Effects of hydrogen peroxide on neutrophil ability to generate reactive oxygen and chlorine species and to secrete myeloperoxidase in vitro
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In this work, the effects of H2O2 at concentrations of 10−8–10−2 mol/l on the neutrophil ability to generate reactive oxygen and chlorine species (ROCS) and to secrete myeloperoxidase (MPO) were studied, as well as the H2O2 damaging action on neutrophils. It was found that H2O2 at concentrations of 2 × 10−3–10−2 mol/l led to disturbances of neutrophil membrane barrier properties and to a lactate dehydrogenase release. Incubation of neutrophils with an addition of 10−4–10−7 mol/l H2O2 was accompanied by an increase of the cell ability to generate ROCS during phagocytosis and a decrease of neutrophil ability to secrete MPO and ROCS into the extracellular medium during adhesion. Mechanisms of the H2O2 action are coupled with arachidonic acid metabolism. Inhibition of the 5-lipoxygenase or cyclooxygenase metabolism pathways produced an enhancement of the H2O2 destructive effect. Block of 5-lipoxygenase pathway led to elimination of the H2O2 action on MPO and ROCS secretion and to an enhancement of the H2O2 effect on the neutrophil ability to generate ROCS during phagocytosis. The obtained data indicate a high blood neutrophil resistance to the H2O2 destructive action and confirm the H2O2 regulatory role with respect to the neutrophil functions.
Key wordsneutrophils hydrogen peroxide reactive oxygen and chlorine species myeloperoxidase 5-lipoxygenase cyclooxygenase lactate dehydrogenase
reactive oxygen and chlorine species
balanced buffered salt solution
- rel. un.
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