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The effects of inhibitors and magnesium ions on the activity of the thermostable extracellular cAMP-Specific phosphodiesterase of Physarum polycephalum plasmodium

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Plasmodium of myxomycete Physarum polycephalum produces cyclic nucleotide phosphodiesterase (PDE). The extracellular PDE is cAMP-specific and highly thermostable. This study demonstrates that the extracellular PDE of Ph. polycephalum is weakly inhibited by caffeine, isobutylmethylxantine and theophiline (type I mammalian PDE nonspecific inhibitors), dipyridamole (mammalian PDE5, PDE6, PDE8 and PDE10 inhibitors), and erythro-9-[3-(2-hydroxynonyl)]-adenine (mammalian PDE2 inhibitor). The enzyme does not require Mg2+ for the activity. The results show that the Ph. polycephalum extracellular PDE differs from class I PDEs, represented by mammalian PDE1-PDE11, and, most likely, belongs to a poorly investigated class II PDEs.

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Abbreviations

PDE:

cyclic nucleotide phosphodiesterase

EHNA:

erythro-9-[3-(2-hydroxynonyl)]-adenine, mammalian PDE2 inhibitor

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    A. R. Nezvetsky, T. G. Orlova, S. I. Beylina & N. Ya. Orlov

Authors
  1. A. R. Nezvetsky
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  2. T. G. Orlova
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  3. S. I. Beylina
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  4. N. Ya. Orlov
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Correspondence to N. Ya. Orlov.

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Original Russian Text © A.R. Nezvetsky, T.G. Orlova, S.I. Beylina, N.Ya. Orlov, 2011, published in Biologicheskie Membrany, 2011, Vol. 28, No. 6, pp. 541–546.

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Nezvetsky, A.R., Orlova, T.G., Beylina, S.I. et al. The effects of inhibitors and magnesium ions on the activity of the thermostable extracellular cAMP-Specific phosphodiesterase of Physarum polycephalum plasmodium. Biochem. Moscow Suppl. Ser. A 6, 100–104 (2012). https://doi.org/10.1134/S1990747811060109

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  • DOI: https://doi.org/10.1134/S1990747811060109

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