Cell and Tissue Biology

, Volume 6, Issue 5–6, pp 485–489 | Cite as

Proliferation and cell death of hepatocytes in regenerating fetal rat liver

  • A. V. Elchaninov
  • G. B. Bolshakova


Proliferation and death of hepatocytes in regenerating liver were studied in 17-day-old fetal white rats. Two days after liver resection (20%), animals were sacrificed every 3 h. In experimental groups, the index of Ki67-positive hepatocytes increased sharply 15 h after liver resection. In all experimental and control groups, the ratio of the index of the metaphase, the longest phase of mitosis, to the mitotic index remained unchanged, indicating the same duration of hepatocyte mitoses in regenerating liver. In regenerating and intact liver, hepatocytes labeled with antibodies to caspase 3 were not detected. Thus, resection of 20% fetal rat liver did not promote enhancement of apoptosis of hepatocytes.


regeneration liver embryogenesis hepatocytes proliferation apoptosis 



index of Ki67


mitotic index


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© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  1. 1.Research Institute of Human MorphologyRussian Academy of Medical SciencesMoscowRussia

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