Inland Water Biology

, Volume 8, Issue 3, pp 236–241 | Cite as

Viruses in bottom sediments in a eutrophic reservoir (Ivankovo Reservoir, Upper Volga)

  • A. I. Kopylov
  • E. A. Zabotkina
  • A. V. Romanenko
Aquatic Microbiology

Abstract

The total number of viruses, morphological and size composition of viriobenthos, number of bacterial cells infected by viruses, number of mature phages inside bacterial cells, and virus-induced mortality of bacteriobenthos have been estimated in bottom sediments of the Ivankovo Reservoir. The total number of viriobenthos in the reservoir range from 10.3 to 83.2 (31.5 ± 6.2 on average) billion particles/cm3. The total number of virus-to-bacteria ratio in the Ivan’kovo reservoir varies between 0.3 and 1.7 (0.7 ± 0.1 on average). A positive correlation between viral and bacterial abundance is found (R = 0.61, p = 0.05). In different parts of the reservoir, viral infection of bacteria is not observed or is very low. The number of infected bacteria ranges from 1.0 to 3.5% (1.7 ± 0.2% on average) of the total viriobenthos abundance and mortality due to viral lysis accounts for 1.0–3.7% (1.8 ± 0.2% on average) of the total bacterial mortality. The number of phages found inside bacterial cells average 8 ± 1 phages/cell. Thus, a phenomenon known as “infection paradox” is observed in the Ivankovo Reservoir when, at a very high viral abundance, no viral infection of bacteria is recorded or it was very low.

Keywords

viriobenthos bacteria infected by viruses virus-induced mortality of bacteriobenthos reservoir 

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References

  1. 1.
    Kopylov, A.I., Kosolapov, D.B., and Zabotkina, E.A., Distribution of viruses and their impact on bacterioplankton in mesotrophic and eutrophic reservoirs, Inland Water Biol., 2008, Vol. 1, No. 1, pp. 46–53.CrossRefGoogle Scholar
  2. 2.
    Kosolapov, D.B., Krylova, I.N., and Kopylov, A.I., Distribution and activity of bacteriobenthos in the Upper Volga Reservoirs, Water Resour., 2005, Vol. 32, No. 4, pp. 445–455.CrossRefGoogle Scholar
  3. 3.
    Bettarel, Y., Bouvy, M., Dumont, C., and Sime-Ngando, T., Virus–bacterium interactions in water and sediment of West African inland aquatic systems, Appl. Environ. Microbiol., 2006, Vol. 72, pp. 5274–5282.PubMedCentralCrossRefPubMedGoogle Scholar
  4. 4.
    Binder, B., Reconsidering the relationship between virally induced bacterial mortality and frequency of infected cells, Aquat. Microbial. Ecol., 1999, Vol. 18, pp. 207–215.CrossRefGoogle Scholar
  5. 5.
    Danovaro, R., Corinakdsi, C., Filippini, M., et al., Viriobenthos in freshwater and marine sediments: a review, Freshwater Ecol., 2008, Vol. 53, pp. 1186–1213.CrossRefGoogle Scholar
  6. 6.
    Danovaro, R., Manini, E., and Dell’Anno, A., Higher abundance of bacteria than viruses in deep Mediterranean sediments, Appl. Environ. Microbiol., 2002, Vol. 68, pp. 1468–1472.PubMedCentralCrossRefPubMedGoogle Scholar
  7. 7.
    Filippini, M., Boesing, N., Bettarel, Y., et al., Infection paradox: high abundance but low impact of freshwater benthic viruses, Appl. Environ. Microbiol., 2006, Vol. 72, pp. 4893–4898.PubMedCentralCrossRefPubMedGoogle Scholar
  8. 8.
    Fischer, U.R., Wieltschnig, C., Kirschner, A.K.T., and Velimirov, B., Does virus-induced lysis contribute to bacterial mortality in the oxygenated sediment layer of shallow oxbow lake?, Appl. Environ. Microbiol., 2003, Vol. 69, pp. 5281–5289.PubMedCentralCrossRefPubMedGoogle Scholar
  9. 9.
    Glud, R.N. and Middelboe, M., Virus and bacteria dynamics of a coastal sediment: implication for benthic carbon cycling, Limnol., Oceanogr, 2004, Vol. 49, pp. 2073–2081.CrossRefGoogle Scholar
  10. 10.
    Hewson, I. and Fuhrman, J.A., Viriobenthos production and virioplankton sorptive scavenging by suspended sediment particles in coastal and pelagic waters, Microb. Ecol., 2003, Vol. 46, pp. 337–347.CrossRefPubMedGoogle Scholar
  11. 11.
    Hewson, L., O’Nell, J.M., Fuhrman, J.A., and Dennison, W.C., Virus-like particle distribution and abundance in sediments and overlying waters along eutrophication gradients in two subtropical estuaries, Limnol., Oceanogr, 2001, Vol. 46, pp. 1734–1746.CrossRefGoogle Scholar
  12. 12.
    Maranger, R. and Bird, D.F., High concentrations of viruses in the sediments of Lac Gilbert, Quebec, Microb. Ecol., 1996, Vol. 31, pp. 141–151.Google Scholar
  13. 13.
    Mei, M.L. and Danovaro, R., Virus production and life strategies in aquatic sediments, Limnol., Oceanogr., 2004, Vol. 49, pp. 459–470.CrossRefGoogle Scholar
  14. 14.
    Middelboe, M., Glud, R.N., and Finster, K., Distribution off viruses and bacteria in relation to diagenetic activity in an estuarine sediment, Limnol., Oceanogr., 2003, Vol. 48, pp. 1447–1456.CrossRefGoogle Scholar
  15. 15.
    Noble, R.T. and Fuhrman, J.A., Use of SYBR Green for rapid epifluorescence count of marine viruses and bacteria, Aquat. Microb. Ecol., 1998, Vol. 14, pp. 113–118.CrossRefGoogle Scholar
  16. 16.
    Paul, J.H., Rose, J.B., Jiano, S.C., et al., Distribution of viral abundance in the reef environment of Key Largo, Florida, Appl. Environ. Microbiol., 1993, Vol. 59, pp. 718–724.PubMedCentralPubMedGoogle Scholar
  17. 17.
    Porter, K.G. and Feig, Y.S., The use of dapi for identifying and counting of aquatic microflora, Limnol., Oceanogr., 1980, Vol. 25, No. 5, pp. 943–948.CrossRefGoogle Scholar
  18. 18.
    Ricciardi-Rigauli, M., Bird, D.F., and Prairie, Y.T., Changes in sediment viral and bacterial abundances with hypolimnetic oxygen depletion in a shallow eutrophic Lac Brome (Quebec, Canada), Can. J. Fish. Aquat. Sci., 2000, Vol. 56, pp. 1284–1290.CrossRefGoogle Scholar
  19. 19.
    Simek, K., Pernthaler, I., Weinbauer, M.G., et al., Changes in bacterial community composition dynamics and viral mortality rates associated with enhanced flagellate grazing in a mesoeutrophic reservoir, Appl. Environ. Microbiol., 2001, Vol. 67, No. 6, pp. 2723–2733.PubMedCentralCrossRefPubMedGoogle Scholar
  20. 20.
    Slovâcková, H., Study of the ecological role of viruses and bacteria in aquatic ecosystems, Dis. Thes., Brno, 2008.Google Scholar
  21. 21.
    Weinbauer, M.G., Ecology of prokaryotic viruses, FEMS Microbiol. Rev., 2004, Vol. 28, No. 2, pp. 127–181.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • A. I. Kopylov
    • 1
  • E. A. Zabotkina
    • 1
  • A. V. Romanenko
    • 1
  1. 1.Papanin Institute for Biology of Inland WatersRussian Academy of SciencesBorok, Nekouzskii raion, Yaroslavl oblastRussia

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