Cell and Tissue Biology

, Volume 7, Issue 3, pp 207–211 | Cite as

Optimal protocols of hematopoietic stem cell expansion in vitro



Hematopoietic stem cells (HSCs) effectively and continuously replenish the full range of blood-cell populations. Bone-marrow and umbilical-cord blood stem-cell transplantation (SCT) restore hematopoiesis when used in various hematological and oncohematological disorders in adults and children. However, wider clinical application of effective SCT-based approaches is limited by the low number of primitive HSCs in the available biospecimens. Development of effective protocols of HSC expansion in vitro is therefore necessary. In this review, the notion of bone marrow hematopoiesis is discussed as a complex cellular system and a comparative analysis of various methods for HSC expansion in vitro is provided. The review is illustrated by our own data supporting application of various feeder-cell types for human HSC expansion in vitro.


hematopoietic stem cells mesenchymal stem cells 



hematopoietic stem cells


mesenchymal stem cells


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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  1. 1.Almazov Federal Center for Heart, Blood, and EndocrinologySt. PetersburgRussia
  2. 2.Institute of CytologyRussian Academy of SciencesSt. PetersburgRussia

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