Dynamics of calcium ions accumulation and changes of isoforms of sarcoplasmic reticulum Ca-ATPase (SERCA) in soleus muscle fibers of rats and Mongolian gerbils under gravitational unloading of various duration
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The concentration of calcium ions in soleus muscle fibers in Mongolian gerbils and rats has been shown to increase for the first day of gravitational load by 4.5 and 2.8 times, respectively, compared to the control. This increase is preserved for 3 days, while, after 12 days, it decreases, but remains above the control level. The resting calcium level in the control in gerbils is lower than in rats and, as a consequence, its change for the first day of unloading is greater. It is possible that this leads to earlier changes in the muscle fiber isoform composition in gerbils; a statistically significant shift in the ratio of fibers that contain fast and slow calcium pumps towards pumping faster is noted as early as the first day, while that of fibers that express the corresponding isoforms of myosin heavy chains (MHCs) is observed after 3 days of functional unloading. In rats, similar changes are revealed as late as on the 7th day. After 12 days, in both species, a tendency to return to the control level is observed that is more pronounced in gerbil.
Keywordsmuscle fiber gravitational unloading resting calcium level SERCA
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