Investigation into size distribution of carbon nanoparticles covalently functionalized with proteins
- 45 Downloads
The sizes of carbon nanoparticles covalently functionalized with streptococcal protein G and streptavidin are investigated. Such nanoparticles are used to develop point-of-care tests. Atomic force microscopy (AFM) and dynamic light scattering (DLS) are used to measure the particle sizes. The average size of the carbon nanoparticles conjugate with protein G was 98 nm (min = 56 nm, max = 142 nm) according to the AFM data and 139 nm according to DLS. The sizes of four carbon-streptavidin conjugates with a storage period from 1 to 22 years are compared by DLS. DLS results have revealed that the partial aggregation of the particles (increasing the average size by 10–20%) probably occurs during the first 3 years of conjugate storage. The further storage of the functionalized nanoparticles does not lead to any alteration in the particle size. Additionally, the regularities of the changes in the particle sizes at the different functionalization steps are studied.
KeywordsAtomic Force Microscopy Particle Size Dynamic Light Scattering Amorphous Carbon Carbon Particle
Unable to display preview. Download preview PDF.
- 14.M. Raev, “Test-System for determining antibodies to HIV-1,2,” in Proc. Conf. “Topical Problems of Theoretical and Practical Immunology” (Perm, 1994), pp. 48–49.Google Scholar
- 15.M. Raev, et al., “Non-ferment test system for toolless detection of antibodies to Yersinia Pseudotuberculosis species-specific proteins based on nanosized carbon particles,” Dokl. Akad. Nauk 451(6), 695–698 (2013).Google Scholar
- 16.M. Raev, M. Bochkova, and P. Khramtsov, “Dot-analytical system for detecting antibodies to Treponema Pallidum,” Dokl. Akad. Nauk 457(4), 491–493 (2014).Google Scholar
- 17.P. Khramtsov, M. Bochkova, and M. Raev, “System for estimationing individualy an immunity stress to pertussis,” in Proc. Jubilee Sci.-Pract. Conf. “40th Anniversary of Scientific Research Institute for Specially Pure Biological Products” (St. Petersburg, 2014), Vol. 13, No. 1, p. 128.Google Scholar
- 18.M. Raev, et al., “The way to create and apply a universal test system by using nonenzymatic diagnosticums for toolless estimation of specific antibodies level,” Biotekhnologiya, No. 1, 84–88 (2006).Google Scholar
- 19.M. Raev, in Nanotechnologies in Toolless Immune Analytics, Ed. by V. A. Demakov (Printing and Publication Department of Ural Branch RAS, Yekaterinburg, 2012) [in Russian].Google Scholar
- 20.V. Timganova, M. Bochkova, and M. Raev, “Stability of structural-functional properties of carbon diagnosticums,” Dokl. Akad. Nauk 450(4), 492–495 (2013).Google Scholar
- 29.RF Patent No. 20899212 (1997).Google Scholar
- 33.ZetaSizer Nano Series User Manual, Chapter 5. http://www.biophysics.bioc.cam.ac.uk/files/Zetasizer_Nano_user_manual_Man0317-1.1.pdf (Assessed 25.09.2014).