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Cell and Tissue Biology

, Volume 4, Issue 3, pp 228–233 | Cite as

Comparative localization of chicken five functional genes and three microsatellites on quail mitotic chromosomes by two-color FISH hybridization

  • A. V. Trukhina
  • A. F. Smirnov
Article
  • 46 Downloads

Abstract

To localize chicken genes and microsatellites, we used heterologous two-color FISH and chicken chromosome specific BAC clones. All BAC clones were verified by PCR. An analysis of the results has shown that maf gene forms one linkage group with the mc1r gene (CJA11), aldh1a1 forms one linkage group with the igvps gene (CJA15), pno forms one linkage group with the acaca gene (CJA19), fzf forms one linkage group with the bmp7 gene (CJA20), and cw01 forms one linkage group with the ubap2w gene (CJAW). Microsatellite ADL0254 was localized jointly with the insr gene (CJA28), and LEI0342 and MCW0330 microsatellites were localized jointly with the hspa5 gene (CJA17). If we consider that the nomenclature of quail chromosomes is the same as in chickens, their localization will correspond to the following chromosomes: CJA11 (maf), 15 (aldh1a1), 19 (pno), 20 (fzf), and W (cw01). The microsatellite ADL0254 turned out to be located on the same microchromosome as the insr gene (CJA 28), while microsatellites LEI0342 and MCW0330 were found to be in the same linkage group with the hspa5 gene (CJA17). The same work was also carried out on the chicken genome. Different results were obtained. The localization of the BAC clones containing the cw01 and fzf genes and the MCW0330 microsatellite was confirmed completely; they are located on GGAW, 20, and 17 chromosomes, respectively. Microsatellites ADL0254 and LEI0342 were each revealed to have two sites, whereas the localization of the remaining genes (maf, aldh1a1, and pno) on the GGA11, GGA15, and GGA19 chromosomes turned out to be untrue and needs further study.

Key words

gene linkage group microsatellite bacterial artificial chromosome (BAC) Coturnix c. japonica FISH Gallus g. domesticus 

Abbreviation used

acaca

gene of acetyl-coenzyme A carboxylase α

aldh1a1

gene of cytosol alcohol dehydrogenase A1

bmp7

gene of bone morphogenic protein 7

CJA

the quail chromosome

cw01

sequence of site of nonrepeated DNA of the chicken chromosome W

fzf

gene of Wilms’ tumor

GGA

chicken chromosome

HSA

human chromosome

hspa5

gene of 70 kDa heat-shock protein

igvps

gene of immunoglobulin, pseudo-V26 and V6

insr

gene of insulin receptor

maf

protooncogen of the chicken c-maf

mc1r

gene of melanocortin-1 receptor

MMU

mouse chromosome

pno

gene of opsin

ubap2w

ubiquitin-associated protein 2 isoform 1

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References

  1. Delany, M.E., Gessaro, T.M., Rodrigue, K.L., and Daniels, L.M., Chromosomal Mapping of Chichen Mega-Telomere Arrays to GGA9, 16, 28 and W using a Cytogenomic Approach, Cytogenet. Genome Res., 2007, vol. 117, pp. 54–63.CrossRefPubMedGoogle Scholar
  2. Kayang, B.B., Fillon, V., Inoue-Murayama, M., Miwa, M., Leroux, S., Fevel, K., Monvoisin, J.L., Pitel, F., Vignoles, M., Mouilhayrat, C., Beaumont, C., Ito, S., Minvielle, F., and Vignal, A., Integrated Maps in Quail (Coturnix japonica) Confirm the High Degree of Synteny Conservation with Chicken (Gallus gallus) Despite 35 Million Years of Divergence, BMC Genomics, 2006, vol. 7, pp. 101.CrossRefPubMedGoogle Scholar
  3. Kuznetsova, N.N. and Vinter, V.G., Metody Gennoy Inzhenerii: Uchebnoe Posobie (Genetic Engineering Methods: A Manual), Moscow: Bioinformservis, 1997.Google Scholar
  4. Lichter, P., Boyle, A.L., Cremer, T., and Ward, D.C., Analysis of Genes and Chromosomes by Nonisotopic in Situ Hybridization, Genet. Anal. Tech. App., 1991, vol. 8, pp. 24–33.Google Scholar
  5. Nanda, I., Shan, Z., Schartl, M., Burt, D.W., Koehler, M., Nothwang, H-G., Grutzner, F., Paton, I.R., Windsor, D., Dunn, I., Engel, W., Staeheli, P., Mizuno, S., Haaf, T., and Schmid, M., 300 Million Years of Conserved Synteny Between Chicken Z and Human Chromosome 9, Nature Genetics, 1999, vol. 21, pp. 258–259.CrossRefPubMedGoogle Scholar
  6. Romanov, M.N., Price, J.A., and Dodgson, J.B., 2003. Integration of Animal Linkage and BAC Contig Maps Using OVERGO Hybridization, Cytogenet. Genome Res., 1999, vol. 102, pp. 277–281.CrossRefGoogle Scholar
  7. Rozen, S. and Skaletsky, H., Primer3 on the www for General Users and for Biologist Programmers, Methods Mol. Biol., 2000, vol. 132, pp. 365–368.PubMedGoogle Scholar
  8. Schmid, M., Nanda, I., and Burt, D., First Report on Chicken Genes and Chromosomes, Cytogenet. Cell Genet., 2000, vol. 90, pp. 169–218.CrossRefPubMedGoogle Scholar
  9. Schmid, M., Nanda, I., Burt, D., Second Report on Chicken Genes and Chromosomes, Cytogenet. Genome Res., 2005, vol. 109, pp. 415–479.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Department of Genetics and SelectionSt. Petersburg State UniversitySt. PetersburgRussia

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