A mechanism of the toxicity of artificial ribonucleases for human cancer cells
The ability of artificial ribonucleases, low molecular weight compounds exhibiting RNA cleavage in vitro, to cause human cancer cell death in a concentration-dependent manner has been studied. The cytotoxic effect of artificial ribonucleases on cells appeared at rather low concentrations of these compounds (10−5 M). The study of mechanisms of the cytotoxic effect has shown that in addition to ribonuclease activity these compounds exhibit membranotropic activity. This activity allows the compounds to penetrate effectively inside cells. The cytotoxic effect of artificial ribonucleases involves damage of cell membrane, detachment of plasmalemma and impairments of its macromolecular organization. However, in the case of shortterm exposure to these compounds, cells survive even with damaged membrane.
Key wordsartificial ribonucleases citotoxicity membranotropic activity antineoplastic drugs
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