Manganese in atherogenesis: Detection, origin, and a role

  • A. P. Lozhkin
  • T. B. Biktagirov
  • V. A. Abdul’yanov
  • O. V. Gorshkov
  • E. V. Timonina
  • G. V. Mamin
  • S. B. Orlinskii
  • N. I. Silkin
  • V. M. Chernov
  • R. N. Khairullin
  • M. Kh. Salakhov
  • O. N. Ilinskaya
Article

Abstract

The role of transition metal ions in atherogenesis is controversial; they may be involved in hydroxyl radical generation and can also catalyze the reactive oxygen species neutralization reaction as cofactors of antioxidant enzymes. Using EPR spectroscopy, we revealed that 70% of aorta specimens with atherosclerotic lesions possessed superoxide dismutase activity, 100% of the specimens initiated Fenton reaction and demonstrated the presence of manganese paramagnetic centers. The sodA gene encoding manganese-dependent bacterial superoxide dismutase was not found in the samples of atherosclerotic plaques by PCR using degenerate primers. The data obtained indicate prospects of manganese analysis as a marker element in the express diagnostics of atherosclerosis.

Keywords

atherosclerosis superoxide dismutase manganese sodA gene 

Abbreviations used

LDL

low density lipoproteins

ROS

reactive oxygen species

SOD

superoxide dismutase

TMTH

N-(1-hydroxy-2,2,6,6,-tetramethylpiperidine-4-yl)-2-methylpropaneamide hydrochloride

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • A. P. Lozhkin
    • 1
  • T. B. Biktagirov
    • 1
  • V. A. Abdul’yanov
    • 3
  • O. V. Gorshkov
    • 2
  • E. V. Timonina
    • 1
  • G. V. Mamin
    • 1
  • S. B. Orlinskii
    • 1
  • N. I. Silkin
    • 1
  • V. M. Chernov
    • 2
  • R. N. Khairullin
    • 3
  • M. Kh. Salakhov
    • 1
  • O. N. Ilinskaya
    • 1
  1. 1.Kazan State UniversityKazanRussia
  2. 2.Kazan Institute of Biochemistry and BiophysicsKazan Scientific Center of Russian Academy of SciencesKazanRussia
  3. 3.Interregional Clinical Diagnostic CenterKazanRussia

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