Cell and Tissue Biology

, Volume 8, Issue 6, pp 499–503 | Cite as

The effect of modelling of hypogravity on postsynaptic acetylcholine receptors and activity of acetylcholinesterase in neuromuscular synapses of fast and slow muscles of rat

  • O. V. Tyapkina
  • L. F. Nurullin
  • K. A. Petrov
  • E. M. Volkov
Article
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Abstract

Rats were subjected to antiorthostatic hindlimb unloading for 35 days. Using immunofluorescent techniques, we found increased intensity of fluorescence and decreased area of staining of acetylcholine receptors and the increased intensity and area of staining of acetylcholinesterase in neuromuscular synapses of “fast” and “slow” muscles. Changes in the ratio of the number of acetylcholine receptors on the postsynaptic membrane and acetylcholinesterase and the alterations in their spatial location relative to each other in neuromuscular synapses of “fast” and “slow” muscles were also observed. These modifications are in accordance with the electrophysiological data on the decreased amplitudes of miniature end plate currents in both muscles. They were accompanied by a decrease in the volume of muscle fibers. Antiorthostatic unloading, a model of hypogravity, resulted in increased functional activity of acetylcholinesterase associated with decreased area of the postsynaptic membrane occupied by acetylcholine receptors, which led to a decrease in the amplitude of postsynaptic excitatory potentials and thus, to the decreased reliability threshold of excitation transmission from a nerve to a muscle.

Keywords

hypogravity modeling “fast” and “slow” muscles acetylcholinesterase acetylcholine receptors 

Abbreviations

AHU

antiorthostatic hindlimb unloading

ACh

acetylcholine

AChE

acetylcholinesterase

HMS

hypogravity motor syndrome

MEPPs

miniature end plate potentials

MEPCs

miniature end plate currents

EPPs

end plate potentials

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • O. V. Tyapkina
    • 1
    • 2
  • L. F. Nurullin
    • 1
    • 2
  • K. A. Petrov
    • 1
    • 3
  • E. M. Volkov
    • 2
  1. 1.Kazan Institute of Biochemistry and Biophysics, Kazan Scientific CenterRussian Academy of SciencesKazan, TatarstanRussia
  2. 2.Ministry of Health of the Russian FederationKazan State Medical UniversityKazan, TatarstanRussia
  3. 3.Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific CenterRussian Academy of SciencesKazan, TatarstanRussia

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