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Fluorimetric comparison of protomitochondria and mitochondria

Article

Abstract

Protomitochondria (PRM) are intracellular mitochondrial germ organelles, precursors of mitochondria in specialized cells of animals. PRM have been isolated from rat liver by centrifugation and filtration through Millipore filters with pore diameters of 0.1 to 0.45 μm and characterized by fluorescence-based assay. PRM, having the volume many times smaller than that of light mitochondria (LM), did not differ much from the latter in protein, lipid and DNA composition, membrane charge, and some other parameters. At the same time, the activity of some enzymes of the respiratory chain (NADH dehydrogenase, succinate dehydrogenase) in PRM was even higher than that in mature mitochondria. The results obtained are important for understanding the processes of mitochondrial biogenesis in specialized cells of mammals.

Keywords

NADH Pyrene Rotenone Succinate Dehydrogenase Supplement Series 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  1. 1.Institute of Cell BiophysicsRussian Academy of SciencesPushchino, Moscow oblastRussia

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