Cell and Tissue Biology

, Volume 5, Issue 1, pp 62–67 | Cite as

A subset of cells in tobacco mosaic virus (TMV)-induced local lesions in Datura stramonium leaves are tolerant to TMV

  • A. V. Reunov
  • S. N. Lega
  • V. P. Nagorskaya
  • L. A. Lapshina


Ultrastructural examination of tobacco mosaic virus-induced local lesions growing in Datura stramonium leaves is carried out. It is demonstrated that, in the central area of the lesions, the cell response to viral invasion is not uniform. Most cells exhibited an acute hypersensitive reaction (HR) and underwent rapid and complete necrosis. However, some cells, despite considerable virus accumulation and immediate contact with completely collapsed cells, maintained a certain degree of structural integrity. Analysis performed showed that the proportion of collapsed and uncollapsed cells in the lesion centre 3 to 5 days after infection essentially did not change. These data suggest that the absence of HR in some cells in the lesion centre is not due to an early stage of infection but is likely caused by cell tolerance of the virus.


Cell ultrastructure Datura stramonium leaves hypersensitive reaction tobacco mosaic virus 



endoplasmic reticulum


hypersensitive reaction


programmed cell death


tobacco mosaic virus


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  1. Belitser, N.V., Lysosomal System and Microbodies in Plant and Animal Cells, Extended Abstract of Doctoral (Biol.) Dissertation, Leningrad, 1978.Google Scholar
  2. Chichkova, N.V., Kim, S.H., Titova, E.S., Kalkum, M., Morozov, V.S., Rubtsov, Y.P., Kalinina, N.O., Taliansky, M.E., and Vartapetian, A.B., A Plant Caspase-Like Protease Activated During the Hypersensitive Response, Plant Cell, 2004, vol. 16, pp. 157–171.PubMedCrossRefGoogle Scholar
  3. Del Duca, S., Betti, L., Trebbi, G., Serafini-Fracassini, D., and Torrigiani, P., Transglutaminase Activity Changes During the Hypersensitive Reaction, a Typical Defense Response of Tobacco NN Plants to TMV, Physiol. Plant., 2007, vol. 131, pp. 241–250.PubMedGoogle Scholar
  4. Esau, K., Viruses in Plant Hosts. Form, Distribution and Pathologic Effects, Madison: Univ. Wisconsin Press, 1968.Google Scholar
  5. Favali, M.A., Bassi, M., and Conti, G.G., Morphological, Cytochemical and Autoradiographic Studies of Local Lesions Induced by the U5 Strain of Tobacco Mosaic Virus in Nicotiana glutinosa L., Rivista di Patologia Vegetale. Series IV, 1974, vol. 10, pp. 207–218.Google Scholar
  6. Gechev, T.S. and Hille, J., Hydrogen Peroxide As a Signal Controlling Plant Programmed Cell Death, J. Cell Biol., 2005, vol. 168, pp. 17–20.PubMedCrossRefGoogle Scholar
  7. Hammond-Kosack, K.E. and Jones, J.D.G., Resistance Gene-Dependent Plant Defense Responses, Plant Cell, 1996, vol. 8, pp. 1773–1791.PubMedCrossRefGoogle Scholar
  8. Hatsugai, N., Kuroyanagi, M., Yamada, K., Meshi, T., Tsuda, S., Kondo, M., Nishimura, M., and Hara-Nishimura, I., A Plant Vacuolar Protease, VPE, Mediated Virus-Induced Hypersensitive Cell Death, Science, 2004, vol. 305, pp. 855–858.PubMedCrossRefGoogle Scholar
  9. Hayashi, T. and Matsui, C., Fine Structure of Lesion Periphery Produced by Tobacco Mosaic Virus, Phytopathology, 1965, vol. 55, pp. 387–392.PubMedGoogle Scholar
  10. Heath, M.C., Apoptosis, Programmed Cell Death and the Hypersensitive Response, Eur. J. Plant Pathol., 1998, vol. 104, pp. 117–124.CrossRefGoogle Scholar
  11. Israel. H.W. and Ross, A.F., The Fine Structure of Local Lesions Induced by Tobacco Mosaic Virus in Tobacco, Virology, 1967, vol. 33, pp. 272–286.PubMedCrossRefGoogle Scholar
  12. Lam, E. and del Pozo, O., Caspase-Like Proteases Involvement in the Control of Plant Cell Death, Plant Mol. Biol., 2000, vol. 44, pp. 417–428.PubMedCrossRefGoogle Scholar
  13. Lamb, C.J., Plant Disease Resistance Genes in Signal Perception and Transduction, Cell, 1994, vol. 76, pp. 419–422.PubMedCrossRefGoogle Scholar
  14. Loebenstein, G., Localization and Induced Resistance in Virus-Infected Plants, Annu. Rev. Phytopathol., 1972, vol. 10, pp. 177–206.CrossRefGoogle Scholar
  15. Marathe, R., Anandalakshmi, R., Liu, Y., and Dinesh-Kumar, S.P., The Tobacco Mosaic Virus Resistance Gene, N. Mol. Plant Pathol., 2002, vol. 3, pp. 167–172.CrossRefGoogle Scholar
  16. Milne, R.G., Electron Microscopy of Tobacco Mosaic Virus in Leaves of Nicotiana glutinosa, Virology, 1966, vol. 28, pp. 527–532.PubMedCrossRefGoogle Scholar
  17. Mittler, R. and Lam, E., Identification, Characterization, and Purification of a Tobacco Endonuclease Activity Induced upon Hypersensitive Response Cell Death, Plant Cell, 1995, vol. 7, pp. 1951–1962.PubMedCrossRefGoogle Scholar
  18. Park, J.M., The Hypersensitive Response. A Cell Death During Disease Resistance, Plant Pathol. J., 2005, vol. 21, pp. 99–101.CrossRefGoogle Scholar
  19. Reunov, A.V., Virusnyi patogenez i zashchitnye mekhanizmy rastenii (Viral Pathogenesis and Defensive Mechanisms of Plants), Vladivostok: Dal’nauka, 1999.Google Scholar
  20. Reunov, A.V., Cytology of Plant Cell Damaged by Viruses (TMV, PVX) and the Problem of Plant Resistance, Extended Abstract of Doctoral (Biol.) Dissertation, Kiev, 1989.Google Scholar
  21. Reunov, A.V., Reunova, G.D., Nagorskaya, V.P., Lapshina, L.A., and Lega, S.N., Destruction of Tobacco Mosaic Virus in Developing Local Lesions in Leaves of Datura stramonium L., Acta Phytopathol. Entomol. Hung., 1996, vol. 31, nos. 1–2, pp. 11–17.Google Scholar
  22. Robinson, D.G., Clathrin-Mediated Trafficking, Trends Plant Sci., 1996, vol. 1, pp. 349–355.Google Scholar
  23. Yoda, H., Yamaguchi, Y., and Sano, H., Induction of Hypersensitive Cell Death by Hydrogen Peroxide Produced Through Polyamine Degradation in Tobacco Plants, Plant Physiol., 2003, vol. 132, pp. 1973–1981.PubMedCrossRefGoogle Scholar
  24. Zavarzin, A.A. and Kharazova, A.D., Osnovy obshchei tsitologii (Fundamentals of General Cytology), Leningrad: Leningr. Gos. Univ., 1982.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • A. V. Reunov
    • 1
  • S. N. Lega
    • 1
  • V. P. Nagorskaya
    • 1
  • L. A. Lapshina
    • 1
  1. 1.Pacific Institute of Bioorganic ChemistryFar-East Branch of Russian Academy of SciencesVladivostokRussia

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