Organophosphorous plant growth regulator Melaphen: Resistance of plant and animal cells to stress factors

  • I. V. Zhigacheva
  • E. B. Burlakova
  • A. G. Shugaev
  • I. P. Generozova
  • S. G. Fattakhov
  • A. I. Konovalov


The addition of the organophosphorous plant growth regulator Melaphen (4 × 10−12 M) to the incubation medium increases the maximum rate of oxidation of NAD-dependent substrates in rat liver and sugar beet root mitochondria. In addition, Melaphen stimulates electron transport during oxidation of succinate by rat liver mitochondria, but has no effect on the rate of this substrate oxidation in sugar beet root mitochondria. In storage organs of plants, the rate of oxidation of NAD-dependent substrates by mitochondria is relatively low. By stimulating the activity of NAD-dependent dehydrogenases, Melaphen stimulates energy metabolism in the cells and manifests adaptogenic activity by accelerating the germination of seeds. Melaphen does not influence the fluorescence of lipid peroxidation (LPO) products in mitochondria non-exposed to stress, but decreases 1.5–2 fold the LPO fluorescence in rat liver mitochondria exposed to cold stress and artificially “aged” sugar beet root mitochondria. Besides, Melaphen increases the rate of electron transport in a terminal site of respiratory chains of plant and animal mitochondria and decreases LPO. The data obtained testify to antistress activity of Melaphen.


Cold Stress Supplement Series Phosphinic Acid Sugar Beet Root Animal Mitochondrion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Chirkova, T.V., Cellular Membranes and Resistance of Plants to Stress, Sorosovskii Obrazovatel’nyi Zhurnal (Rus.), 1997, no. 9, pp. 12–17.Google Scholar
  2. 2.
    Taran, N.Yu., Changes in Adaptation of Lipid Components of Chloroplast Membranes Induced by Environmental Factors, Ukrainskii Biokhimicheskii Zhurnal (Ukr.), 2000, vol. 72, no. 1, pp. 21–31.Google Scholar
  3. 3.
    Baraboi, V.A., Stress Mechanisms and Lipid Peroxidation, Uspekhi Sovremennoi Biologii (Rus.), 1991, vol. 11, no. 6, pp. 923–932.Google Scholar
  4. 4.
    Kurganova, L.N., Lipid Peroxidation As a Component of Fast Response to Stress, Sorosovskii Obrazovatel’nyi Zhurnal (Rus.), 2001, no. 6, pp. 76–78.Google Scholar
  5. 5.
    Zhigacheva, I.V., Fatkullina, L.D., Shugaev, A.G., and Goloshchapov, A.N., Effects of Melaphen on Structural Characteristics of Biological Membranes, Proceedings of All-Russian Conference-Workshop “Studies and Perspectives of Plant Growth Regulator Melaphen in Agriculture and Biotechnology,” Kazan’, October 12–14, 2006, pp. 69–75.Google Scholar
  6. 6.
    Tukei, G., Regulyatory rosta rastenii v sel’skom khozyaistve (Plant Growth Regulators in Agriculture), Moscow: Mir, 1958.Google Scholar
  7. 7.
    Kefeli, V.I., Rost rastenii (Plant Growth), Moscow: Nauka, 1973.Google Scholar
  8. 8.
    Burkhanova, E.A., Fedina, A.B., Baskakov, Yu.A., and Kulaeva, O.N., Comparative Study of 6-Benzylaminopurine, Thiazurone, and Cartoline Effects on the Growth of Intact Pumpkin Germs, Fiziologiya Rastenii (Rus.), 1984, vol. 31, pp. 13–19.Google Scholar
  9. 9.
    Chalova, L.I. and Ozeretskovskaya, O.L., Biological Inducers of Protective Responses in Plants and Pathways to Their Practical Application, in Biokhimiya immuniteta, pokoya, stareniya rastenii (Biochemistry of Plant Immunity, Quiescent State, and Ageing), Berezin, I.V., Ed., Moscow: Nauka, 1984, pp. 41–57.Google Scholar
  10. 10.
    Korol’, V.V., Kirillova, I.G., and Puzina, T.I., Changes in the Hormonal Balance and Physiological Processes in the Potato Plant after Treatment with Growth Regulators and Microelements, Vestnik Bashkirskogo Universiteta (Rus.), 2001, no. 2, pp. 84–86.Google Scholar
  11. 11.
    Morohashi, Y., Effect of Benzyladenine on the Development of Mitochondrial Activities in Imbibed Black Gram Cotyledons, J. Plant Physiol., 1984, vol. 116, pp. 235–240.Google Scholar
  12. 12.
    Thomas, J.C. and Bohnert, H.J., Salt Stress Precipitation and Plant Growth Regulators in the Halophyte Mesembryanthemum crystallinum, Plant Physiol., 1993, vol. 103, pp. 1299–1304.PubMedGoogle Scholar
  13. 13.
    Fattakhov, S.G., Reznik, V.S., and Konovalov, A.I., The Melaminic Salt of Bis(Oxymethyl)Phosphinic Acid (MELAPHEN) As a Growth Regulator of New-Generation Plants, Proceedings of the 13th International Conference on Chemistry of Phosphorus Compounds, St. Petersburg, 2002, p. 80.Google Scholar
  14. 14.
    Kostin, V.I., Kostin, O.V., and Isaichev, V.A., The Use of Melaphen in Cropping, Proceedings of All-Russian Conference-Workshop “Studies and Perspectives of Plant Growth Regulator Melaphen in Agriculture and Biotechnology,” Kazan, 2006, pp. 27–37.Google Scholar
  15. 15.
    Fattakhov, S.G., Loseva, N.L., Konovalov, A.I., Reznik, V.S., Alyabyev, A.Yu., Gordon, L.Kh., and Tribunskikh, V.I., Effects of Melaphen on the Growth and Energy-Dependent Processes in Plant Cells, Doklady Akademii Nauk (Rus.), 2004, vol. 394, pp. 127–129.Google Scholar
  16. 16.
    Dauce, R., Mitochondria in Higher Plants: Structure, Function, and Biogenesis, Quarterly Rev. Biol., 1986, vol. 61, no. 2, pp. 260–261.Google Scholar
  17. 17.
    Kulinsky, V.I., Reactive Oxygen Species and Oxidative Modification of Molecules: Benefits, Detriments, and Protection, Sorosovskii Obrazovatel’nyi Zhurnal (Rus.), 1999, no. 2, pp. 2–7.Google Scholar
  18. 18.
    Shugaev, A.G. and Vyskrebentseva, E.I., Isolation of Intact Mitochondria from Sugar Beet Roots, Fiziologiya Rastenii (Rus.), 1982, vol. 29, pp. 799–803.Google Scholar
  19. 19.
    Zhigacheva, I.V., Kaplan, E.Ya., Pakhomov, V.Yu., Rozantseva, T.V., Khristianovich, D.S., and Burlakova, E.B., The Drug “Anphen” and the Energy Status of the Liver, Doklady Akademii Nauk (Rus.), 1995, vol. 340, no. 4, pp. 547–550.Google Scholar
  20. 20.
    Fletcher, B.I., Dillard, C.D., and Tappel, A.L., Measurement of Fluorescent Lipid Peroxidation Products in Biological Systems and Tissues, Anal. Biochem., 1973, vol. 52, pp. 1–9.PubMedCrossRefGoogle Scholar
  21. 21.
    Shugaev, A.G. and Vyskrebentseva, E.I., Effects of Rotenone and Exogenous NAD on Malate Oxidation by Mitochondria Isolated from Sugar Beet Roots at Different Steps of Plant Ontogenesis, Fiziologiya Rastenii (Rus.), 1985, vol. 32, pp. 259–267.Google Scholar
  22. 22.
    Kozlova, R.Yu. and Vinter, V.G., Melaphen As a Regulator of Synthesis of Pharmaceutically Important Alkaloids Synthesized by Biotechnological Methods, Proceedings of All-Russian Conference-Workshop “Studies and Perspectives of Plant Growth Regulator Melaphen in Agriculture and Biotechnology,” Kazan, October 12–14, 2006, pp. 102–114.Google Scholar
  23. 23.
    Savina, T.A. and Tsybul’ko, N.S., The use of Melaphen for Stimulation of Productivity of Cultured Cells in Vitro, Proceedings of All-Russian Conference-Workshop “Studies and Perspectives of Plant Growth Regulator Melaphen in Agriculture and Biotechnology,” Kazan, Shkola, October 12–14, 2006, pp. 138–143.Google Scholar
  24. 24.
    Sugaev, A.G., Some pecularities of Structural Organization and Oxidative Activity of the Respiration Chain of Plant Mitochondria, Uspekhi Sovremennoi Biologii, 1991, vol. 111, no. 2, pp. 178–191.Google Scholar

Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • I. V. Zhigacheva
    • 1
  • E. B. Burlakova
    • 1
  • A. G. Shugaev
    • 2
  • I. P. Generozova
    • 1
  • S. G. Fattakhov
    • 3
  • A. I. Konovalov
    • 3
  1. 1.Emmanuel Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussia
  2. 2.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  3. 3.Arbuzov Institute of Organic and Physical Chemistry, Kazan Research CenterRussian Academy of SciencesKazanRussia

Personalised recommendations