Studies of the expression of subunits α2 and β1 of Na+/K+-ATPase, α1S (L-type) Ca2+-channel, and SERCA 1/2/3 of Ca2+-ATPase of phasic and postural rat muscles in a model of hypogravity using the method of fluorescent microscopy
Using fluorescent microscopy, we found decreased expression of the β1 subunit of Na+/K+-ATPase and subunits of Ca2+-ATPase, increased expression of the α1S subunit of the L-type Ca2+-channel, and no changes in the expression of the α2 subunit of Na+/K+-ATPase in rat postural muscle under the conditions of modeled hypogravity. In the phasic muscle, we observed decreased expression of the β1 subunit, which was similar to that found in the postural muscle, whereas the other studied parameters remained without alterations. However, a decrease in the fluorescence intensity of the β1 subunit was insignificant due to a high variability of data. Thus, hypogravity negatively influenced primarily those skeletal muscles that are responsible for static load.
KeywordsNa+/K+-ATPase Ca2+-ATPase L-type Ca2+-channel hypogravity skeletal muscle
- m. EDL
extensor digitorum longus muscle
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- Genin, A.M., Il’in, A.E., Kaplanskiĭ, A.S., Kasatkina, T.B., Kuznetsova, K.A., Pestov, I.D., and Smirnova, T.A., Bioethics of research on humans and animals in aviation, space and marine medicine, Aviakosm. Ekol. Med., 2001, vol. 35, no. 4, pp. 14–20.Google Scholar
- Kravtsova, V.V., Ogneva, I.V., Altaeva, E.G., Razgovorova, I.A., Tiapkina, O.V., Nikol’skiĭ, E.E., Shenkman, B.S., and Krivoĭ, I.I., Electrogenic activity of Na-KATPase and calcium ions in m. soleus fibers of rats and Mongolian gerbil during simulation of gravitational unloading, Aviakosm. Ekol. Med., 2010, vol. 44, no. 2, pp. 35–44.Google Scholar
- Krivoi, I.I., Kravtsova, V.V., Altaeva, E.G., Kubasov, I.V., Prokof'ev, A.V., Drabkina, T.M., Nikol’sky, E.E., and Shenkman, B.S., Decrease in the electrogenic contribution of Na, K-ATPase and resting membrane potential as a possible mechanism of Ca2+ accumulation in rat soleus muscle in a short-term gravity unloading, Biophysics (Moscow), 2008, vol. 53, no. 6, pp. 586–592.CrossRefGoogle Scholar
- McDonough, A.A., Geering, K., and Farley, R.A., The sodium pump needs its beta subunit, FASEB J., 2002, vol. 4, pp. 1598–1605.Google Scholar
- Mukhina, A.M., Altaeva, E.G., Nemirovskaia, T.L., and Shenkman, B.S., The role of L-type calcium channels in the accumulation of Ca2+ in soleus muscle fibers in the rat and changes in the ratio of myosin and SERCA isoforms in conditions of gravitational unloading, Ross. Fiziol. Zh. Im. I.M. Sechenova, 2006, vol. 92, no. 11, pp. 1285, no. 1295.PubMedGoogle Scholar
- Tyapkina, O.V., Nurullin, L.F., Petrov, K.A., and Volkov, E.M., The effect of modelling of hypogravity on postsynaptic acetylcholine receptors and activity of acetylcholinesterase in neuromuscular synapses of fast and slow muscles of rat, Cell Tissue Biol., 2014a, vol 8, no. 6, pp. 499–503.Google Scholar