Inhibition of phagocytic function of macrophages in vitro by dimer RNase of Bacillus intermedius
The interactions between rat peritoneal macrophage and Bacillus intermedius dimer RNase cross linked by dimethylsuberimidate was investigated in vitro. It has been found that dimer in the form of RNase at concentrations of 0.5–40μg/ml decreases the phagocytic function of macrophages. This is manifested as an inhibition of phagocytosis and suppression of the fusion of phagosomes with lysosomes in macrophages. Using atomic force microscopy, it is shown that the dimer RNase changes the surface structure of the cytoplasmic membrane more strongly than the monomer. The association between modifications of properties of the membrane and inhibition of the macrophage phagocytic function is discussed.
KeywordsRNase dimer peritoneal macrophage Bacillus intermedius RNase cytoplasmic membrane phagocytic function
atomic force microscopy
- RNase Bi
RNase of Bacillus intermedius
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- Antonov, V.F., Smirnova, E.Yu., and Shevchenko, E.V., Lipidnye membrany pri fazovykh prevrashcheniyakh (Lipid Membranes during Phase Transformations), Moscow: Nauka, 1992, 136 pp.Google Scholar
- Il’inskaya, O.N., and Makarov, A.A., Why Ribonucleases Cause Death of Cancer Cells, Molek. Biol., 2005, vol. 39, no.1, pp. 3–13.Google Scholar
- Kalacheva, N.V., and Kurinenko, B.M., Effect of Ribonucleases and Their Modified Derivatives on Functional Activity of Rat Peritoneal Macrophages, Biomedits. Khimiya, 2005, vol. 51, no. 3, pp. 303–310.Google Scholar
- Kalacheva, N.V., Narulina, A.V., and Kurinenko, B.M., Peculiarities of Electrostatic Interaction of Pancreatic and Microbial Ribonucleases with Macrophages, Tsitologiya, 2007, vol. 49, no. 4, pp. 296–299.Google Scholar
- Korkina, L.G., Suslova, T.B., Gulyaeva, Zh.G., Zezin, A.B., Velichkovskii, B.T., Vladimirov, Yu.A., and Kabanov, V.A., Membrane Mechanism of Cell Activation, Biol. Membrany, 1987, vol. 4, no. 10, pp. 1093–1101.Google Scholar
- Korkina, L.G., Suslova, T.B., Gulyaeva, Zh.G., Zezin, A.B., Velichkovskii, B.T., and Kabanov, V.A., Chemiluminescence of Peritoneal Macrophages, Activated by Non-natural Polyelectrolytes, DAN SSSR, 1985, vol. 282, no. 1, pp. 206–209.Google Scholar
- Leshchinskaya, I.B., Balaban, N.P., Kapranova, M.N., and Golubenko, I.A., Methods of Determination of Nucleases and Related Enzymes, Sovremennye metody izucheniya nukleinovykh kislot i nukleaz mikroorganizmov (Current Methods of Study of Nucleic Acids and Nucleases of Microorganisms), Kazan, Izd-vo KGU, 1980, pp. 53–60.Google Scholar
- Mayanskii, A.N., and Mayanskii, D.N., Ocherki o neuitrofile i makrofage (Essays of Neutrophil and Macrophage), Novosibirsk: Nauka, 1983, 275 pp.Google Scholar
- Plate, N.F., and Vasil’ev, A.E., Fiziologicheski aktivnye polimery (Physiologically Active Polymers), Moscow, Khimiya, 1986, 382 pp.Google Scholar