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Variability of microsatellites BM224 and Bcal7 in populations of green toads (Bufo viridis complex) differing by nuclear DNA content and ploidy

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Abstract

The variability of microsatellites BM224 and Bcal7 was studied for the first time in three species of the diploid-polyploid complex of Bufo viridis (B. viridis, B. oblongus, and B. pewzowi). The locus Bcal7 was established to be monomorphic in all samples studied. In microsatellite BM224, three allele variants were found. Among tetraploid toads, the western Asiatic species B. oblongus was characterized by one allele only, the eastern B. pewzowi, by the two other alleles. A similar distribution was also revealed in triploid individuals on the borders of range between tetraploid and diploid species. Among the diploid species B. viridis samples, all three allele variants of microsatellite BM224 were observed. Their distribution in the area proved to be geographically determined. In diploid toads, a similarity was revealed between the distribution of microsatellite BM224 alleles and variability of the nuclear DNA content.

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    S. N. Litvinchuk, Yu. M. Rozanov, N. M. Usmanova & V. I. Kazakov

  2. Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia

    L. Ya. Borkin

  3. Dagestan State University, Makhachkala, Dagestan, Russia

    L. F. Mazanaeva

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Original Russian Text © S.N. Litvinchuk, Yu.M. Rozanov, N.M. Usmanova, L.Ya. Borkin, L.F. Mazanaeva, V.I. Kazakov, 2007, published in Tsitologiya, Vol. 48, No. 4, 2007, pp. 332–345.

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Litvinchuk, S.N., Rozanov, Y.M., Usmanova, N.M. et al. Variability of microsatellites BM224 and Bcal7 in populations of green toads (Bufo viridis complex) differing by nuclear DNA content and ploidy. Cell Tiss. Biol. 1, 65–79 (2007). https://doi.org/10.1134/S1990519X07010099

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