Skip to main content
SpringerLink
Log in

Characteristics of capsaicin-sensitive afferent neurons of spinal nerve ganglia

  • Published:
Cell and Tissue Biology Aims and scope Submit manuscript

Abstract

Morphological peculiarities of neurons that had TRPV1, SP, CGRP, and NF200 markers in thoracic spinal ganglia nerves were studied in 3-month-old rats subjected to chemical deafferentation produced by capsaicin. The obtained results have shown that from 6.5 to 41.3% of ganglion neurons in the control group of animals contain, as a rule, one of the above-mentioned markers. The heterogeneity of nociceptive neurons observed in a control group of rats was also preserved in the capsaicin-treated animals. In both groups, the spinal ganglion TRPV1 neurons were predominant, whereas populations of SP, CGRP, and NF200 neurons formed smaller groups. In each population, sensitivity to capsaicin was shown in the largest neurons, regardless of marker; this sensitivity was pronounced to the greatest degree in the group of TRPV1 neurons.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

SGTN:

sensitive of the second thoracic spinal nerve

CGRP:

calcitonin gene-related peptide

NF200:

protein of neurofilaments with mol. mass of 200 kDa

SP:

substance P

TRPV1:

TRPV1-receptors (transient receptor potential vanilloid receptor 1)

References

  • Akopian, A.N., Sivilotti, L., and Wood, J.N., A Tetrodotoxin-Resistant Voltage-Gated Sodium Channel Expressed by Sensory Neurons, Nature, 1996, vol. 379, pp. 257–262.

    Article  PubMed  CAS  Google Scholar 

  • Bersenev, V.A., Sheinye spinnomozgovye uzly (Cervical Spinal Ganglia), Moscow: Meditsina, 1980.

    Google Scholar 

  • Brzozowski, T., Konturek, S.J., Sliwowski, Z., Pytko-Polon’czyk, J., Szlachcic, A., and Drozdowicz, D., Role of Capsaicin-Sensitive Sensory Nerves in Gastroprotection against Acid-Independent and Acid-Dependent Ulcerogens, Digestion, 1996, vol. 57, pp. 424–432.

    Article  PubMed  CAS  Google Scholar 

  • Bucelli, R.C., Gonsiorek, E.A., Kim, W.-Y., Bruun, D., Rabin, R.A., Higgins, D., and Lein, P.J., Statins Decrease Expression of the Proinflammatory Neuropeptides Calcitonin Gene-Related Peptide and Substance P in Sensory Neurons, Pharmacol. Exp. Ther., 2008, vol. 324, pp. 1172–1180.

    Article  CAS  Google Scholar 

  • Cao, X., Cao, X., Xie, H., Yang, R., Lei, G., Li, F., Li, A., Liu, C., and Liu, L., Effects of Capsaicin on VGSCs in TRPV1-/- Mice, Brain Res., 2007, vol. 1163, pp. 33–43.

    Article  PubMed  CAS  Google Scholar 

  • Cardenas, C.G., Del, Mar, L.P., Cooper, B.Y., and Scroggs, R.S., 5HT4 Receptors Couple Positively to Tetrodotoxin-Insensitive Sodium Channels in a Subpopulation of Capsaicin-Sensitive Rat Sensory Neurons, Neuroscience, 1997, vol. 17, pp. 7181–7189.

    PubMed  CAS  Google Scholar 

  • Gold, M.S., Reichling, D.B., Shuster, M.J., and Levine, J.D., Hyperalgesic Agents Increase a Tetrodotoxin-Resistant Na Current in Nociceptors, Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 1108–1112.

    Article  PubMed  CAS  Google Scholar 

  • Holzer, P., Peptidergic Sensory Neurons on the Control of Vascular Functions Mechanisms and Significance of the Cutaneous and Splanchnic Vascular Beds, Rev. Physiol. Biochem. Pharmacol., 1992, vol. 121, pp. 49–146.

    Article  PubMed  CAS  Google Scholar 

  • Lai, J., Porreca, F., Hunter, J.C., and Gold, M.S., Voltage-Gated Sodium Channels and Hyperalgesia, Ann. Rev. Pharmacol. Toxicol., 2004, vol. 44, pp. 371–397.

    Article  CAS  Google Scholar 

  • Ma, Q.P., Expression of Capsaicin Receptor (VR1) by Myelinated Primary Afferent Neurons in Rats, Neurosci. Lett., 2002, vol. 319, pp. 87–90.

    Article  PubMed  CAS  Google Scholar 

  • Masliukov, P.M. and Timmermans, J.-P., Immunocytochemical Properties of Stellate Ganglion Neurons during Early Postnatal Development, Histochem. Cell Biol., 2004, vol. 122, pp. 201–209.

    Article  PubMed  CAS  Google Scholar 

  • Maslyukov, P.M., Shilkin, V.V., Emanuylov, A.I., and Korzina, M.B., Neurotransmitter Composition of Neurons of the Cranial, Cervical, and Splanchnic Sympathetic Ganglia in Postnatal Ontogenesis, Morfologiya, 2009, vol. 135, no. 1, pp. 30–34.

    Google Scholar 

  • McLain, R.F. and Weinstein, J.N., orphometric Model of Normal Rabbit Dorsal Root Ganglia, Spine, 1993, vol. 1, pp. 1746–1752.

    Article  Google Scholar 

  • Piper, A.S. and Docherty, R.J., One-Way Cross-Desensitization between P2X Purinoceptors and Vanilloid Receptors in Adult Rat Dorsal Root Ganglion Neurons, Physiology, 2000, vol. 15, pp. 685–696.

    Google Scholar 

  • Porseva, V.V., Segmental Differences in the Postnatal Development of Afferent Neurocytes of the White Rat, Morfol. Vedom., 2009, vol. 135, nos. 1–2, pp. 46–48.

    Google Scholar 

  • Porseva, V.V., Shilkin, V.V., Korzina, M.B., Korobkin, A.A., and Maslyukov, P.M., Changes in the TRPV1-Immunoreactive Neurons in the Sensitive Ganglia of Spinal Nerves of the Rat under the Influence of Capsaicin, Morfologiya, 2011, vol. 139, no. 3, pp. 41–45.

    CAS  Google Scholar 

  • Price, T.J. and Flores, C.M., Critical Evaluation of the Colocalization between Calcitonin Gene-Related Peptide, Substance P, Transient Receptor Potential Vanilloid Subfamily Type 1 Immunoreactivities, and Isolectin B4 Binding in Primary Afferent Neurons of the Rat and Mouse, Pain, 2007, vol. 8, pp. 263–272.

    CAS  Google Scholar 

  • Revenko, S.V., Borovikova, L.V., Borovikov, D.V., and Ermishkin, V.V., Resistance of the Terminal Polymodal C-Units of the Cat Skin to during Mechanical and Heat Stimulation, Byull. Eksp. Biol. Med., 1997, vol. 124, no. 10, pp. 369–371.

    CAS  Google Scholar 

  • Rumyantseva, T.A., Changes in the Neurocytes of Intramural Gastric Ganglia in Desympathetized and Deafferented White Rat, Morfologiya, 2001, vol. 120, no. 4, pp. 82–83.

    Google Scholar 

  • Somjen, G.G., Sensory Coding in the Mammalian Nervous System, New York: Plenum Pub., 1975.

    Book  Google Scholar 

  • Suzuki, M., Watanabe, Y., Oyama, Y., Mizuno, A., Kusano, E., and Hirao, A., Localization of Mechanosensitive Channel TRPV4 in Mouse Skin, Neurosci. Lett., 2003, vol. 353, pp. 189–192.

    Article  PubMed  CAS  Google Scholar 

  • Yasuchika, A., Seiji, O., Kazuhisa, T., Hidetoshi, I., Hideo, D., Tomoyuki, O., Tomoko, S., and Hideshige, M., Expression and Co-Expression of VR1, CGRP, and IB4-Binding Glycoprotein in Dorsal Root Ganglion Neurons in Rats: Differences between the Disc Afferents and the Cutaneous Afferents, Spine, 2005, vol. 30, pp. 1496–1500.

    Article  Google Scholar 

  • Yoshimura, N., Erdman, S.L., Snider, M.W., and de Groat, W.C., Effects of Spinal Cord Injury on Neurofilament Immunoreactivity and Capsaicin Sensitivity in Rat Dorsal Root Ganglion Neurons Innervating the Urinary Bladder, Neuroscience, 1998, vol. 83, pp. 633–643.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, State Medical Academy of Ministry of Health Service and Social Development, Yaroslavl, Russia

    V. V. Porseva

Authors
  1. V. V. Porseva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. V. Porseva.

Additional information

Original Russian Text © V.V. Porseva, 2012, published in Tsitologiya, 2012, Vol. 54, No. 12, pp. 887–891.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Porseva, V.V. Characteristics of capsaicin-sensitive afferent neurons of spinal nerve ganglia. Cell Tiss. Biol. 7, 149–153 (2013). https://doi.org/10.1134/S1990519X13020119

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990519X13020119

Keywords

Search

Navigation