Russian Journal of Biological Invasions

, Volume 3, Issue 2, pp 92–100 | Cite as

Study of microorganisms coming into the port of Vladivostok with ballast water of ships

  • L. S. Buzoleva
  • A. V. Letyagina
  • A. U. Zvyagincev
  • I. A. Kashin


The number and variety of heterotrophic and sanitary indicative bacteria in the ballast water of the Minotaur and Sunrise Wisteria tankers arriving from the ports of China and Japan are studied. It is found that ballast water coming into the port of Vladivostok from Japanese ports is less polluted than that from Chinese ports, which is defined as dirty and very dirty in the summer period. According to the pathogenicity of microflora, the donor ports can be arranged in the following series: Nantong > Longkou > Yangzhou > Laizhou > Mizushima > Iwakuni > Kawasaki. In ballast water of the Minotaur (from the Chinese ports), enterobacteria, the number of which exceeded the sanitary norms (by 40 or more times), and gram-negative mobile rod-shaped bacteria with an enzymatic type of metabolism resistant to heavy metals and antibiotics are singled out. For this reason, the habitats of these bacteria are characterized as insufficiently sated with oxygen and polluted by industrial and household water wastes. The sanitary indicator microorganisms are represented mainly by the following enterobacteria: Hafnia (37%), Enterobacter liqueface (22%), Citrobacter (15%), and Shigella (7%). According to data from a cytolytic test, the highest pathogenicity is found in Shigella, Hafnia, Citrobacter, and Salmonella.


ballast water port of Vladivostok heterotrophic microorganisms enterobacteria pathogenic and opportunistic pathogenic microflora 


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  1. Buzoleva, L.S. and Terekhova, V.E., Survival of Listeria monocytogenes and Yersinia pseudotuberculosis Bacterial Strains in Marine and River Water, Biologiya Morya, 2002, vol. 28, no. 4, pp. 286–290.Google Scholar
  2. Buzoleva, L.S., Bezverbnaya, I.P., Zhurvel’, E.V., and Kalita, E.G., Microbiological Assessment of Pollution of Far Seas in North-Western Pacific Ocean, Okeanologiya, 2006, vol. 46, no. 1, pp. 55–62.Google Scholar
  3. Burkholder, J.M., Hallegraeff, G.M., Melia, G., and Cohen, A., Phytoplankton and Bacterial Assemblages in Ballast Water of U.S. Military Ships as a Function of Port of Origin, Voyage Time, and Ocean Exchange Practices, Harmful Algae, 2007, vol. 6, no. 4, pp. 486–518.CrossRefGoogle Scholar
  4. Carlton, J.T., Man’s Role in Changing the Face of Ocean: Biological Invasions and Implications for Conservation of Near — Shore Environments, Conserv. Biol., 1990, no. 3, pp. 265–273.Google Scholar
  5. Dobbs, F.C., Diallo, A.A., Doblin, M.A., Drake, L.A., et al., Pathogens in Ships’ Ballast Water and Sediment Residuals, in Proceedings of the Third International Conference on Marine Bioinvasions, March 16–19, 2003, La Jolla, California, 2003, p. 29.Google Scholar
  6. Draft Resolution of the Government of the Russian Federation, Published on September 8, 2011.
  7. Drake, L.A., Baier, R.E., Dobbs, F.C., Doblin, M.A., et al., Potential Invasion of Microorganisms and Pathogens Via “Interior Hull Fouling”: Biofilms Inside Ballast-Water Tanks, in Proceedings of the Third International Conference on Marine Bioinvasions, March 16–19, 2003, La Jolla, California, 2003, p. 35.Google Scholar
  8. Drake, L.A., Doblin, M.A., and Dobbs, F.C., Potential Microbial Bioinvasions via Ships’ Ballast Water, Sediment, and Biofilm, Marine Pollution Bulletin, 2007, vol. 55,issues 7–9, pp. 333–341.CrossRefPubMedGoogle Scholar
  9. Ekologiya mikroorganizmov (Ecology of Microorganisms), Netrusov, A.I., Moscow: Akademiya, 2004, 272 p.Google Scholar
  10. Galil, B. and Hulsmann, N., Protist Transport via Ballast Water—Biological Classification of Ballast Tanks by Food Web Interactions, Eur. J. Potistol., 1997, no. 33, pp. 244–253.Google Scholar
  11. Gidrokhimicheskie pokazateli sostoyaniya okruzhayushchei sredy (Hydrochemical Parameters of Environment), Guseva, T.V., Ed., Moscow: Forum INFRA-M, 2007, 192 p.Google Scholar
  12. Hallegraeff, G.M., A Revive of Harmful Algal Blooms and Their Apparent Global Increase, Phycologia, 1993, vol. 32, no. 2, pp. 77–99.CrossRefGoogle Scholar
  13. Ivanov, V., Bacteriological Monitoring of Ships’ Ballast Water in Singapore and Its Potential Importance for the Management of Coastal Ecosystems, WIT Transactions on Biomedicine and Health, 2006, vol. 10, pp. 59–63.CrossRefGoogle Scholar
  14. Knight, I.T., Wells, C.S., Wiggins, B., Russell, H., et al., Detection and Enumeration of Fecal Indicators and Pathogens in the Ballast Water of Transoceanic Cargo Vessels Entering the Great Lakes, in Proceedings of the General Meeting of the ASM, Chicago, Illinois, 1999, p. 546.Google Scholar
  15. Kratkii opredelitel’ bakterii Bergi (Shorter Bergey’s Manual of Determinative Bacteriology), Holt, J., Ed., Moscow: Mir, 1980, 444 p.Google Scholar
  16. Lambert, R.J.W. and Pearson, J., Susceptibility Testing: Accurate and Reproducible Minimum Inhibitory Concentration (MIC) and Non-Inhibitory Concentration (NIC), Values, J. Appl. Microbiol., 2000, vol. 88, pp. 784–790.CrossRefPubMedGoogle Scholar
  17. McCarthy, S.A. and Khambaty, F.M., International Dissemination of Epidemic Vibrio cholerae by Cargo Ship Ballast and Other Nonpotable Waters, Applied and Environmental Microbiology, 1994, vol. 60, issue 7, pp. 2597–2601.Google Scholar
  18. Metody obshchei bakteriologii (Manual of Methods for General Bacteriology), Gerhardt, P., Moscow: Mir, 1983, vol. 1, 536 p.Google Scholar
  19. Metody obshchei bakteriologii (Manual of Methods for General Bacteriology), Gerhardt, P., Moscow: Mir, 1984, vol. 3, 264 p.Google Scholar
  20. Mezhdunarodnaya Konventsiya o kontrole sudovykh ballastnykh vod i osadkov, i upravlenii imi 2004 goda (International Convention on Monitoring of Ships’ Ballast Water and Sediment Residuals, and Their Management in 2004), St. Petersburg: TsNIIMF, 2005, 120 p.Google Scholar
  21. Mills, E.L., Leach, J.H., Carlton, J.T., and Secor, C.L., Exotic Species in the Great Lakes: A History of Biotic Crises and Anthropogenic Introductions, J. Great Lakes Res., 1993, no. 19, pp. 1–57.Google Scholar
  22. Obshchaya i sanitarnaya mikrobiologiya s tekhnikoi mikrobiologicheskikh issledovanii (General and Sanitary Microbiology with Technique of Microbiological Studies), Labinskaya, A.S., Ed., Tatarstan: Meditsina, 2004, 575 p.Google Scholar
  23. Polyak, M.S., Opredelenie chuvstvitel’nosti mikroorganizmov k protivomikrobnym preparatam metodom diskov (Determination of Sensitivity of Microorganisms to Antimicrobial Preparations by Disk Diffusion Method), St. Petersburg: NITSF, 1997, 20 p.Google Scholar
  24. Pozdeev, O.K., Meditsinskaya mikrobiologiya (Medical Microbiology), Moscow: Geotar-Med, 2002, 768 p.Google Scholar
  25. Sanitary Rules and Standards: SanPiN Drainage of Habituated Areas, Sanitary Protection of Water Objects. Hygienic Standards to Protection of Surface Waters. Approved by Chief State Sanitary Physician of Russian Federation on July 22, 2000, Moscow: Minzdrav RF, 2000.Google Scholar
  26. Selifonova, Zh.P., Marine Bioinvasions in Water of the Novorossiysk Black Sea Port, Biologiya Morya, 2009, vol. 35, no. 3, pp. 212–219.Google Scholar
  27. Selifonova, Zh.P., Kontrol’ sudovykh ballastnykh vod kak metod predotvrashcheniya biologicheskogo zagryazneniya morskoi sredy: Metodicheskoe posobie (Manual on Monitoring of Ships’ Ballast Water as the Method for Prevention of Biological Pollution of Marine Environment), Apatity: Kol’sk. Nauch. Tsentr RAN, 2010, 87 p.Google Scholar
  28. Somov, G.P., and Buzoleva, L.S., Adaptatsiya patogennykh bakterii k abioticheskim faktoram okruzhayushchei sredy (Adaptation of Pathogenic Bacteria to Abiotic Environmental Factors), Vladivostok: Primpoligrafkombinat, 2004, 167 p.Google Scholar
  29. Thomson, F.K., Heinemann, S.A., and Dobbs, F.C., Patterns of Antibiotic Resistance in Cholera Bacteria Isolated From Ships’ Ballast Water, in Proceedings of the Third International Conference on Marine Bioinvasions, March 16–19, 2003, La Jolla, California, 2003, p. 118.Google Scholar
  30. Tsyban’, A.V., Ivanitsa, V.A., and Khudchenko, G.I., Biological Characteristics of Marine Microorganisms, Issledovanie ekosistem Beringova i Chukotskogo morei (Investigation of Ecosystems of the Bering and Black Seas), St. Petersburg: Gidrometeoizdat, 1992, issue 3, pp. 193–212.Google Scholar
  31. Vashchenko, M.A., Pollution of the Peter the Great Gulf in the Sea of Japan and Its Biological Consequences, Biologiya Morya, 2000, vol. 26, no. 3, pp. 149–159.Google Scholar
  32. Williams, R.J., Griffiths, F.B., Van der Wal, E.J., and Kelly, J., Cargo Vessel Ballast Water as a Vector for the Transport of Non-Indigenous Marine Species, Est. Coast. Shelf Sci., 1988, no. 26, pp. 409–420.Google Scholar
  33. Wommack, K.E. and Colwell, R.R., Virioplankton: Viruses in Aquatic Ecosystems, Microbiol. Mol. Biol. Rev., 2000, vol. 64, no. 1, pp. 69–114.CrossRefPubMedGoogle Scholar
  34. Youchimizu, M. and Kimura, T., Study of Intestinal Microflora of Salmonids, Fish. Pathol., 1976, vol. 10, no. 2, p. 243.Google Scholar
  35. Zvyagincev, A.U., Ivin, V.V., Kashin, I.A., Orlova, T.Yu., Selina, M.S., Kas’yan, V.V., et al., Population of Ballast Water in Vladivostok Port, Biologiya Morya, 2009a, vol. 35, no. 1, pp. 29–40.Google Scholar
  36. Zvyagincev, A.U., Ivin, V.V., and Kashin, I.A., Metodicheskie rekomendatsii po issledovaniyu sudovykh ballastnykh vod pri monitoringe morskikh bioinvazii (Methodological Recommendations for Study of Ships’ Ballast Water and Monitoring Marine Bioinvasions), Vladivostok: Dal’nauka, 2009b, 120 p.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • L. S. Buzoleva
    • 1
  • A. V. Letyagina
    • 2
  • A. U. Zvyagincev
    • 3
  • I. A. Kashin
    • 3
  1. 1.Research Institute of Epidemiology and Microbiology, Siberian BranchRussian Academy of Medical SciencesVladivostokRussia
  2. 2.Far Eastern Federal UniversityVladivostokRussia
  3. 3.Institute of Marine Biology, Far Eastern BranchRussian Academy of SciencesVladivostokRussia

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