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Ultrastructural organization and composition of carotenoids of the eyespot in Chlamydomonas reinhardtii mutants

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Abstract

Biogenesis of the ultrastructure of the eyespot in chloroplasts of unicellular green alga Chlamydomonas reinhardtii has been studied. It was established that development of the eyespot structure correlates with the accumulation of carotenoids. Due to their accumulation, the eyespot forms from one to four layers of lipid-carotenoid globules. It has been shown that, in eyespot globules, only carotenes are accumulated. It was found for the first time that, in mutants, the carotene composition in the eyespot may be changed due to changes of their composition in chloroplast membranes.

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References

  • Beckmann, M. and Hegemann, P., In vitro identification of rhodopsin in the green alga Chlamydomonas, Biochemistry, 1991, vol. 30, pp. 3692–3697.

    Article  CAS  PubMed  Google Scholar 

  • Derguini, F., Mazur, P., Nakanishi, K., Starace, D.M., Saranak, J., and Foster, K.W., All-trans-retinal is the chromophore bound to the photoreceptor of the alga Chlamydomonas reinhardtii, Photoehem. Photobiol., 1991, vol. 54, pp. 1017–1021.

    Article  CAS  Google Scholar 

  • Dieckmann, C.L., Eyespot placement and assembly in the green alga Chlamydomonas, BioEssays, 2003, vol. 25, pp. 410–416.

    Article  PubMed  Google Scholar 

  • Foster, K.W. and Smyth, R.D., Light antennas in phototactic algae, Microbiol. Rev., 1980, vol. 44, pp. 572–630.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Foster, K.W., Saranak, J., Patel, N., Zarilli, G., Okabe, M., Kline, T., and Nakanishi, K., A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas, Nature, 1984, vol. 311, pp. 756–759.

    Article  CAS  PubMed  Google Scholar 

  • Grung, M., Kreimer, G., Calenberg, M., Melkonian, M., and Liaaen-Jensen, S., Carotenoids in the eyespot apparatus of the flagellate green alga Spermatozopsis similes: aAdaptation to the retinal-based photoreceptor, Planta, 1978, vol. 193, pp. 38–43.

    Google Scholar 

  • Hager, A., and Meyer-Bertenrath, T., Die isolierung und quantitative bestimmung der carotinoide und chlorophylle von beatten, algen and isolierten chloroplasten mit hilfe dunnschichtchromatographischer methoden, Planta, 1966, vol. 69, no. 3, pp. 198–217.

    Article  CAS  PubMed  Google Scholar 

  • Harz, H. and Hegemann, P., Rhodopsin-regulated calcium currents in Chlamydomona, Nature, 1991, vol. 351, pp. 489–491.

    Article  CAS  Google Scholar 

  • Hegemann, P., Gfirtner, W., and Uhl, R., All-trans retinal constitutes the functional chromophore in Chlamydomonas rhodopsin, Biophys. J., 1991, vol. 60, pp. 1477–1489.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hegemann, P., Hegemann, U., and Foster, K.W., Reversible bleaching of Chlamydomonas reinhardtii rhodopsin in vivo, Photochem. Photobiol., 1988, vol. 48, pp. 123–128.

    Article  CAS  PubMed  Google Scholar 

  • Inwood, W., Yoshihara, C., Zalpuri, R., Kim, K.-S., and Kustu, S., The ultrastructure of a Chlamydomonas reinhardtii mutant strain lacking phytoene synthase resembles that of a colorless alga, Mol. Plant., 2008, vol. 1, pp. 925–937.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kreimer, G., Brohsonn, U., and Melkonian, M., Isolation and partial characterization of the photoreceptive organelle for phototaxis of a flagellate green alga, Eur. J. Cell Biol., 1991, vol. 55, pp. 318–327.

    CAS  PubMed  Google Scholar 

  • Kreimer, G. and Melkonian, M., Reflection confocal laser scanning microscopy of eyespots in flagellated green algae, Eur. J. Cell Biol., 1990, vol. 53, pp. 101–111.

    CAS  PubMed  Google Scholar 

  • Kreimer, G., Overlander, C., Sineschekov, O.A., Stolzis, H., Nultsch, W., and Melkonian, M., Functional analysis of the eyespot in Chlamydomonas reinhardtii mutant ey 627, mt(-), Planta, 1992, vol. 188, pp. 513–521.

    Article  CAS  PubMed  Google Scholar 

  • Ladygin, V.G. and Shirshikova, G.N., Involvement of photosystem-I in light-induced conversion of violaxanthin to zeaxanthin, Physiol. Rast., 1987, vol. 34, no. 6, pp. 1068–1072.

    CAS  Google Scholar 

  • Ladygin, V.G., Collection of the mutant Chlamydomonas strains of the Institute of Soil Science and Photosynthesis, Pushchino, in Katalog kul’tur mikrovodoroslei v kollektsiyakh SSSR (Catalogue of Microalgal Cultures in the Collections of the USSR), Moscow: Izd. Ross. Akad. Nauk, 1991, pp. 152–173.

    Google Scholar 

  • Ladygin, V.G., Pigment mutations of Chlamydomonas reinhardtii induced by N-nitrosoethylurea and by UV-irradiation, Genetika, 1970, vol. 6, no. 3, pp. 127–131.

    Google Scholar 

  • Ladygin, V.G., Semenova, G.A., and Tageeva, S.V., Plastid lamellar structure development in the Chlamydomonas reinhardi pigment mutants, Tsitologiia, 1973, vol. 15, no. 7, pp. 810–819.

    CAS  PubMed  Google Scholar 

  • Lawson, M.A. and Satir, P., Characterization of the eyespot regions of “Blind” Chlamydomonas mutants after restoration of photophobic responses, J. Eukaryotic Microbiol., 1995, vol. 41, pp. 593–601.

    Article  Google Scholar 

  • Melkonian, M. and Robenek, H., The eyespot apparatus of flagellated green algae: a critical review, Prog. Phycol. Res., 1984, vol. 3, pp. 193–268.

    Google Scholar 

  • Nultsch, W. and Hader, D.-P., Photomovement in motile microorganisms-II, Photochem. Photobiol., 1988, vol. 47, pp. 837–869.

    Article  CAS  PubMed  Google Scholar 

  • Renninger, S., Backendorf, E., and Kreimer, G., Sub fractionation of eyespot apparatuses from the green alga Spermatozopsis similis: isolation and characterization of eyespot globules, Planta, 2001, vol. 213, pp. 51–63.

    Article  CAS  PubMed  Google Scholar 

  • Riiffer, U., and Nultsch, W., Flagellar photoresponses of Chlamydomonas cells held on micropipettes. II. Change in flagellar beat pattern, Cell Motil. Cytoskeleton, 1991, vol. 18, pp. 269–278.

    Article  Google Scholar 

  • Semenova, G.A. and Ladygin, V.G., The ultrastructure of plastids of three mutant types of Chlamydomonas reinhardtii phenotypically yellow in the light or in the darkness, Tsitologiia, 1975, vol. 17, no. 9, pp. 1003–1008.

    CAS  PubMed  Google Scholar 

  • Semenova, G.A., Ultrastructural organization of Chlamydomonas eyespot, Tsitologiia, 1978, vol. 20, no. 6, pp. 603–606.

    Google Scholar 

  • Sineshchekov, O.A., Govorunova, U.G., and Litvin, F.F., Role of photosynthetic apparatus and stigma in the formation of spectral sensitivity of phototaxis in flagellated green algae, Biofizika, 1989, vol. 34, no. 2, pp. 255–258.

    CAS  Google Scholar 

  • Takahashi, T., Yoshihara, K., Watanabe, M., Kubota, M., Johnson, R., Derguini, F., and Nakanishi, K., Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: simultaneous measurements of phototactic and photophobic responses, Biochem. Biophys. Res. Commun., 1991, vol. 178, pp. 1273–1279.

    Article  CAS  PubMed  Google Scholar 

  • Wagner, V., Ulmann, K., Mollwo, A., Kaminski, M., Mittag, M., and Kreimer, G., Phosphoproteome of Chlamydomonas reinhardtii eyespot fraction includes key proteins of light signaling pathway, Plant Physiol., 2008, vol. 146, pp. 772–788.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    V. G. Ladygin

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    G. A. Semenova

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  1. V. G. Ladygin
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  2. G. A. Semenova
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Correspondence to V. G. Ladygin.

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Original Russian Text © V.G. Ladygin, G.A. Semenova, 2014, published in Tsitologiya, 2014, Vol. 56, No. 1, pp. 48–56.

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Ladygin, V.G., Semenova, G.A. Ultrastructural organization and composition of carotenoids of the eyespot in Chlamydomonas reinhardtii mutants. Cell Tiss. Biol. 8, 267–275 (2014). https://doi.org/10.1134/S1990519X14030079

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  • DOI: https://doi.org/10.1134/S1990519X14030079

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