Advances in Gerontology

, Volume 2, Issue 3, pp 221–229 | Cite as

Effect of phenol inducing antioxidant responsive element on D. melanogaster lifespan

  • N. Ya. Weisman
  • E. B. Men’shchikova
  • N. K. Zenkov
  • N. V. Kandalintseva
  • M. D. Golubovsky


The effect of hydrophilic synthetic antioxidant TS-13 (sodium 3-(3′-tert-butyl-4′-hydroxyphenyl)propyl thiosulfonate) on the lifespan of different lines of Drosophila melanogaster under normal conditions and the survival in the oxidative stress induced by hydrogen peroxide and paraquat were studied. The addition of 1% TS-13 to diets extended the lifespan of males and females of the long-living Canton S line of D. melanogaster, had no effect on the lifespan of short-living Oregon R line and shortened the average lifespan of males of the lgl 558 OR/Cy line of D. melanogaster containing a heterozygous recessive lethal mutation of tumor suppressor. Under the conditions of oxidative stress induced by hydrogen peroxide, TS-13 increased the survival of Canton S males and Oregon R females; under the action of paraquat, the protective effect of antioxidant was manifested toward Canton S females and Oregon R flies of both sexes. Despite the fact that anti-aging and protective properties of synthetic phenolic antioxidant TS-13 strongly depend on the genotype and gender, under extreme conditions of oxidative stress. its positive effect is pronounced.


lifespan Drosophila melanogaster oxidative stress phenol antioxidan 


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  1. 1.
    Anisimov, V.N., Molekulyarnye i fiziologicheskie mekhanizmy stareniya (Molecular and Physiological Mechanisms of Senescence), St. Petersburg: Nauka, 2008, vol. 1.Google Scholar
  2. 2.
    Anisimov, V.N., Molekulyarnye i fiziologicheskie mekhanizmy stareniya (Molecular and Physiological Mechanisms of Senescence), St. Petersburg: Nauka, 2008, vol. 2.Google Scholar
  3. 3.
    Arutyunyan, A.V. and Kozina, L.S., Free Radical Oxidation Mechanisms and Its Role in Senescence, Uspekhi Gerontol., 2009, no. 1, pp. 104–116.Google Scholar
  4. 4.
    Weisman, N.Ya., Plyus, N., and Golubovsky, M.D., Ontogenesis and Haploadaptation of lgl Tumor Suppressor in D. melanogaster: An Increase of Survival and Life Duration at Stress, Ontogenez, 2007, vol. 38, no. 1, pp. 33–43.Google Scholar
  5. 5.
    Weisman, N.Ya. and Golubovsky, M.D., Genetic and Epigenetic Influence of lgl-Tumor Suppressor Mutation on Life Duration under Temperature Stress, Izv. RAN, Ser. Biol., 2009, no. 1, pp. 27–35.Google Scholar
  6. 6.
    Golubev, A.G., Biochemistry of Life Prolongation, Uspekhi Gerontol., 2003, no. 12, pp. 57–76.Google Scholar
  7. 7.
    Zenkov, N.K., Menshchikova, E.B., Kandalintseva, N.V., et al., Antioxidant and Antiinflammatory Activity of New Water-Soluble Sulfur-Containing Phenolic Compounds, Biokhimiya, 2007, vol. 72, no. 6, pp. 790–798.Google Scholar
  8. 8.
    Menshchikova, E.B., Lankin, V.Z., Zenkov, N.K., et al., Okislitel’nyi stress. Prooksidanty i antioksidanty (Oxidative Stress. Prooxidants and Antioxidants), Moscow: Slovo, 2006.Google Scholar
  9. 9.
    Oleynik, A.S., Kuprina, T.S., Pevneva, N.Yu., et al., Synthesis and Antiradical Activity of Sodium Hydroxyaryl Propyl Thiosulfonates and Sulfonates, Izv. AN, Ser. Khim., 2007, no. 6, pp. 1094–1101.Google Scholar
  10. 10.
    Tkachev, V.O., Menshchikova, E.B., and Zenkov, N.K., Mechanism of the Nrf2/Keap1/ARE Signaling System, Biokhimiya, 2011, vol. 76, no. 4, pp. 502–519.Google Scholar
  11. 11.
    Anisimov, V.N., Mylnikov, S.V., Oparina, T.I., and Khavinson, V.K., Effect of Melatonin and Pineal Peptide Preparation Epithalamin on Life Span and Free Radical Oxidation in Drosophila melanogaster, Mech. Aging Dev., 1997, vol. 97, pp. 81–91.PubMedCrossRefGoogle Scholar
  12. 12.
    Barone, M.C., Sykiotis, G.P., and Bohmann, D., Genetic Activation of Nrf2 Signaling is Sufficient to Ameliorate Neurodegenerative Phenotypes in a Drosophila Model of Parkinson’s Disease, Dis. Model. Mech., 2011, vol. 4.Google Scholar
  13. 13.
    Bayne, A.-C.V., Mockett, R.J., Orr, W.C., and Sohal, R.S., Enhanced Catabolism of Mitochondrial Superoxide/Hydrogen Peroxide and Aging in Transgenic Drosophila, Biochem. J., 2005, vol. 391, pp. 277–284.PubMedCrossRefGoogle Scholar
  14. 14.
    Brumby, A.M. and Richardson, H.E., Using D. melanogaster to Map Human Cancer Pathways, Nature Cancer Rev., 2005, vol. 5, pp. 626–639.CrossRefGoogle Scholar
  15. 15.
    Dinis-Oliveira, R.J., Duarte, J.A., Sanchez-Navar-ro, A., et al., Paraquat Poisonings: Mechanisms of Lung Toxicity, Clinical Features, and Treatment, Crit. Rev. Toxicol., 2008, vol. 38, pp. 13–71.PubMedCrossRefGoogle Scholar
  16. 16.
    Golubovsky, M.D., Weisman, N.Y., Arbeev, K.G., et al., Decrease in the lgl Tumor Suppressor Dose in Drosophila Increases Survival and Longevity in Stress Conditions, Exp. Geront., 2006, vol. 41, no. 9, pp. 819–827.CrossRefGoogle Scholar
  17. 17.
    Harman, D., Aging: A Theory Based on Free Radical and Radiation Chemistry, J. Geront., 1956, vol. 11, pp. 298–300.PubMedGoogle Scholar
  18. 18.
    Harman, D., Aging: Overview, Ann. N.Y. Acad. Sci., 2001, vol. 928, pp. 1–21.PubMedCrossRefGoogle Scholar
  19. 19.
    Honda, Y., Tanaka, M., and Honda, S., Redox Regulation, Gene Expression and Longevity, Geriat. Geront. Int., 2010, vol. 10,suppl. 1, pp. 59–69.CrossRefGoogle Scholar
  20. 20.
    Izmaylov, D.M. and Obukhova, L.K., Geroprotector Effectiveness of Melatonin: Investigation of Lifespan of Drosophila melanogaster, Mech. Aging Dev., 1999, vol. 106, pp. 233–240.PubMedCrossRefGoogle Scholar
  21. 21.
    Jimenez-Del-Rio, M., Guzman-Martinez, C., and Velez-Pardo, C., The Effects of Polyphenols on Survival and Locomotor Activity in Drosophila melanogaster Exposed to Iron and Paraquat, Neurochem. Res., 2010, vol. 35, pp. 227–238.PubMedCrossRefGoogle Scholar
  22. 22.
    Ma, Q., Battelli, L., and Hubbs, A.F., Multiorgan Autoimmune Inflammation, Enhanced Lymphoproliferation, and Impaired Homeostasis of Reactive Oxygen Species in Mice Lacking the Antioxidant-Activated Transcription Factor Nrf2, Am. J. Pathol., 2006, vol. 168, pp. 1960–1974.PubMedCrossRefGoogle Scholar
  23. 23.
    Maher, J. and Yamamoto, M., The Rise of Antioxidant Signaling—The Evolution and Hormetic Actions of Nrf2, Toxicol. Appl. Pharmacol., 2010, vol. 244, pp. 4–15.PubMedCrossRefGoogle Scholar
  24. 24.
    Perez, V.I., Bokov, A., van Remmen, H., et al., Is the Oxidative Stress Theory of Aging Dead? Biochim. Biophys. Acta, 2009, vol. 1790, pp. 1005–1014.PubMedCrossRefGoogle Scholar
  25. 25.
    Ristow, M. and Schmeisser, S., Extending Life Span by Increasing Oxidative Stress, Free Radic. Biol. Med., 2011, vol. 51, pp. 327–336.PubMedCrossRefGoogle Scholar
  26. 26.
    Sykiotis, G.P. and Bohmann, D., Keap1/Nrf2 Signaling Regulates Oxidative Stress Tolerance and Lifespan in Drosophila, Dev. Cell, 2008, vol. 14, pp. 76–85.PubMedCrossRefGoogle Scholar
  27. 27.
    Trinh, K., Andrews, L., Krause, J., et al., Decaffeinated Coffee and Nicotine-Free Tobacco Provide Neuroprotection in Drosophila Models of Parkinson’s Disease Through an NRF2-Dependent Mechanism, J. Neurosci., 2010, vol. 30, pp. 5525–5532.PubMedCrossRefGoogle Scholar
  28. 28.
    Underwood, B.R., Imarisio, S., Fleming, A., et al., Antioxidants Can Inhibit Basal Autophagy and Enhance Neurodegeneration in Models of Polyglutamine Disease, Hum. Mol. Genet., 2010, vol. 19, pp. 3413–3429.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • N. Ya. Weisman
    • 1
  • E. B. Men’shchikova
    • 2
  • N. K. Zenkov
    • 2
  • N. V. Kandalintseva
    • 3
  • M. D. Golubovsky
    • 4
  1. 1.Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia
  2. 2.Scientific Center of Clinical and Experimental Medicine, Siberian BranchRussian Academy of Medical SciencesNovosibirskRussia
  3. 3.Novosibirsk State Pedagogical UniversityNovosibirskRussia
  4. 4.Department of Molecular and Cell BiologyUniversity of CaliforniaBerkeleyUSA

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