Russian Journal of Physical Chemistry B

, Volume 3, Issue 4, pp 652–655 | Cite as

Structural state of erythrocyte membranes in a human with Alzheimer’s disease

  • N. Yu. Gerasimov
  • A. N. Goloshchapov
  • E. B. Burlakova
Macrokinetics and Dynamics of Transport Processes


The structural state of erythrocyte membranes collected from patients with Alzheimer’s disease (AD) was studied. The structural state was characterized by the degree of hemolysis and the content of malonic dialdehyde (MDA), as measures of the peroxide oxidation of lipids (POL), and by the microviscosity of the lipid bilayer. The microviscosity of the membranes was measured by the EPR spin probe method, with 2,2,6,6-tetramethyl-4-capryloyl-oxypiperidine-1-oxyl (lipid probe) and 5,6-benzo-2,2,6,6-tetramethyl-1,2,3,4-tetrahydro-gamma-carboline-3-oxyl (protein probe). It was established that all patients with AD feature an increased fluidity of both the lipid and near-protein areas of erythrocyte membranes. Based on the POL intensity, three groups of patients were identified. The first group was characterized by an increased POL intensity. This group featured a high degree of hemolysis and a high MDA content both before and after incubation, with increases in both these parameters being observed upon incubation. The second and third groups were characterized by a low POL intensity. The second group exhibited a low degree of hemolysis and no increment upon incubation, with the MDA/PH ratio being much higher than unity. The third group was characterized by an increased degree of mechanical hemolysis and by decreases in the degree of homolysis and the MDA content upon incubation.


Homolysis Malonic Dialdehyde Protein Probe Lipid Probe Mechanical Hemolysis 
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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • N. Yu. Gerasimov
    • 1
  • A. N. Goloshchapov
    • 1
  • E. B. Burlakova
    • 1
  1. 1.Emanuel Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussia

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