Cell and Tissue Biology

, Volume 2, Issue 4, pp 347–353 | Cite as

Study of the pre-implantation development of mice embryos marked with a green fluorescent protein (EGFP) gene

  • A. V. Sorokin
  • E. M. Nonyashvili
  • O. V. Kidgotko
  • L. K. Sasina
  • T. D. Aleinikova
  • M. M. Shavlovskii
  • S. I. Gorodetskii
  • A. P. Dyban


We present the data on the in vitro development of mouse embryos after injecting pronuclei with cloned DNA fragments with an enhanced green fluorescent protein (EGFP) gene under the control of different regulatory elements (promoters, enhancers, stop signals, etc). It was found that the microinjection procedure itself inhibits development independent of the genetic constructs applied. The development of transgenic embryos may be blocked at different stages of cleavage and morula or blastocyte stages. Most transgenic embryos proved to be mosaics. Transgenic cells are very frequently found in trophectoderm; i.e. they are not a part of the inner cell mass, which is a progenitor for the development of embryo tissues.


mouse embryos EGFP transgenic embryos activation of zygotic genes 



sodium dodecyl sulfate


base pairs


sodium tetraacetic acid


enhanced green fluorescence protein




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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • A. V. Sorokin
    • 1
  • E. M. Nonyashvili
    • 1
  • O. V. Kidgotko
    • 1
  • L. K. Sasina
    • 1
  • T. D. Aleinikova
    • 1
  • M. M. Shavlovskii
    • 1
  • S. I. Gorodetskii
    • 2
  • A. P. Dyban
    • 1
  1. 1.Institute of Experimental MedicineRussian Academy of Medical SciencesSt. PetersburgRussia
  2. 2.Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia

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