An investigation into the interaction between carbon-based nanomaterials and Escherichia coli cells using atomic force microscopy
Using atomic force microscopy (AFM), the nature of the contact between a wide range of carbonbased nanomaterials (CBNs) and Escherichia coli cells is described and the consequences of such an interaction are estimated. It is shown that the contact of multiwall and a number of single-wall carbon nanotubes with the surface of a model microorganism carries a probabilistic nature and is not accompanied by changes in the morphology and viability of bacterial cells. The damage of the surface structures and the consequent destruction of Escherichia coli are observed upon contact with single-wall carbon nanotubes with low degrees of purification, which is presumably determined by the presence of technological impurities in CBNs. A significant enhancement of the affinity of C60 fullerenes functionalized by amine groups to the surface of model microorganisms, which also leads to the development of a bactericidal effect, is shown.
KeywordsAtomic Force Microscopy Single Wall Carbon Single Wall Carbon Nanotubes Atomic Force Microscopy Technique Outer Medium
Unable to display preview. Download preview PDF.
- 1.A. Ya. Vul’ and V. I. Sokolov, “Investigation of Nanocarbon in Russia: From Fullerenes to Nanotubes and Nanodiamonds,” Ross. Nanotekhnol. 2(3–4), 17–30 (2007).Google Scholar
- 7.E. A. Obraztsova, E. P. Lukashev, A. P. Zarubina, I. M. Parkhomenko, and I. V. Yaminsky, “Bactericidal Action of Single-Walled Carbon Nanotubes,” Vestn. Mosk. Univ., Ser. 3: Fiz., Astron., No. 3, 81–84 (2009) [Moscow Univ. Phys. Bull. 64 (3), 320–323 (2009)].Google Scholar
- 8.A. P. Zarubina, E. P. Lukashev, L. I. Deev, I. M. Parkhomenko, and A. B. Rubin, “Biotesting the Biological Effects of Single-Walled Carbon Nanotubes Using Bioluminescent Bacteria Test-System,” Ross. Nanotekhnol. 4(11–12), 152–155 (2009) [Nanotechnol. Russ. 4 (11–12), 871–875 (2009)].Google Scholar
- 12.V. S. Danilov, A. P. Zarubina, G. E. Eroshnikov, L. N. Solov’eva, F. V. Katashev, and G. B. Zavil’gel’skii, “Sensor Bioluminescent Systems Based on Lux-Operons of Different Types of Luminescent Bacteria,” Vestn. Mosk. Univ., Ser. 16: Biol., No. 3, 20–24 (2002).Google Scholar
- 16.A. Simon-Deckers, S. Loo, M. Mayne-L’hermite, N. Herlin-Boime, N. Menguy, C. Reynaud, B. Gouget, and M. Carriere, “Size-, Composition- and Shape-Dependent Toxicological Impact of Metal Oxide Nanoparticles and Carbon Nanotubes toward Bacteria,” Environ. Sci. Technol. 43(21), 8423–8429 (2009).CrossRefGoogle Scholar