Preparation of Tissue-Specific Matrix from Decellularized Porcine Cartilage

Abstract

For full decellularization of cartilage tissue when preparing tissue-specific matrices of dense tissues from decellularized porcine articular cartilage, treatment with surfactant should be followed by treatment with supercritical fluids. Ethanol introduced as a polarity modifier leads to more complete cartilage decellularization, which is associated with a reduction of destructive changes in the extracellular matrix. The preserved morphology and active proliferation of mesenchymal stromal cells of human adipose tissue in the presence of the tissue-specific matrix of porcine cartilage indicate that the matrix is promising for use as a cell carrier for tissue engineering of cartilage.

This is a preview of subscription content, access via your institution.

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.

REFERENCES

  1. 1

    S. V. Got’e and S. M. Khomyakov, Vestn. Transplantol. Iskusstv. Organov 21 (3), 7 (2019).

    Google Scholar 

  2. 2

    B. Zylińska, P. Silmanowicz, A. Sobczyńska-Rak, L. Jarosz, and T. Szponder, In Vivo 32, 1289 (2018).

    Article  Google Scholar 

  3. 3

    V. I. Sevastianov, Y. B. Basok, A. M. Grigor’ev, L. A. Kirsanova, and V. N. Vasilets, Biomed. Eng. 51, 162 (2017).

    Article  Google Scholar 

  4. 4

    K. A. Dubey, C. V. Chaudhari, Y. K. Bhardwaj, and L. Varshney, Adv. Struct. Mater. 66, 1 (2017).

    Article  Google Scholar 

  5. 5

    P. M. Crapo, T. W. Gilbert, and S. F. Badylak, Biomaterials 32, 3233 (2011).

    CAS  Article  Google Scholar 

  6. 6

    N. Shirakigawa, H. Ijima, and T. Takei, J. Biosci. Bioeng. 114, 546 (2012).

    CAS  Article  Google Scholar 

  7. 7

    B. Oberwallner, A. Brodarac, Y.-H. Choi, T. Saric, P. Anić, L. Morawietz, and C. Stamm, J. Biomed. Mater. Res. A 102, 3263 (2014).

    Article  Google Scholar 

  8. 8

    T. W. Gilbert, S. Wognum, E. M. Joyce, D. O. Freytes, M. S. Sacks, and S. F. Badylak, Biomaterials 29, 4775 (2008).

    CAS  Article  Google Scholar 

  9. 9

    A. Gilpin and Y. Yong, Biomed. Res. Int. 2017, 13 (2017).

    Article  Google Scholar 

  10. 10

    B. Cox and A. Emiki, Nat. Protoc. 1, 1872 (2006).

    CAS  Article  Google Scholar 

  11. 11

    C. C. Xu, R. W. Chan, and N. Tirunagari, Tissue Eng. 13, 551 (2007).

    CAS  Article  Google Scholar 

  12. 12

    N. Patel, E. Solanki, R. Picciani, V. Cavett, J. A. Caldwell-Busby, and S. K. Bhattacharya, Proteomics 8, 1055 (2008).

    CAS  Article  Google Scholar 

  13. 13

    N. Shirakigawa and H. Ijima, Adv. Struct. Mater. 66, 185 (2017).

    Article  Google Scholar 

  14. 14

    Z. Wang, Z. Li, Z. Li, B. Wu, Y. Liu, and W. Wu, Acta Biomater. 81, 129 (2018).

    CAS  Article  Google Scholar 

  15. 15

    E. A. Nemets, L. A. Kirsanova, Yu. B. Basok, M. Yu. Shagidulin, E. A. Volkova, S. T. Metel’skii, and V. I. Sevast’yanov, Vestn. Transplantol. Iskusstv. Organ. 19 (4), 70 (2017).

    Google Scholar 

  16. 16

    S. H. Hung, C. H. Su, F. P. Lee, and H. Tseng, J. Voice 27, 289 (2013).

    Article  Google Scholar 

  17. 17

    GOST (State Standard) ISO 10993-1-2011: Medical products. Assessment of the biological effect of medical devices. Part 5. Cytotoxicity study: in vitro methods.

  18. 18

    J. Antons, M. G. Marascio, P. Aeberhard, G. Weissenberger, N. Hirt-Burri, L. A. Applegate, P. E. Bourban, and D. P. Pioletti, Eur. Cell. Mater. 36, 81 (2018).

    CAS  Article  Google Scholar 

  19. 19

    Y. Sun, L. Yan, S. Chen, and M. Pei, Acta Biomater. 74, 56 (2018).

    CAS  Article  Google Scholar 

Download references

Funding

This study was partially supported by the Russian Foundation for Basic Research (grant no. 18-29-06012).

Author information

Affiliations

Authors

Corresponding author

Correspondence to E. A. Nemets.

Additional information

Translated by L. Smolina

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Nemets, E.A., Lazhko, A.E., Basok, Y.B. et al. Preparation of Tissue-Specific Matrix from Decellularized Porcine Cartilage. Russ. J. Phys. Chem. B 14, 1245–1251 (2020). https://doi.org/10.1134/S1990793120080059

Download citation

  • Keywords: porcine cartilage
  • decellularization
  • surfactants
  • supercritical fluids
  • ethanol
  • histological studies
  • DNA
  • mesenchymal stromal cells