Russian Journal of Physical Chemistry B

, Volume 9, Issue 3, pp 454–460 | Cite as

Lux biosensors for antibiotic detection: The contribution from reactive oxygen species to the bactericidal activity of antibiotics

  • G. B. Zavil’gel’skii
  • V. Yu. Kotova
  • A. S. Mironov
Chemical Physics of Ecological Processes


Highly sensitive specific lux biosensors based on Escherichia coli bacteria have been designed for the detection of tetracycline, β-lactam, and quinolone antibiotics. The bacteria contain pTet′::lux, pAmpC′::lux, pColD′::lux hybrid plasmids in which the transcription of the luxCDABE reporter genes of Photorhabdus luminescens is performed under the control of the inducible promoter genes tetA, ampC, and cda, respectively. The basic characteristics of the lux biosensors (sensitivity threshold, response amplitude, and response time) have been determined. The contribution from reactive oxygen species to the bactericidal activity of quinolone antibiotics has been evaluated.


biosensor luciferase bioluminescence inducible promoter antibiotic reactive oxygen species 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    L. Galluzzi and M. Karp, Comb. Chem. High Thoroughput Screen 9, 501 (2006).CrossRefGoogle Scholar
  2. 2.
    J. H. Lee, R. J. Mitchell, B. C. Kim, et al., Biosens. Bioelectron. 21, 500 (1995).CrossRefGoogle Scholar
  3. 3.
    C. A. Vollmer and T. K. van Dyk, Adv. Microb. Physiol. 49, 131 (2004).CrossRefGoogle Scholar
  4. 4.
    M. Woutersen, S. Belkin, B. Brouwer, et al., Anal. Bioanal. Chem. 400, 914 (2011).CrossRefGoogle Scholar
  5. 5.
    G. B. Zavilgelsky, V. Yu. Kotova, and I. V. Manukhov, Khim. Fiz. 31, 15 (2012).Google Scholar
  6. 6.
    D. J. Dwyer, M. A. Kohanski, B. Hayele, and J. J. Collins, Mol. Syst. Biol. 3, 91 (2007).CrossRefGoogle Scholar
  7. 7.
    M. A. Kohanski, D. J. Dwyer, B. Hayele, C. A. Lawrence, and J. J. Collins, Cell 130, 797 (2007).CrossRefGoogle Scholar
  8. 8.
    D. J. Dwyer, M. A. Kohanski, and J. J. Collins, Curr. Opin. Microbiol. 12, 482 (2009).CrossRefGoogle Scholar
  9. 9.
    X. Wang, X. Zhao, M. Malik, and K. Drlica, J. Antimicrob. Chemother. 65, 520 (2010).CrossRefGoogle Scholar
  10. 10.
    M. A. Kohanski, D. J. Dwyer, and J. J. Collins, Nature Rev. Microbiol. 8, 423 (2010).CrossRefGoogle Scholar
  11. 11.
    K. Shatalin, E. Shatalina, A. Mironov, and E. Nudler, Science 334, 986 (2011).CrossRefGoogle Scholar
  12. 12.
    P. Belenky and J. J. Collins, Science 334, 915 (2011).CrossRefGoogle Scholar
  13. 13.
    J. J. Foti, B. Devadoss, J. A. Winkler, J. J. Collins, and G. C. Walker, Science 336, 315 (2012).CrossRefGoogle Scholar
  14. 14.
    Y. Liu and J. A. Imlay, Science 339, 1210 (2013).CrossRefGoogle Scholar
  15. 15.
    I. Keren, Y. Wu, J. Inocencio, L. R. Mulcahy, and K. Lewis, Science 339, 1213 (2013).CrossRefGoogle Scholar
  16. 16.
    V. Yu. Kotova, I. V. Manukhov, and G. B. Zavilgelsky, Appl. Biochem. Microbiol. 46, 781 (2010).CrossRefGoogle Scholar
  17. 17.
    A. Carlioz and D. Touati, EMBO J. 5, 623 (1986).Google Scholar
  18. 18.
    J. Sambrook and D. W. Russell, Molecular Cloning: A Laboratory Manual, 3rd ed. (Cold Spring Harbor Laboratory Press, New York, 2001).Google Scholar
  19. 19.
    S. Lindqvist, F. Lindberg, and S. Normark, J. Bacteriol. 171, 3746 (1989).Google Scholar
  20. 20.
    J. A. Imlay, S. M. Chin, and S. Linn, Science 240, 640 (1988).CrossRefGoogle Scholar
  21. 21.
    T. Nunoshiba, F. Obata, A. C. Boss, et al., J. Biol. Chem. 274, 34832 (1999).CrossRefGoogle Scholar
  22. 22.
    S. Park, X. You, and J. A. Imlay, Proc. Natl. Acad. Sci. USA 102, 9317 (2005).CrossRefGoogle Scholar
  23. 23.
    O. C. Kuo, T. Mashino, and I. Fridovich, J. Biol. Chem. 262, 4724 (1987).Google Scholar
  24. 24.
    P. R. Gardner and I. Fridovich, J. Biol. Chem. 266, 1478 (1991).Google Scholar
  25. 25.
    P. R. Gardner and I. Fridovich, J. Biol. Chem. 266, 19328 (1991).Google Scholar
  26. 26.
    S. I. Liochev and I. Fridovich, Proc. Natl. Acad. Sci. USA 89, 5892 (1992).CrossRefGoogle Scholar
  27. 27.
    D. H. Flint, J. F. Tuminello, and M. H. Emptage, J. Biol. Chem. 268, 22369 (1993).Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • G. B. Zavil’gel’skii
    • 1
  • V. Yu. Kotova
    • 1
  • A. S. Mironov
    • 1
  1. 1.State Research Institute of Genetics and Selection of Industrial MicroorganismsMoscowRussia

Personalised recommendations