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The priming effect of halogenated phospholipids on the functional responses of human neutrophils

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Halogenated lipids formed in the reactions with myeloperoxidase (MPO)-derived species may contribute to the regulation of the functional activity of cells. In the present study we have investigated the effects of chloro- and bromohydrins formed in the HOCl and HOBr reactions, respectively, with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) on three different functional responses of human neutrophils: H2O2 generation, degranulation (MPO exocytosis), and cell aggregation. It was shown that POPC chloro- and bromohydrins (POPC-Cl and POPC-Br) induced the priming of neutrophils, resulting in significant upregulation of cell responses to neutrophil stimulators such as N-formyl-Met-Leu-Phe and lectin from Solanum tuberosum. The stimulating effects of POPC-Cl and POPC-Br were observed at low micromolar concentrations (liposomal concentration of POPC, 0.5–5 μM; the content of POPC-Cl or POPC-Br, 38 ± 3% of total lipids) after a short exposure (about 5 min) of the neutrophils to POPC-Cl or POPC-Br. These results suggest that halogenated lipids formed in vivo via MPO-dependent reactions may be considered as a new class of biologically active substances that are potentially able to contribute to the priming of myeloid cells in the sites of inflammation and serve as inflammatory response modulators.

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Abbreviations

ROS:

reactive oxygen species

LDL:

low-density lipoproteins

HOCl-LDL:

HOCl-modified LDL

MPO:

myeloperoxidase

fMLF:

N-formyl-Met-Leu-Phe

GlcNAc:

N-acetyl-D-glucosamine

MALDI-TOF:

(matrix assisted laser desorption/ionisation time-of-flight), mass spectrometry

POPC:

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine

LS-POPC:

liposomes formed of POPC

POPC-Cl:

POPC chlorohydrins

POPC-Br:

POPC bromohydrins

STA:

lectin of potato tubers (Solanum tuberosum)

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Authors and Affiliations

  1. Department of Biophysics, Belarussian State University, prosp. Nezavisimosti, 4, Minsk, 220030, Belarus

    I. V. Gorudko, A. V. Mukhortova & S. N. Cherenkevich

  2. Research Institute of Physico-Chemical Medicine, ul. Malaya Pirogovskaya, 1a, Moscow, 119992, Russia

    T. V. Vakhrusheva, V. I. Sergienko & O. M. Panasenko

  3. Department of Biology, University of Western Ontario, London, Canada, N6A 5B7

    A. V. Timoshenko

Authors
  1. I. V. Gorudko
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  2. T. V. Vakhrusheva
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  3. A. V. Mukhortova
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  4. S. N. Cherenkevich
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  5. A. V. Timoshenko
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  6. V. I. Sergienko
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  7. O. M. Panasenko
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Correspondence to I. V. Gorudko.

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Original Russian Text © I.V. Gorudko, T.V. Vakhrusheva, A.V. Mukhortova, S.N. Cherenkevich, A.V. Timoshenko, V.I. Sergienko, O.M. Panasenko, 2010, published in Biologicheskie Membrany, 2010, Vol. 27, No. 4, pp. 314–324.

The article was translated by the authors.

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Gorudko, I.V., Vakhrusheva, T.V., Mukhortova, A.V. et al. The priming effect of halogenated phospholipids on the functional responses of human neutrophils. Biochem. Moscow Suppl. Ser. A 4, 262–271 (2010). https://doi.org/10.1134/S1990747810030037

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  • DOI: https://doi.org/10.1134/S1990747810030037

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