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Neurochemical Journal

, Volume 8, Issue 3, pp 221–225 | Cite as

Phosphorylated neurofilament heavy subunits as a marker of neurodegeneration in demyelinating diseases of the CNS

  • A. A. Vorobyeva
  • V. V. Fominykh
  • M. V. Onufriev
  • M. N. Zakharova
  • N. V. Gulyaeva
Clinical Neurochemistry
  • 67 Downloads

Abstract

Neurofilaments are used as biomarkers of neurodegeneration, i.e., neuroinflammatory destruction of neurons, in various diseases of the nervous system. The purpose of our study is to clarify data on the pathogenesis and clinical prognosis of chronic and rare monophase demyelinating diseases. The content of phosphorylated neurofilament heavy subunits (pNFH) was measured in the cerebrospinal fluid (CSF) and blood serum using ELISA. The following groups of patients were studied: remittent multiple sclerosis (RMS, n = 56), secondary progressive multiple sclerosis (SPMS, n = 20), acute disseminated encephalomyelitis (ADEM, n = 7), neuromyelitis optica (NMO, n = 5), amyotrophic lateral sclerosis (ALS, n = 26), and the control group (n = 26). We found significant differences in the content of pNFH in the CSF between the RMS, ADEM, ALS groups and the control group; however, we did not observe any differences between the RMS, ADEM, and ALS groups per se. We did not find any differences in the contents of pNFH in the blood serum or any correlations of the pNFH levels with clinical features of diseases in these groups of patients. Based on this index, we conclude that neurodegeneration occurs in RMS, ADEM, and ALS. The pNFH content should be studied in larger cohorts of patients and at strictly defined time points.

Keywords

neurofilaments neurodegeneration demyelinating diseases multiple sclerosis cerebrospinal fluid blood serum 

Abbreviations

ALS

amyotrophic lateral sclerosis

SPMS

secondary progressive multiple sclerosis

NF

neurofilament sub-units

NMO

neuromyelitis optica

ADEM

acute disseminated encephalomyelitis

RMS

remittent multiple sclerosis

CSF

cerebrospinal fluid

pNFM

phosphorylated neurofilament moderate subunits

NFL

neurofilament light subunits

pNFH

phosphorylated neurofilament heavy subunits

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • A. A. Vorobyeva
    • 2
    • 3
  • V. V. Fominykh
    • 1
    • 2
  • M. V. Onufriev
    • 1
  • M. N. Zakharova
    • 2
  • N. V. Gulyaeva
    • 1
  1. 1.Institute of Higher Nervous Activity and NeurophysiologyRussian Academy of SciencesMoscowRussia
  2. 2.Research Center of NeurologyRussian Academy of Medical SciencesMoscowRussia
  3. 3.MoscowRussia

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