Inland Water Biology

, Volume 3, Issue 1, pp 90–95 | Cite as

Influence of biogenic metals (Cu, Zn) on the activity of carbohydrases in juvenile fish in vitro

Aquatic Toxicology

Abstract

It was revealed that intestine and whole-body amylolytic activity (AA) in juvenile fish serving as potential feeding items for piscivores significantly decreases in the presence of Cu and Zn ions within a wide range (0.1–25 mg/l) of concentrations in vitro. In most of the studied fish species, Cu and Zn decrease the activities of carbohydrases in the intestine mucosa stronger than in the whole-body. On the other hand, in piscivore-facultative benthivore perch, which have the least AA, the inhibiting effect of Cu and Zn ions is 1.5 to 2 times higher in the whole body than in the intestine. The study data suggest that Cu and Zn ions at concentrations found in organisms that serve as fish food may not only reduce the rate of initial stages of carbohydrates hydrolysis in the juvenile fish intestine, but also considerably decrease the potential contribution of the food objects to the digestion processes in typical and facultative piscivores.

Key words

fish digestion carbohydrases heavy metals copper zinc 

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References

  1. 1.
    Alabaster, D. and Lloid, R., Kriterii kachestva vody dlya presnovodnykh ryb (Criteria of Water Quality for Fresh Water Fish), London, 1980, Moscow: Legk. Pishch. Prom., 1984.Google Scholar
  2. 2.
    Volkova, I.V., Activity of Digestive Enzymes of Phytophagous Fishes at Different Stages of Ontogeny, Extended Abstract of Cand. Sci. (Biol.) Dissertation: Astrakhan’, 1999.Google Scholar
  3. 3.
    Golovanova, I.L., Influence of Natural and Anthropogenic Factors on the Activity of Carbohydrases of Young Fish, Biologiya Vnutr. Vod., 2000, no. 1, pp. 143–148.Google Scholar
  4. 4.
    Golovanova, I.L., Influence of Natural and Anthropogenic Factors on Hydrolysis of Carbohydrates in Freshwater Teleost Fishes and in Their Feeding Subjects, Extended Abstract of Doctoral (Biol). Dissertation, St. Petersburg, 2006.Google Scholar
  5. 5.
    Kuz’mina, V.V., Contribution of Induced Autolylsis to Digestive Processes of Secondary Consumers as Exemplified by Aquatic Organisms, Dokl. Akad. Nauk, 2000, vol. 339, no. 1, pp. 172–174.Google Scholar
  6. 6.
    Kuz’mina, V.V. and Ushakova, N.V., Effect of Temperature, pH, and Heavy Metals (Copper and Zinc) on the Activity of Proteinases of Potential Feeding Objects of Typical and Facultative Ichthyophages, Vopr. Ikhtiol., 2007, vol. 47, no. 6, pp. 837–846.Google Scholar
  7. 7.
    Kuz’mina, V.V., Shishin, M.M., Koryukaeva, N.V., et al., Effect of Copper and Zinc on the Efficiency of Hydrolysis of Protein Components of Food in Some Species of Freshwater Teleost Fishes in Vitro, Biol. Vnutr. Vod, 2005, no. 4, pp. 84–92.Google Scholar
  8. 8.
    Malysheva, T.D. and Vasilevskii, V.S., Influence of Excessive Intestine Zinc upon Activity of Common Carp Digestive Enzymes, Gidrobiol. Zh., 1992, vol. 28, no. 4, pp. 45–52.Google Scholar
  9. 9.
    Nevalennyi, A.N., Tuktarov, A.V., and Bednyakov, D.A., Funktsional’naya Organizatsiya i Adaptivnaya Regulyatsiya Protsessov Pishchevareniya u Ryb. (Functional Organization and Adaptive Regulation of Digestive Processes in Fish) Astrakhan: Astrakhan. Gos. Tekhn. Univ., 2003.Google Scholar
  10. 10.
    Ostroumova, I.N., Biologicheskie Osnovy Kormleniya Ryb. (Biological Bases of Fish Feeding), St.-Petersburg: Gos. NII Ozer. Rech. Ryb. Khoz., 2001.Google Scholar
  11. 11.
    Perevoznikov, M.A. and Bogdanova, E.A., Tyazhelye Metally v Presnovodnykh Ekosistemakh (Heavy Metals in Freshwater Ecosystems), St.-Petersburg: Gos. NII Ozer. Rech. Ryb. Khoz., 1999.Google Scholar
  12. 12.
    Sobolev, K.D., Pollution with Heavy Metals of Natural and Artificial Forage and Its Effect on Fishes under Conditions of Discharge Warm Waters, Extended Abstract of Cand. Sci. (Biol.) Dissertation: St.-Petersburg, 2006.Google Scholar
  13. 13.
    Stolyar, O.B., Kurant, V.Z., Khomenchuk, V.A., and Grubinko, V.V., Characteristic of Low-Molecular-Weight Sulfur-Containing Compounds of Carp Hepatopancreas in Copper and Zinc Intoxication, Gidrobiol. Zh., 2003, vol. 39, no. 4, pp. 91–98.Google Scholar
  14. 14.
    Tuktarov, A.V., Effect of Heavy Metals on the Digestive-Transport Function of the Intestine of Acipenseridae, Extended Abstract of Cand. Sci. (Biol.) Dissertation: Astrakhan’, 2002.Google Scholar
  15. 15.
    Ugolev, A.M. and Iezuitova, N.N., Determination of Activity of Invertase and Other Disaccharidases, in Issledovanie pishchevaritel’nogo apparata u cheloveka (Study of the Digestive Apparatus in Humans), Leningrad: Nauka, 1969, pp. 192–196.Google Scholar
  16. 16.
    Ugolev, A.M. and Kuz’mina, V.V., Pishchevaritel’nye Protsessy i Adaptatsii u Ryb (Digestive Processes and Adaptations in Fish), St.-Petersburg: Gidrometeoizdat, 1993.Google Scholar
  17. 17.
    Ugolev, A.M. and Tsvetkova, V.A., Induced Autolysis as an Important Mechanism of Initial Digestive Stages under Natural Conditions, Fiziol. Zh., 1984, vol. 70, no. 11, pp. 1542–1550.Google Scholar
  18. 18.
    Bury, N.R., Walker, P.A., and Glover, C.N., Nutritive Metal Uptake in Teleost Fish, J. Exp. Biol., 2003, vol. 206, no. 1, pp. 11–23.CrossRefPubMedGoogle Scholar
  19. 19.
    De Boeck, G., Ngo, T.T.H., Van Campenhout, K., and Blust, R., Differential Metallothionein Inductions Patterns in Three Freshwater Fish during Sublethal Copper Exposure, Aquat. Toxicol., 2003, vol. 65, no. 4, pp. 413–424.PubMedGoogle Scholar
  20. 20.
    Sokal, R.R. and Rohlf, F.J., Biometry, New York: Freeman, 1995.Google Scholar
  21. 21.
    Sun, L.-T. and Jeng, S.-S., Accumulation of Zinc from Diet and Its Release in Common Carp, Fish Physiol. Biochem., 1999, vol. 20, no. 4, pp. 313–324.CrossRefGoogle Scholar
  22. 22.
    Watanabe, T., Kiron, V., and Satoh, S., Trace Minerals in Fish Nutrition, Aquaculture, 1997, vol. 151, no. 14, pp. 185–207.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Papanin Institute for the Biology of Inland WatersRussian Academy of SciencesBorok, Yaroslavskaya oblast, Nekouzskii raionRussia

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