Russian Journal of Physical Chemistry B

, Volume 7, Issue 3, pp 338–342 | Cite as

Small-angle X-ray scattering study of the incorporation of ICHPHAN antioxidant in liposomes

  • A. V. Krivandin
  • L. D. Fatkullina
  • O. V. Shatalova
  • A. N. Goloshchapov
  • E. B. Burlakova
Chemical Physics of Biological Processes

Abstract

The structure of multiplayer liposomes prepared from chloroform solutions of egg phosphatidylcholine and ICHPHAN-10-C-10 synthetic antioxidant at ICHPHAN-to-lipid mass ratios of 0, 2, 4, 5, 10, 15 and 20% was studied by small-angle X-ray scattering. ICHPHAN caused a decrease in the lipid membrane thickness and in the period of their stacking in liposomes. This effect was most pronounced at a ICHPHAN-to-lipid mass ratio in the dispersion of 5%. At higher ICHPHAN concentrations, a decline in the ordering of the membranes in the liposomes was observed, which is presumably explained by ICHPHAN microphase segregation in the lipid multilayer. EPR spin probe measurements showed no appreciable changes in the lipid membrane microviscosity in the presence of ICHPHAN. The results demonstrated the ability of ICHPHAN to incorporate into lipid membranes in considerable quantities. Liposomes containing ICHPHAN can be used for developing new effective drugs for treatment of neurodegenerative diseases.

Keywords

ICHPHAN antioxidant liposomes lipid membrane structure small-angle X-ray scattering EPR spin probes 

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • A. V. Krivandin
    • 1
  • L. D. Fatkullina
    • 1
  • O. V. Shatalova
    • 1
  • A. N. Goloshchapov
    • 1
  • E. B. Burlakova
    • 1
  1. 1.Emanuel Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussia

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