Interchromatin granule clusters of mouse preovulatory oocytes: Organization, molecular composition, and possible functions
- 59 Downloads
The nuclei of mouse oocytes at the late diplotene stage of meiotic prophase I contain numerous interchromatin granule clusters (IGCs), which are universal, evolutionarily conserved nuclear compartments that are specifically enriched in factors of pre-mRNA splicing. In keeping with the current concepts, IGCs may play an important role in the processes of gene expression by the coupling interconnection of its individual stages, ranging from the transcription to export of mRNA. We have obtained data on the distribution of poly(A)+ RNA, heterogenous nuclear ribonucleoprotein (hnRNP) proteins of the A/B family, and the NXF1/TAP factor of mRNA export in association with IGCs of mouse preovulatory oocytes. In the presented work, using the inhibitor of phosphatases okadaic acid, we have managed to demonstrate the dynamics of large IGCs under the conditions of specific phosphorylation of SR proteins for the first time. The studies were carried out using microinjections of oligonucleotide probes into oocytes, as well as confocal laser scanning and electron microscopy. The problem of the functional significance of IGCs in processes of pre-mRNA metabolism, as well as the participation of IGCs in the organization of mRNA export from the nucleus into the cytoplasm are discussed.
Key wordsmouse oocyte nucleus nuclear compartments interchromatin granule clusters metabolism of pre-mRNA poly(A)+ RNA export of mRNA okadaic acid electron microscopy confocal microscopy
heterogenous nuclear ribonucleoprotein
interchromatin granule cluster
Unable to display preview. Download preview PDF.
- He, Y. and Smith, R., Nuclear Functions of Heterogeneous Nuclear Ribonucleoproteins A/B, Cell Mol. Biol. Sci., 2009, vol. 66, pp. 1239–1256.Google Scholar
- Hu, Q., Kwon, Y.-S., Nunez, E., Cardamone, M.D., Hutt, K.R., Ohgi, K.A., Carcia-Bassets, I., Rose, D.W., Glass, C.K., Rosenfeld, M.G., and Fu, X.-D., Enhancing Nuclear Receptor-Induced Transcription Requires Nuclear Motor and Lsd1-Dependent Gene Networking in Interchromatin Granules, Proc. Natl. Acad. Sci. USA, 2008, vol. 105, pp. 19199–19204.CrossRefPubMedGoogle Scholar
- Izaurralde, E., Nuclear Export of Messenger RNA, Results Probe Cell Differ., 2002, vol. 35, pp. 133–150.Google Scholar
- Parfenov, V.N., Davis, D.S., Pochukalina, G.N., Kostyuchek, D., and Murti, K.G., Nuclear Distribution of RNA Polymerase II in Human Oocytes from Antral Follicles: Dynamics Relative to the Transcriptional State and Association with Splicing Factors, J. Cell. Biochem., 2000, vol. 77, pp. 654–665.CrossRefPubMedGoogle Scholar
- Parfenov, V.N., Pochukalina, G.N., Davis, D.S., Reinbold, R., Schöler, H.R., and Murti, K.G., Nuclear Distribution of Oct-4 Transcription Factor in Transcriptionally Active and Inactive Mouse Oocytes and Its Relation to RNA Polymerase II and Splicing Factors, J. Cell Biochem., 2003, vol. 89, pp. 720–732.CrossRefPubMedGoogle Scholar
- Pochukalina, G.N., Davis, D.S., Kostiuchek, D.F., Murti, K.G., and Parfenov, V.N. Splicing Factors in Oocyte Nuclei from Human Antral Follicles, Tsitologiia, 1998, vol. 40, no. 4, pp. 237–249.Google Scholar
- Tanuma, N., Kim, S.-E., Beullens, M., Tsubali, Y., Mitsuhashi, S., Nomura, M., Kawamura, T., Isono, K., Koseki, H., Sato, M., Bollen, M., Kikuchi, K., and Shima, H., Nuclear Inhibitor of Protein Phosphatase-1 (NIPP1) Direct Protein Phosphatase-1 (PP1) to Dephosphorylate the U2 snRNP Component, Spliceosome-Associated Protein 155 (Sap155), J. Biol. Chem., 2008, vol. 283, pp. 35 805–35 814.CrossRefGoogle Scholar