Cell and Tissue Biology

, 2:93 | Cite as

Micronucleus and antitumor activities of two newly synthesized pyrimidine derivatives

  • A. K. Nersesyan
  • R. E. Muradyan
  • F. G. Arsenyan
  • G. G. Danagulyan


Micronucleus and antitumor activities of two newly synthesized iodides of pyrazolo[1,5a]pyrimidines were studied on mice. Both compounds were slightly toxic and had no antitumor activity in mice with Ehrlich ascites carcinoma; only one of them had low micronucleus activity. Both compounds substantially increase the micronucleus as well as the antitumor activities of cyclophosphamide.

Key words

pyrazolo[1,5a] pyrimidine iodides mice micronuclei Ehrlich ascitic carcinoma antitumor activity cyclophosphamide 


  1. Castro Kreder, N., Van Bree, C., Franken, N.A., and Havemann, J., Effects of Gemcitabine on Cell Survival and Chromosome Aberrations after Pulsed Low Dose-rate Irradiation, J. Radiat. Res. (Tokyo), 2004, vol. 45, pp. 111–118.CrossRefGoogle Scholar
  2. Castro Kreder, N., Van Bree, C., Franken, N.A., Havemann, J., Jagetia, G.C., and Rao, S.K., Evaluation of the Antineoplastic Activity of Guduchi (Tinospora cordifolia) in Ehrlich Ascites Carcinoma Bearing Mice, Biol. Pharm. Bull., 2006, vol. 29, pp. 460–466.CrossRefGoogle Scholar
  3. CSGMT/JEMS.MMS, Single versus Multiple Dosing in the Micronucleus Test: the Summary of the Fourth Collaborative Study by CSGMT/JEMS.MMS. Collaborative Study Group for the Micronucleus Test, the Mammalian Mutagenesis Study Group of the Environmental Mutagen Society, Japan (CSGMT/JEMS.MMS), Mutat. Res., 1990, vol. 234, pp. 205–222.Google Scholar
  4. Danagulyan, G.G., Boyakhchyan, A.P., Sahakyan, L.G., and Danagulyan, A.G., Proceed. Intern. Conf. on Heterocycl. Comp. Devoted to the 90-th Annivers. A.N. Kost, Moscow, 2005, pp. 157–158.Google Scholar
  5. Izmerov, N.F., Sanotskii, I.V., and Sidorov, K.K. Parametry toksimetrii promyshlennykh yadov pri odnokratnom vozdeistvii (Parameters of Toximetry of Industrial Poisons at Single Action), Moscow: Meditsina, 1977, 256 pp.Google Scholar
  6. Jagetia, G.C. and Rao, S.K. Evaluation of the Antineoplastic Activity of Guduchi (Tinospora cordifolia) in Ehrlich Ascites Carcinoma Bearing Mice, Biol. Pharm. Bull., 2006, vol. 29, pp. 460–466.CrossRefPubMedGoogle Scholar
  7. Junior, A.D., Mota, L.G., Nunan, E.A., Wainstein, A.J., Wainstein, A.P., Leal, A.S., Cardoso, V.N., and De Oliveira, M.C., Tissue Distribution Evaluation of Stealth pHsensitive Liposomal Cisplatin versus Free Cisplatin in Ehrlich Tumor-Bearing Mice, Life Sci., 2007, vol. 80, pp. 659–664.CrossRefPubMedGoogle Scholar
  8. Kirkhart, B., Micronucleus Test on 21 Compounds. In: Evaluation of Short-term Tests for Carcinogenens, Geneva: University Press, 1981, vol. 1, pp. 698–704.Google Scholar
  9. Lakshmi, C., Deb, C., Ray, C., and Ray, M.R., Reduction of Hematotoxicity and Augmentation of Antitumor Efficacy of Cyclophosphamide by Dopamine, Neoplasma, 2005, vol. 52, pp. 68–73.PubMedGoogle Scholar
  10. Marzano, C., Bettio, F., Baccichetti, F., Trevisan, A., Giovagnini, L., and Fregona, D., Antitumor Activity of a New Platinum(II) Complex with Low Nephrotoxicity and Genotoxicity, Chem. Biol. Interact., 2004, vol. 148, pp. 37–48.CrossRefPubMedGoogle Scholar
  11. Nersesyan, A. and Muradyan, R., Sea-buckthorn Juice Protects Mice against Genotoxic Action of Cisplatin, Exp. Oncol., 2004, vol. 26, pp. 153–155.PubMedGoogle Scholar
  12. Nersesyan, A., Muradyan, R., Arsenyan, F., and Danagulyan, G., Micronucleus-inducing and Antitumor Activity of Three Newly Synthesized Bridged Nitrogen Atom-containing Pyrimidines, J. BUON, 2006, vol. 11, pp. 329–334.PubMedGoogle Scholar
  13. Oncology Prescribing Guide, Glaxo Smith Kline, Tallahassee, FL, USA, 2001.Google Scholar
  14. Papageorgiou, A., Nikolaropoulos, S.S., Arsenou, E.S., Karaberis, E., Mourelatos, D., Kotsis A., and Chryssogelou, E., Enhanced Cytogenetic and Antineoplastic Effects by the Combined Action of Two Esteric Steroidal Derivatives of Nitrogen Mustards, Chemother., 1999, vol. 45, pp. 61–67.CrossRefGoogle Scholar
  15. Quintana, E., Pertusa, J., Gonzalez, R., and Renau-Piqueras, J., Carboplatin Treatment Induces Dose-dependent Increases in the Frequency of Micronuclei in Ehrlich Ascites Tumor Cells, Mutat. Res., 1994, vol. 322, pp. 55–60.CrossRefPubMedGoogle Scholar
  16. Rathinasamy, S., Karki, S.S., Bhattacharya, S., Manikandan, L., Prabakaran, S.G., Gupta, M., and Mazumder, U.K., Synthesis and Anticancer Activity of Certain Mononuclear Ru (II) Complexes, J. Enzyme Inhib. Med. Chem., 2006, vol. 21, pp. 501–507.CrossRefPubMedGoogle Scholar
  17. Valovicova, Z. and Gabelova, A., Effect of Cytosine Arabinoside and Hydroxyurea on Micronucleus Formation Induced by Model Clastogens in Chinese Hamster V79 Cells, Neoplasma, 2004, vol. 51, pp. 442–449.PubMedGoogle Scholar

Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • A. K. Nersesyan
    • 1
  • R. E. Muradyan
    • 2
  • F. G. Arsenyan
    • 2
  • G. G. Danagulyan
    • 3
  1. 1.National Center of OncologyYerevanArmenia
  2. 2.Institute of Fine Organic ChemistryYerevanArmenia
  3. 3.Institute of Organic ChemistryYerevanArmenia

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