Preparation of Tissue-Specific Matrix from Decellularized Porcine Cartilage


For full decellularization of cartilage tissue when preparing tissue-specific matrices of dense tissues from decellularized porcine articular cartilage, treatment with surfactant should be followed by treatment with supercritical fluids. Ethanol introduced as a polarity modifier leads to more complete cartilage decellularization, which is associated with a reduction of destructive changes in the extracellular matrix. The preserved morphology and active proliferation of mesenchymal stromal cells of human adipose tissue in the presence of the tissue-specific matrix of porcine cartilage indicate that the matrix is promising for use as a cell carrier for tissue engineering of cartilage.

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This study was partially supported by the Russian Foundation for Basic Research (grant no. 18-29-06012).

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Correspondence to E. A. Nemets.

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Translated by L. Smolina

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Nemets, E.A., Lazhko, A.E., Basok, Y.B. et al. Preparation of Tissue-Specific Matrix from Decellularized Porcine Cartilage. Russ. J. Phys. Chem. B 14, 1245–1251 (2020).

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  • Keywords: porcine cartilage
  • decellularization
  • surfactants
  • supercritical fluids
  • ethanol
  • histological studies
  • DNA
  • mesenchymal stromal cells