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Cell and Tissue Biology

, Volume 1, Issue 4, pp 277–292 | Cite as

The dynamics and mechanisms of nucleolar reorganization during mitosis

Article

Abstract

In the vast majority of eukaryotic somatic cells the nucleolus is the largest and the most dynamic nuclear domain. The canonical nucleolar function is ribosome biogenesis, which includes ribosomal DNA (rDNA) transcription, pre-rRNA processing and ribosomal subunit assembly. Furthermore, according to recent data the nucleolus and specific nucleolar proteins participate in cell cycle regulation, apoptosis and control of aging. These functions are mostly realized by the nucleolus at interphase and become downregulated during mitosis, when the nucleolus disassembles.

This review summarizes the current data on the dynamics and mechanisms of the disassembly and reassembly of the nucleolus during mitosis. Particular attention is given to the information obtained by analysis of the dynamics of the nucleolus in living cells and by modeling of the premature assembly of the nucleolus under various experimental conditions.

Key words

nucleolus mitosis mechanisms of disassembly and reassembly peripheral chromosomal material nucleolus-derived foci prenucleolar bodies analytic methods 

Abbreviations

GC

granular component

snoRNA

small nucleolar RNA

pre-RNA

47-45S rRNA precursor

DFC

dense fibrillar component

PCM

peripheral chromosome material

rDNA

ribosomal DNA

rRNA

ribosomal RNA

FC

fibrillar center

NDF

nucleolus-derived focus

NO

chromosomes-nucleolar organizing chromosomes

NOR

nucleolar organizing region

APC/C

anaphase promoting complex (cyclosome)

Cdk1

cyclin B-dependent kinase 1

GFP

green fluorescent protein

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© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  1. 1.A.N. Belozersky Institute of Physical and Chemical BiologyMoscow State UniversityRussia
  2. 2.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RASMoscowRussia

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