Tonoplast Rafts Stimulate DNA Import into the Mitochondria of Potato Tubers (Solanum tuberosum)


The effects of tonoplast and rafts isolated from vacuolar membranes on the import of DNA into mitochondria of potato tubers (Solanum tuberosum) were studied. It was shown that the addition of rafts isolated from the tonoplast to mitochondria increased 8-fold the activity of the DNA import into mitochondria as compared to the import in the untreated mitochondria.

This is a preview of subscription content, access via your institution.

Fig. 1.


  1. 1

    Stacchiotti A., Favero G., Lavezza A., Garcia-Gomez R., Monsalve M., Rezzani R. 2018. Perspective: Mitochondria-ER contacts in metabolic cellular stress assessed by microscopy. Cells. 8, 1–9.

    Article  Google Scholar 

  2. 2

    Fujimotoat M., Hayashi T., Su T.P. 2012. The role of cholesterol in the association of endoplasmic reticulum membranes with mitochondria. Biochem. Biophys. Res. Commun. 417, 635–639.

    Article  Google Scholar 

  3. 3

    Janikiewicz J., Szymanski J., Malinska D., Patalas-Krawczyk P., Michalska B., Duszynski J., Giorfi C., Bonora M., Dobrzyn A., Wieckowski M.R. 2018. Mitochondria-associated membranes in aging and senescence: Structure, function, and dynamics. Cell Death Disease. 9, 1–12.

    CAS  Article  Google Scholar 

  4. 4

    Camões F., Bonekamp N.A., Delille H.K., Schrader M. 2009. Organelle dynamics and dysfunction: A closer link between peroxisomes and mitochondria. J. Inherit. Metab. Dis. 32, 163–180.

    Article  Google Scholar 

  5. 5

    Shai N., Schuldiner M., Zalckvar E. 2016. No peroxisome is an island – Peroxisome contact sites. Biochim. Biophys. Acta. 1863, 1061–1069.

    CAS  Article  Google Scholar 

  6. 6

    Honscher C., Mari M., Auffarth K., Bohnert M., Griffith J., Geerts W., van de Laan M., Cabrera M., Reggiori F., Ungermann C. 2014. Cellular metabolism regulates contact sites between vacuoles and mitochondria. Dev. Cell.30 (1), 86–94.

    Article  Google Scholar 

  7. 7

    Ilacqua N., Sanchez-Alvarez M., Bachmann M., Costiniti V., Del Pozo M.A., Giacomello M. 2017. Protein localization at mitochondria-ER contact sites in basal and stress conditions. Front. Cell Develop. Biol. 5, 1–14.

    Article  Google Scholar 

  8. 8

    Pike L.J. 2006. Rafts defined: A report on the Keystone Symposium on lipid rafts and cell function. J. Lipid Res.47 (7), 1597–1598.

    CAS  Article  Google Scholar 

  9. 9

    Simons K., Sampaio J.L. 2011. Membrane organization and lipid rafts. Cold Spring Harb. Perspect. Biol.3 (10), 1–17.

    Article  Google Scholar 

  10. 10

    Vereshchagina N.A., Konstantinov Yu.M., Kamensky P.A., Mazunin I.O. 2018. Import of proteins and nucleic acids into mitochondria. Biokhimia (Rus.). 83 (6), 816–838.

    Google Scholar 

  11. 11

    Weber-Lotfi F., Koulintchenko M.V., Ibrahim N., Hammann P., Mileshina D.V., Konstantinov Yu.M., Dietrich A. 2015. Nucleic acid import into mitochondria: New insights into the translocation pathways. Biochim. Biophys. Acta. 1853, 3165–3181.

    CAS  Article  Google Scholar 

  12. 12

    Koulintchenko M., Konstantinov Y., Dietrich A. 2003. Plant mitochondria actively import DNA via the permeability transition pore complex. EMBO J.22 (6), 1245–1254.

    CAS  Article  Google Scholar 

  13. 13

    Klimenko E.S., Shmakov V.V., Bolotova T.A., Subota I.Yu., Tarasenko V.I., Kulinchenko M.V., Konstantinov Yu.M. 2018. Study of the role of interaction between mitochondrial and endoplasmic reticulum membranes in DNA import. Proceedings of the II all-Russian scientific conference “Mechanisms of regulation of eukaryotic cell organelle functions”. Irkutsk, p. 49–51.

  14. 14

    Neuburger M. 1982. Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll. Arch. Biochem. Biophys.217 (1), 312–323.

    CAS  Article  Google Scholar 

  15. 15

    Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1–2), 248–254.

    CAS  Article  Google Scholar 

  16. 16

    Klimenko E.S., Mileyko V.A., Morozkin E.S., Laktionov P.P., Konstantinov Yu.M. 2011. Characterization of import and export of DNA in mitochondria of potato (Solanum tuberosum) using quantitative PCR. Biol. Membrany (Rus.). 28 (3), 199–205.

    CAS  Google Scholar 

  17. 17

    Salyaev R.K., Kuzevanov V.Ya., Khaptagaev S.B., Kopytchuk V.N. 1981. Isolation and purification of vacuoles and vacuolar membranes from plant cells. Fiziologiya rasteniy (Rus.). 28 (6), 1295–1306.

  18. 18

    Ozolina N.V., Nesterkina I.S., Kolesnikova E.V., Salyaev R.K., Nurminsky V.N., Rakevich A.L., Martynovich E.F., Chernyshov M.Y. 2013. Tonoplast of Beta vulgaris L. contains detergent-resistant membrane microdomains. Planta.237 (3), 859–871.

    CAS  Article  Google Scholar 

  19. 19

    Konstantinov Yu.M., Dietrich A., Weber-Lotfi F., Ibrahim N., Klimenko E.S., Tarasenko V.I., Bolotova T.A., Kulinchenko M.V. 2016. Import of DNA into mitochondria. Biokhimiya (Rus.). 81, 1307–1321.

    Google Scholar 

  20. 20

    Flis V.V., Daum G. 2013. Lipid transport between the endoplasmic reticulum and mitochondria. In: The endoplasmic reticulum. A subject collection from Cold Spring Harbor Perspectives in Biology. Eds. Ferro-Novick S., Rapoport T.A., Schekman R. Cold Spring Harbor, New York, USA: Cold Spring Harbor Laboratory Press, p. 109–130.

    Google Scholar 

  21. 21

    McEnery M.W., Snowman A.M., Trifiletti R.R., Snyder S.H. 1992. Isolation of the mitochondrial benzo-diazepine receptor: Association with the voltage-dependent anion channel and adenine nucleotide carrier. Proc. Natl. Acad. Sci. USA.89, 3170–3174.

    CAS  Article  Google Scholar 

  22. 22

    Zorov D.B. 1996. Mitochondrial transport of nucleic acids. Involvement of the benzodiazepine receptor. Biochemistry (Moscow).61, 939–946.

    Google Scholar 

  23. 23

    Yoshida K., Ohnishi M., Fukao Y., Okazaki Y., Fujiwara M., Song C., Nakanishi Y., Saito K., Shimmen T., Suzaki T., Hayashi F., Fukaki H., Maeshima M., Mimura T. 2013. Studies on vacuolar membrane microdomains isolated from Arabidopsis suspension-cultured cells: Local distribution of vacuolar membrane proteins. Plant Cell Physiol.54 (10), 1571–1584.

    CAS  Article  Google Scholar 

Download references


We thank A.V. Panov for his interest and valuable help in preparing of the English version of the paper. The work was partially supported by the grant of the RF President (project no. MK-6666.2020.11) and by the Russian Foundation for Basic Research (project nos. 18-04-00603 and 19-04-00013). Experiments were conducted using the equipment of the Central Research Center Bioanalytics of the Siberian Institute of Plant Physiology and Biochemistry SB RAS (Irkutsk).

Author information



Corresponding authors

Correspondence to N. V. Ozolina or Yu. M. Konstantinov.

Ethics declarations


The authors declare that they have no conflict of interest.

This article does not contain any studies involving animals or human participants performed by any of the authors.


E. S. Klimenko. and I. S. Nesterkina equally contributed to this work.

Additional information

Translated by E. Puchkov

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Klimenko, E.S., Nesterkina, I.S., Ozolina, N.V. et al. Tonoplast Rafts Stimulate DNA Import into the Mitochondria of Potato Tubers (Solanum tuberosum). Biochem. Moscow Suppl. Ser. A 14, 351–354 (2020).

Download citation


  • Solanum tuberosum
  • mitochondria
  • DNA
  • import
  • tonoplast
  • lipid rafts