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Cell and Tissue Biology

, Volume 9, Issue 4, pp 261–264 | Cite as

DNA methylation level and telomere length as a basis for modeling of the biological aging clock

  • A. L. Runov
  • M. S. Vonsky
  • V. M. Mikhelson
Article
  • 85 Downloads

Abstract

Aging is a process defined by a variety of both external and internal factors. A person’s biological age determines the extent of bodily deterioration and risk of age-related diseases. Various parameters, including average telomere length and level of DNA methylation, are considered indicators of biological age. We proposed to combine these parameters to create a model suitable to assess human biological age. Application of qPCR for determination of telomere length and MS-HRM for methylation analysis will help to determine parameters of interest quickly and requires a limited set of equipment.

Keywords

aging biological clock DNA methylation telomere 

Abbreviation

PCR

polymerase chain reaction

RT-PCR

real-time PCR

HRM

high-resolution DNA melting

MALDI-TOF MS

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

MS-HRM

methylation sensitive high-resolution melting

NGS

next-generation sequencing

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • A. L. Runov
    • 1
    • 2
  • M. S. Vonsky
    • 1
    • 2
  • V. M. Mikhelson
    • 1
  1. 1.Institure of CytologyRussian Academy of SciencesSt. PetersburgRussia
  2. 2.Mendeleev Institute of MetrologySt. PetersburgRussia

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