long melatonin-deficient rats, Mech. Ageing Dev., 1999, vol. 110, pp. 157–173.
Wernersson, S. and Pejler, G., Mast cell secretory granules: armed for battle, Nat. Rev. Immunol., 2014, vol. 14, no. 7, pp. 478–494.
Wingren, U. and Enerbäck, L., Mucosal mast cells of the rat intestine: a re-evaluation of fixation and staining properties, with special reference to protein blocking and solubility of the granular glycosaminoglycan, Histochem. J., 1983, vol. 15, no. 6, pp. 571–582.
Wu, Y.H. and Swaab, D.F., The human pineal gland and melatonin in aging and Alzheimer’s disease, J. Pineal Res., 2005, vol. 38, no. 3, pp. 145–152.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated by D. Timchenko
Rights and permissions
About this article
Cite this article
Fedorova, E.A., Sufieva, D.A., Grigorev, I.P. et al. Mast Cells of the Human Pineal Gland. Adv Gerontol 9, 62–66 (2019). https://doi.org/10.1134/S2079057019010053
Published:
Issue Date:
DOI: https://doi.org/10.1134/S2079057019010053