Molecularly imprinted polymers (MIP) in electroanalysis of proteins

  • V. V. Shumyantseva
  • T. V. Bulko
  • I. H. Baychorov
  • A. I. Archakov


The review summarizes current knowledge on the main approaches used for creation of high affinity polymer analogs of antibodies (known as molecularly imprinted polymers, MIP) applicable for electroanalysis of functionally important proteins such as myoglobin, troponin T, albumin, ferritin, lysozyme, calmodulin. The main types of monomers for MIP preparation as well as methods convenient for analysis of MIP/protein interactions, such as surface plasmon resonance (SPR), nanogravimetry with the use of a quartz crystal resonator (QCM), spectral and electrochemical methods have been considered. Special attention is paid to experimental data on electrochemical registration of myoglobin by means of o-phenylenediaminebased MIP electrodes. It was shown that the imprinting factor calculated as a ratio of the myoglobin signal obtained after myoglobin insertion in MIP to the myoglobin signal obtained after myoglobin insertion in the polymer lacking the molecular template (NIP) is 2–4.


molecular imprinting molecularly imprinted polymers (MIP) nanomaterials electroanalysis electrochemical sensors MIP sensor proteins 

Abbreviations used


cyclic voltammetry


differential pulse voltammetry


electrochemical impedance spectroscopy


quartz crystal microbalance


surface plasmon resonance


square wave voltammetry


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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • V. V. Shumyantseva
    • 1
    • 2
  • T. V. Bulko
    • 1
    • 2
  • I. H. Baychorov
    • 3
  • A. I. Archakov
    • 2
  1. 1.IBMC-EcoBioPharmMoscowRussia
  2. 2.Institute of Biomedical ChemistryMoscowRussia
  3. 3.Polyclinics no. 4, State Enterprise no 2MoscowRussia

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