Russian Journal of Physical Chemistry B

, Volume 9, Issue 3, pp 454–460 | Cite as

Lux biosensors for antibiotic detection: The contribution from reactive oxygen species to the bactericidal activity of antibiotics

  • G. B. Zavil’gel’skii
  • V. Yu. Kotova
  • A. S. Mironov
Chemical Physics of Ecological Processes

Abstract

Highly sensitive specific lux biosensors based on Escherichia coli bacteria have been designed for the detection of tetracycline, β-lactam, and quinolone antibiotics. The bacteria contain pTet′::lux, pAmpC′::lux, pColD′::lux hybrid plasmids in which the transcription of the luxCDABE reporter genes of Photorhabdus luminescens is performed under the control of the inducible promoter genes tetA, ampC, and cda, respectively. The basic characteristics of the lux biosensors (sensitivity threshold, response amplitude, and response time) have been determined. The contribution from reactive oxygen species to the bactericidal activity of quinolone antibiotics has been evaluated.

Keywords

biosensor luciferase bioluminescence inducible promoter antibiotic reactive oxygen species 

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • G. B. Zavil’gel’skii
    • 1
  • V. Yu. Kotova
    • 1
  • A. S. Mironov
    • 1
  1. 1.State Research Institute of Genetics and Selection of Industrial MicroorganismsMoscowRussia

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