Cell and Tissue Biology

, Volume 12, Issue 2, pp 146–152 | Cite as

Establishment of the HeLa Cell Line with Stable Expression of CD63 Exosome Marker Fused with Fluorescent Protein TagRFP and HTBH Tag

  • V. A. Kulichkova
  • A. V. Selenina
  • A. N. Tomilin
  • A. S. Tsimokha


Exosomes are small microvesicles released into the cellular environment by different types of cells. These vesicles have been found in the blood serum and in other extracellular fluids of body. There are a number of different proteins, mRNA and microRNA, in these exosomes. Exosomes take part in cellular communication, excretion of proteins, immune response, and are also involved in development of some neurodegenerative diseases and cancer. The mechanism through which they get in and out of cells is not clear. To address this issue, we have generated a stable HeLa cell line expressing exosomal marker CD63 fused with a tagRFP and HTBH tag. These and other cells harboring the CD63-tagRFP-HTBH structure constitute a valuable tool that should allow real-time observations of exosomal transport


red fluorescence protein exosome tetraspanin CD63 



polyacrylamide gel


polymerase chain reaction


modified red fluorescent protein


complex polypeptide with two sequences of six histidine (H) specific site for TEV-protease cleavage (T) and site for biotinylation in vitro (B)


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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • V. A. Kulichkova
    • 1
  • A. V. Selenina
    • 1
  • A. N. Tomilin
    • 1
    • 2
  • A. S. Tsimokha
    • 1
  1. 1.Institute of CytologyRussian Academy of SciencesSt. PetersburgRussia
  2. 2.Institute of Translational BiomedicineSt. Petersburg State UniversitySt. PetersburgRussia

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