Cell and Tissue Biology

, Volume 2, Issue 5, pp 472–480 | Cite as

Interaction of signal cascades induced by cAMP and prolactin in bovine oocyte-cumulus complexes

  • I. Yu. Lebedeva
  • G. N. Singina
  • N. A. Volkova
  • A. N. Mormishev
  • A. K. Golubev
  • N. A. Zinovieva


Prolactin (PRL) is one of the pituitary hormones that participate in controlling mammalian folliculo-and oogenesis. In the present study, the combined action of PRL (50 ng/ml) and dibutyryl cAMP (dbcAMP, 1 mM) on oocyte maturation and the morphologic-functional state of the surrounding cumulus cells was investigated in vitro. It has been shown that PRL completely suppresses the inhibitory effect of dbcAMP on meiosis reinitiation and completion of the oocyte nuclear maturation. Moreover, PRL partly inhibited the dbcAMP-induced cumulus expansion, although it produced an opposite effect in the control medium. In the presence of PRL, the inhibitory effect of dbcAMP on the proliferative activity of cumulus cells, as well as on the PRL-induced suppression of destructive processes in these cells, was revealed. In cumulus cells, the mRNA expression of the long PRL receptor isoform was established by the RT-PCR method. The obtained data indicate an interaction of signal cascades induced by PRL and cAMP in the bovine oocyte-cumulus complexes, with the coupling site of these cascades in oocytes seeming to differ from that in cumulus cells.

Key words

prolactin cAMP oocytes cumulus cells in vitro maturation meiosis 



dibutyryl derivative of cAMP




metaphase II


oocyte-cumulus complexes


reverse transcription coupled with polymerase chain reaction




telophase I


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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • I. Yu. Lebedeva
    • 1
    • 2
  • G. N. Singina
    • 1
  • N. A. Volkova
    • 1
  • A. N. Mormishev
    • 1
  • A. K. Golubev
    • 2
  • N. A. Zinovieva
    • 1
  1. 1.All-Russian State Research Institute of Animal BreedingPodolsk, Moscow oblastRussia
  2. 2.Department of Animal Genetics, Breeding, and BiotechnologySt. Petersburg State Agrarian UniversitySt. Petersburg, PushkinRussia

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