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Cell and Tissue Biology

, 5:388 | Cite as

Effect of magnetic nanoparticles Fe3O4 on viability, attachment, and spreading of isolated fetuses and newborn rats

  • A. N. Sukach
  • A. S. Lebedinskii
  • V. I. Grishchenko
  • T. D. Lyashenko
Article

Abstract

This study has shown that the toxic effect of nonmodified Fe3O4 nanoparticles in vitro depends on the metabolic and morphological conditions of cells from rat fetuses and newborns. In the process of cultivation, cells with magnetic nanoparticles bind to their surface and penetrate the intracellular space. The sorption of nanoparticles on the cell surface hinders their attachment to the substrate and absorption by spread cells can prevent their proliferation. Magnetic nanoparticles are well sorbed by the upper layer of cell aggregates, whereas cells of the inner layer remain intact. As a result, the cell aggregates acquire the property of responding to a constant magnetic field. These aggregates could potentially be used in cell transplantation for directed cell delivery.

Keywords

isolated cells cultivation magnetic nanoparticles lipid peroxidation toxicity 

Abbreviations used

NRLC

newborn rat liver cell

RFLC

rat fetal liver cell

MDA

malone dialdehyde

NRNC

newborn rat neural cell

RFNC

rat fetal neural cell

FNP

ferromagnetic Fe3O4 nanoparticle

TBA

thiobarbituric acid

FF

rat fetal fibroblast

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • A. N. Sukach
    • 1
    • 2
  • A. S. Lebedinskii
    • 1
  • V. I. Grishchenko
    • 1
  • T. D. Lyashenko
    • 2
  1. 1.Institute for Problems of Cryobiology and CryomedicineNational Academy of Sciences of UkraineKharkovUkraine
  2. 2.Skovoroda National Pedagogical UniversityKharkovUkraine

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