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Interpopulation differences in hemocyte DNA-comet parameters of snail Lymnaea stagnalis from regions with different environmental loads

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Abstract

Research on hemocytes of snail Lymnaea stagnalis from regions with different environmental loads has been carried out by comet assay. Significant interpopulation differences in hemocyte DNA-comet parameters and sensitivity of hemocyte genetic material to external damaging factors (heavy-metal Sr, in particular) have been revealed by means of software analysis of hemocyte DNA-comet images. Two snail populations are characterized by high genetic identity; therefore, the different proliferative activity in the hemocytes of snail Lymnaea stagnalis from different ecological zones revealed by comet assay may be considered as an indicator of the intensity of damaging effects and environmental quality.

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References

  • Afonin, V.Yu., Osipov, A.N., Vorob’eva, N.Yu., Golubev, A.P., Koneva, A.Yu., and Smol’yanova, I.V., DNA comet assay of mantle fluid cells of snail Lymnaea stagnalis derived in laboratory conditions from animals collected from natural populations in areas with different degree of radionuclide contamination, in Dvadtsat’ let Chernobyl’skoy katastrofy. Vzglyad v buduschee (Twenty Years After the Chernobyl Disaster. A Look into the Future), Kyiv: Tsentr KhOLTEKh, 2006, pp. 251–256.

    Google Scholar 

  • Anisimov, A.P., Somatic polyploidy in individual and historical development of organisms, Tsitologiia, 1986, vol. 28, no. 10, pp. 11–25.

    Google Scholar 

  • Anisimov, A.P., Cell proliferation and somatic polyploidy in tissues of gastropods: an overview. I. Introduction. Internal tissues, Tsitologiia, 1998, vol. 40, no. 4, pp. 323–331.

    Google Scholar 

  • Anisimov, A.P., Cell proliferation and somatic polyploidy in tissues of gastropods: an overview. VII. Somatic polyploidy as a morphogenetic factor, Tsitologiia, 1999, vol. 41, no. 1, pp. 32–39.

    Google Scholar 

  • Barlow, P.W., Endopolyploidy: towards an understanding of its biological significance, Acta Biotheoret., 1978, vol. 27, pp. 1–18.

    Article  CAS  Google Scholar 

  • Belarussian document. Maximum permissible concentration (MPC) of chemicals in water of water bodies of household drinking, cultural, and residential use, in Sbornik gigienicheskikh normativov po razdelu kommunal’noy gigieny: GN 1.1.9-23-2002 i dr. (Collection of Hygiene Standards for the Communal Hygiene Section: GN 1.1.923-2002 etc.), Minsk: Minzdrav RB, 2004, pp. 37–92.

    Google Scholar 

  • Collins, A.R., The comet assay for DNA damage and repair, principles, applications, and limitations, Mol. Biotechnol., 2004, vol. 26, pp. 249–261.

    Article  PubMed  CAS  Google Scholar 

  • Golubev, A.P., Afonin, V., Maksimova, S., and Androsov, V., The current state of pond snail Lymnaea stagnalis populations from water reservoirs of the Chernobyl nuclear accident zone, Radioprotection, 2005, vol. 40, pp. S511–S517.

    Article  Google Scholar 

  • Golubev, A.P., Sikorskii, V.G., Kalinin, V.N., Afonin, V.Yu., and Chekan, G.S., The radioactive contamination dynamics of water body ecosystems of different types in the Chernobyl atomic station alienation zone in Belarus, Radiats. Biol. Radioekol., 2007, vol. 47, no. 3, pp. 322–332.

    CAS  Google Scholar 

  • Helma, C., and Uhl, M., A Public Domain Image-analysis Program for the Single-cell Gel-electrophoresis (comet) Assay, Mutation Res, 2000, vol. 466, pp. 9–15.

    Article  PubMed  CAS  Google Scholar 

  • Jaoszyn-ski, P., Kujawski, M., Czub-Swierczek, M., Markowska, J., and Szyfter, K., Bleomycin-induced DNA Damage and its Removal in Lymphocytes of Breast Cancer Patients Studied by Comet Assay, Mutat. Res., 1997, vol. 385, pp. 223–233.

    Article  Google Scholar 

  • Koneva, A.Yu., Afonin, V.Yu., and Dromashko, S.E., The age-related changes in hemolymph cellular composition and in the spectrum of cytomorphological traits of hemocyte genetic damages in snail Lymnaea stagnalis, Tsitol. Genet., 2006, vol., no. 6, pp. 21–27.

    Google Scholar 

  • Koneva, A.Yu., Genetic evaluation of Lymnaea stagnalis populations in regions with different anthropogenic load as the first step in genetic monitoring, Ekol. Genet., 2012, vol. 10, no. 2, pp. 20–30.

    Google Scholar 

  • Lizunova, E.Yu., Changes in the radiosensitivity of generations of irradiated mammalian cells, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Moscow: Inst. Khim. Fiz. Ros. Akad. Nauk, 2009.

    Google Scholar 

  • Myazin, A.E., Osipov, A.N., Elakov, A.L., Sypin, V.D., and Shevchenko, V.A., Estimation of single-strand DNA breaks in spleen lymphocytes at late term after acute irradiation of mice at sublethal and median lethal doses by comet assay, Radiats. Biol. Radioekol., 2002, vol. 42, no. 6, pp. 731–734.

    CAS  Google Scholar 

  • Olive, P.L., Bana’th, J.P., and Durand, R.E., DNA heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the “comet” assay, Radiat. Res., 1990, vol. 122, pp. 86–94.

    Article  PubMed  CAS  Google Scholar 

  • Olive, P.L., Wlodek, D., and Bana’th, J.P., DNA doublestrand breaks measured in individual cell subjected to gel electrophoresis, Cancer Res., 1991, vol. 51, pp. 4671–4676.

    PubMed  CAS  Google Scholar 

  • Ostling, O. and Johanson, K.J., Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells, Biochem. Biophys. Res. Commun., 1984, vol. 123, pp. 291–298.

    Article  PubMed  CAS  Google Scholar 

  • Rojas, E., Lopez, M.C., and Valverde, M., Single cell gel electrophoresis: methodology and applications, J. Chromatogr., 1999, vol. B 722, pp. 225–254.

    Google Scholar 

  • Rydberg, B. and Johanson, K.J., Estimation of DNA Strand Breaks in Single Mammalian Cells, New York: Academic Press, 1978, pp. 465–468.

    Google Scholar 

  • Sakharov, D.A. and Rozsa, K.S., Defensive behaviour in the pond snail Lymnaea stagnalis: the whole body withdrawal associated with exsanguinations, Acta Biol. Hung., 1989, vol. 40, pp. 329–341.

    PubMed  CAS  Google Scholar 

  • Singh, N.P., McCoy, M.T., Tice, R.R., and Schneider, E.L., A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res., 1988, vol. 175, pp. 184–191.

    Article  PubMed  CAS  Google Scholar 

  • Sirota, N.P. and Kuznetsova, E.A., The comet assay application in radiobiological investigations, Radiats. Biol. Radioekol., 2010, vol. 50, no. 3, pp. 329–339.

    CAS  Google Scholar 

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  1. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk, Belarus

    A. Yu. Koneva

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  1. A. Yu. Koneva
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Correspondence to A. Yu. Koneva.

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Original Russian Text © A.Yu. Koneva, 2013, published in Tsitologiya, 2013, Vol. 55, No. 7, pp. 475–481.

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Koneva, A.Y. Interpopulation differences in hemocyte DNA-comet parameters of snail Lymnaea stagnalis from regions with different environmental loads. Cell Tiss. Biol. 7, 472–478 (2013). https://doi.org/10.1134/S1990519X13050052

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  • DOI: https://doi.org/10.1134/S1990519X13050052

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