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Cell and Tissue Biology

, Volume 2, Issue 4, pp 347–353 | Cite as

Study of the pre-implantation development of mice embryos marked with a green fluorescent protein (EGFP) gene

  • A. V. Sorokin
  • E. M. Nonyashvili
  • O. V. Kidgotko
  • L. K. Sasina
  • T. D. Aleinikova
  • M. M. Shavlovskii
  • S. I. Gorodetskii
  • A. P. Dyban
Article
  • 44 Downloads

Abstract

We present the data on the in vitro development of mouse embryos after injecting pronuclei with cloned DNA fragments with an enhanced green fluorescent protein (EGFP) gene under the control of different regulatory elements (promoters, enhancers, stop signals, etc). It was found that the microinjection procedure itself inhibits development independent of the genetic constructs applied. The development of transgenic embryos may be blocked at different stages of cleavage and morula or blastocyte stages. Most transgenic embryos proved to be mosaics. Transgenic cells are very frequently found in trophectoderm; i.e. they are not a part of the inner cell mass, which is a progenitor for the development of embryo tissues.

Keywords

mouse embryos EGFP transgenic embryos activation of zygotic genes 

Abbreviations

SDS

sodium dodecyl sulfate

bp

base pairs

EDTA

sodium tetraacetic acid

EGFP

enhanced green fluorescence protein

R26

Rosa26

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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • A. V. Sorokin
    • 1
  • E. M. Nonyashvili
    • 1
  • O. V. Kidgotko
    • 1
  • L. K. Sasina
    • 1
  • T. D. Aleinikova
    • 1
  • M. M. Shavlovskii
    • 1
  • S. I. Gorodetskii
    • 2
  • A. P. Dyban
    • 1
  1. 1.Institute of Experimental MedicineRussian Academy of Medical SciencesSt. PetersburgRussia
  2. 2.Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia

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