Fluorimetric comparison of protomitochondria and mitochondria



Protomitochondria (PRM) are intracellular mitochondrial germ organelles, precursors of mitochondria in specialized cells of animals. PRM have been isolated from rat liver by centrifugation and filtration through Millipore filters with pore diameters of 0.1 to 0.45 μm and characterized by fluorescence-based assay. PRM, having the volume many times smaller than that of light mitochondria (LM), did not differ much from the latter in protein, lipid and DNA composition, membrane charge, and some other parameters. At the same time, the activity of some enzymes of the respiratory chain (NADH dehydrogenase, succinate dehydrogenase) in PRM was even higher than that in mature mitochondria. The results obtained are important for understanding the processes of mitochondrial biogenesis in specialized cells of mammals.


NADH Pyrene Rotenone Succinate Dehydrogenase Supplement Series 
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© MAIK Nauka 2008

Authors and Affiliations

  1. 1.Institute of Cell BiophysicsRussian Academy of SciencesPushchino, Moscow oblastRussia

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