Neurochemical Journal

, Volume 12, Issue 1, pp 33–40 | Cite as

Melatonin Modulates Phosphorylation of 2′,3′-Cyclic Nucleotide-3′-Phosphodiesterase in the Presence of Protoporphyrin IX in the Brain Mitochondria of Rats during the Functioning of the Non-Specific Mitochondrial Pore

  • O. V. Krestinina
  • Yu. L. Baburina
  • I. V. Odinokova
  • T. S. Azarashvili
  • V. S. Akatov
Experimental Articles


Melatonin (N-acetyl-5-methoxytryptamine), a neuroendocrine hormone of the pineal gland, participates in the modulation of the mitochondrial nonspecific pore (mitochondrial permeability transition pore, mPTP). According to the results we obtained, protoporphyrin IX (PPIX, a ligand of the TSPO translocator protein) induces the opening of the mPTP in brain mitochondria; melatonin slows the induction of the mPTP in rat brain mitochondria incubated with PPIX. Induction of the mPTP activates protein kinases/protein phosphatases that are involved in the regulation of protein phosphorylation. The TSPO modulates protein phosphorylation; in the presence of PPIX, the phosphorylation of 2′,3′-cyclic nucleotide-3′-phosphodiesterase (CNPase) increases. Here, we showed an increase in the degree of phosphorylation of CNPase in PPIX-treated rat brain mitochondria, a melatonin-induced decrease in level of CNPase phosphorylation in mitochondria incubated with PPIX; hence, we propose that melatonin participates in TSPOmodulated protein phosphorylation.


melatonin rat brain mitochondria protein phosphorylation protoporphyrin IX non-specific permeability pore 2′,3′-cyclic nucleotide-3′-phosphodiesterase (CNPase) 


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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • O. V. Krestinina
    • 1
    • 2
  • Yu. L. Baburina
    • 1
  • I. V. Odinokova
    • 1
  • T. S. Azarashvili
    • 1
  • V. S. Akatov
    • 1
  1. 1.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesPushchinoRussia
  2. 2.Pushchino, Moscow oblastRussia

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