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Nanotechnologies in Russia

, Volume 10, Issue 1–2, pp 140–148 | Cite as

Investigation into size distribution of carbon nanoparticles covalently functionalized with proteins

  • M. B. Raev
  • P. V. Khramtsov
  • M. S. Bochkova
Article

Abstract

The sizes of carbon nanoparticles covalently functionalized with streptococcal protein G and streptavidin are investigated. Such nanoparticles are used to develop point-of-care tests. Atomic force microscopy (AFM) and dynamic light scattering (DLS) are used to measure the particle sizes. The average size of the carbon nanoparticles conjugate with protein G was 98 nm (min = 56 nm, max = 142 nm) according to the AFM data and 139 nm according to DLS. The sizes of four carbon-streptavidin conjugates with a storage period from 1 to 22 years are compared by DLS. DLS results have revealed that the partial aggregation of the particles (increasing the average size by 10–20%) probably occurs during the first 3 years of conjugate storage. The further storage of the functionalized nanoparticles does not lead to any alteration in the particle size. Additionally, the regularities of the changes in the particle sizes at the different functionalization steps are studied.

Keywords

Atomic Force Microscopy Particle Size Dynamic Light Scattering Amorphous Carbon Carbon Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    S. Rashdan, et al., “1 — nanoparticles for biomedical applications: current status, trends and future challenges,” in Biomaterials and Medical Tribology, Ed. by J. P. Davim (Woodhead Publ., 2013), pp. 1–132.CrossRefGoogle Scholar
  2. 2.
    C. Kleinstreuer, E. Childress, and A. Kennedy, “Targeted drug delivery multifunctional nanoparticles and direct micro-drug delivery to tumors,” in Transport in Biological Media, Ed. by S. M. Becker and A. Kuznetsov (Elsevier, 2013), Chapter 10, pp. 391–416.CrossRefGoogle Scholar
  3. 3.
    S. Thatai, et al., “Nanoparticles and core-shell nanocomposite based new generation water remediation materials and analytical techniques: a review,” Microchem. J. 116, 62–76 (2014).CrossRefGoogle Scholar
  4. 4.
    C. Cha, et al., “Carbon-based nanomaterials: multifunctional materials for biomedical engineering,” ACS Nano 7(4), 2891–2897 (2013).CrossRefGoogle Scholar
  5. 5.
    O. Gohardani, M. C. Elola, and C. Elizetxea, “Potential and prospective implementation of carbon nanotubes on next generation aircraft and space vehicles: a review of current and expected applications in aerospace sciences,” Progr. Aerospace Sci. 70, 42–68 (2014).CrossRefGoogle Scholar
  6. 6.
    S. Kruss, et al., “Carbon nanotubes as optical biomedical sensors,” Adv. Drug Delivery Rev. 65(15), 1933–1950 (2013).CrossRefGoogle Scholar
  7. 7.
    G. A. Posthuma-Trumpie, et al., “Amorphous carbon nanoparticles: a versatile label for rapid diagnostic (immuno)assays,” Anal. Bioanal. Chem. 402(2), 593–600 (2012).CrossRefGoogle Scholar
  8. 8.
    R. Wilson, “The use of gold nanoparticles in diagnostics and detection,” Chem. Soc. Rev. 37(9), 2028–2045 (2008).CrossRefGoogle Scholar
  9. 9.
    J. A. Molina-Bolívar, and F. Galisteo-González, “Latex immunoagglutination assays,” J. Macromolec. Sci. C 45(1), 59–98 (2005).CrossRefGoogle Scholar
  10. 10.
    E. F. Ullman, “Homogeneous immunoassays,” in The Immunoassay Handbook, 4th ed., Ed. by D. Wild (Elsevier, 2013), Chapter 2.3, pp. 67–87.CrossRefGoogle Scholar
  11. 11.
    X. He, et al., “Water soluble carbon nanoparticles, hydrothermal synthesis and excellent photoluminescence properties,” Colloids Surf. 87(2), 326–332 (2011).CrossRefGoogle Scholar
  12. 12.
    W. Chen, “Nanosized carbon particles from natural gas soot,” Chem. Mater. 21(13), 2803–2809 (2009).CrossRefGoogle Scholar
  13. 13.
    A. van Amerongen, et al., “Colloidal carbon particles as a new label for rapid immunochemical test methods: quantitative computer image analysis of results,” J. Biotechnol. 30(2), 185–195 (1993).CrossRefGoogle Scholar
  14. 14.
    M. Raev, “Test-System for determining antibodies to HIV-1,2,” in Proc. Conf. “Topical Problems of Theoretical and Practical Immunology” (Perm, 1994), pp. 48–49.Google Scholar
  15. 15.
    M. Raev, et al., “Non-ferment test system for toolless detection of antibodies to Yersinia Pseudotuberculosis species-specific proteins based on nanosized carbon particles,” Dokl. Akad. Nauk 451(6), 695–698 (2013).Google Scholar
  16. 16.
    M. Raev, M. Bochkova, and P. Khramtsov, “Dot-analytical system for detecting antibodies to Treponema Pallidum,” Dokl. Akad. Nauk 457(4), 491–493 (2014).Google Scholar
  17. 17.
    P. Khramtsov, M. Bochkova, and M. Raev, “System for estimationing individualy an immunity stress to pertussis,” in Proc. Jubilee Sci.-Pract. Conf. “40th Anniversary of Scientific Research Institute for Specially Pure Biological Products” (St. Petersburg, 2014), Vol. 13, No. 1, p. 128.Google Scholar
  18. 18.
    M. Raev, et al., “The way to create and apply a universal test system by using nonenzymatic diagnosticums for toolless estimation of specific antibodies level,” Biotekhnologiya, No. 1, 84–88 (2006).Google Scholar
  19. 19.
    M. Raev, in Nanotechnologies in Toolless Immune Analytics, Ed. by V. A. Demakov (Printing and Publication Department of Ural Branch RAS, Yekaterinburg, 2012) [in Russian].Google Scholar
  20. 20.
    V. Timganova, M. Bochkova, and M. Raev, “Stability of structural-functional properties of carbon diagnosticums,” Dokl. Akad. Nauk 450(4), 492–495 (2013).Google Scholar
  21. 21.
    P. Munro, P. Dunnil, and M. Lilly, “Casein hydrolysis in stirred tank reactors using chymotrypsin immobilized on magnetic supports,” Biotechnol. Bioeng. 23(4), 677–689 (1981).CrossRefGoogle Scholar
  22. 22.
    M. Aizawa, R. Coughtin, and M. Charles, “Activation of alumina with bovine serum albumin for immobilizing enzymes,” Biotechnol. Bioeng. 17(9), 1369–1372 (1975).CrossRefGoogle Scholar
  23. 23.
    A. Yan, et al., “Biocompatible, hydrophilic, supramolecular carbon nanoparticles for cell delivery,” Adv. Mater. 18(18), 2373–2378 (2006).CrossRefGoogle Scholar
  24. 24.
    Y. Li, et al., “Carbon nanoparticles from corn stalk soot and its novel application as stationary phase of hydrophilic interaction chromatography and per aqueous liquid chromatography,” Anal. Chim. Acta 726, 102–108 (2012).CrossRefGoogle Scholar
  25. 25.
    H. Zhao, J. Tian, and X. Quan, “A graphene and multienzyme functionalized carbon nanosphere-based electrochemical immunosensor for microcystin-LR detection,” Colloids Surf. B: Biointerfaces 103, 38–44 (2013).CrossRefGoogle Scholar
  26. 26.
    W.-K. Oh, H. Yoon, and J. Jang, “Characterization of surface modified carbon nanoparticles by low temperature plasma treatment,” Diamond Related Mater. 18(10), 1316–1320 (2009).CrossRefGoogle Scholar
  27. 27.
    E. Linares, et al., “Enhancement of the detection limit for lateral flow immunoassays: evaluation and comparison of bioconjugates,” J. Immunol. Methods 375(1–2), 264–270 (2012).CrossRefGoogle Scholar
  28. 28.
    T. Muthukumar, et al., “Bio-modified carbon nanoparticles loaded with methotrexate possible carrier for anticancer drug delivery,” Mater. Sci. Eng. C 36, 14–19 (2014).CrossRefGoogle Scholar
  29. 29.
    RF Patent No. 20899212 (1997).Google Scholar
  30. 30.
    H. Li, et al., “Nucleic acid detection using carbon nanoparticles as a fluorescent sensing platform,” Chem. Commun. 47(3), 961–963 (2011).CrossRefGoogle Scholar
  31. 31.
    A. Bootz, et al., “Comparison of scanning electron microscopy, dynamic light scattering and analytical ultracentrifugation for the sizing of poly(butyl cyanoacrylate) nanoparticles,” Europ. J. Pharm. Biopharm. 57(2), 369–375 (2004).CrossRefGoogle Scholar
  32. 32.
    V. Vogel, et al., “Metallo-supramolecular micelles: studies by analytical ultracentrifugation and electron microscopy,” J. Polym. Sci. A Polym. Chem. 41(20), 3159–3168 (2003).CrossRefGoogle Scholar
  33. 33.
    ZetaSizer Nano Series User Manual, Chapter 5. http://www.biophysics.bioc.cam.ac.uk/files/Zetasizer_Nano_user_manual_Man0317-1.1.pdf (Assessed 25.09.2014).

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • M. B. Raev
    • 1
  • P. V. Khramtsov
    • 1
  • M. S. Bochkova
    • 1
  1. 1.Institute of Ecology and Genetics of Microorganisms, Ural BranchRussian Academy of SciencesPermRussia

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