Cell and Tissue Biology

, Volume 8, Issue 4, pp 313–320 | Cite as

Distribution of bone-marrow stromal cells in a 3D scaffold depending on the seeding method and the scaffold inside a surface modification

  • Yu. A. Nashchekina
  • P. O. Nikonov
  • V. M. Mikhailov
  • G. P. Pinaev


The distribution of bone-marrow stromal cells (BMSC) was studied in 3D polylactide scaffolds. Seeding of cells into the scaffold by the dynamic method (with the aid of a peristaltic pump) has been shown to provide distribution of cells throughout the entire scaffold volume, unlike the static method of seeding, in which the cell suspension is applied onto the scaffold surface. Unlike the cells seeded into the scaffold by the dynamic method, the cells seeded by the static method practically completely migrate from the scaffold on the dish for the first several days. It is revealed that BMSCs cultivated in 3D polylactide scaffolds modified by fibrin form colonies, whereas BMSCs cultivated inside scaffolds modified by collagen type 1 distribute all over the scaffold volume in the form of individual cells.


3D polylactide scaffolds collagen of type 1 fibrin bone marrow stromal cells 



bone-marrow stromal cell


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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • Yu. A. Nashchekina
    • 1
    • 2
  • P. O. Nikonov
    • 1
  • V. M. Mikhailov
    • 1
  • G. P. Pinaev
    • 1
  1. 1.Institute of CytologyRussian Academy of SciencesSt. PetersburgRussia
  2. 2.Department of Chemistry of High Molecular CompoundsSt. Petersburg State UniversitySt. PetersburgRussia

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