Neurochemical Journal

, Volume 5, Issue 1, pp 52–68 | Cite as

Analysis of dynamics of degenerative and regenerative processes in the flexor and extensor collaterals of crushed sciatic nerve: Effects of parathyroid hormone

  • A. L. Minasyan
  • A. V. Aznauryan
  • I. B. Meliksetyan
  • V. A. Chavushyan
  • J. S. Sarkissian
Experimental Articles

Abstract

We performed morphological, histochemical, and electrophysiological analysis of changes in the state of the rat sciatic nerve after nerve crushing. The activity of Ca2+-dependent acidic phosphatase (AP) was measured in sections of the nerve fibers to trace the recovery of the nerve after its injury. The systemic application of parathyroid hormone (PTH) resulted in the acceleration of injured nerve recovery. Proliferation of both endoneurium and Schwann cells was observed on the 3rd day after the crushing of the ipsilateral extensor (N. peroneus communis) and flexor (N. gastrocnemius) nerve bundles. A complete recovery of the AP enzymatic activity was found on the 7–9th days after the crushing. From the 5th to 35th days after the crushing, we performed online registration and mathematical analysis of pulse activity. In the control experiments, from the 35th to 70th days, high-frequency stimulation of the nerves showed the regeneration of the extensor nerve only accompanied by alterations in fiber direction. In the presence of PTH, the regeneration was significantly accelerated and it was associated with early regeneration of the flexor nerve and more structured fiber morphology. We found a correlation between the electrophysiological and morphological data.

Keywords

nerve crushing sciatic nerve motoneurons spinal cord high frequency stimulation extensor and flexor collaterals parathyroid hormone 

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References

  1. 1.
    Minasyan, A.L., Aznavuryan, A.V., Chavushyan, V.A., and Sarkisyan, D.S., Vopr. Teoret. i Klin. Meditsiny. Nauchn.-Prakt. Zhurn., 2008, vol. 11, pp. 54–62.Google Scholar
  2. 2.
    Gupta, R. and Steward, O., J. Comp. Neurol., 2003, vol. 461, no. 2, pp. 174–186.PubMedCrossRefGoogle Scholar
  3. 3.
    Stoll, G. and Müller, H.W., Brain Pathol., 1999, vol. 9, no. 2, pp. 313–325.PubMedCrossRefGoogle Scholar
  4. 4.
    George, L.T., Myckatyn, T.M., Jensen, J.N., Hunter, D.A., and Mackinnon, S.E., J. Reconstr. Microsurg., 2003, vol. 19, no. 1, pp. 41–48.PubMedCrossRefGoogle Scholar
  5. 5.
    Reyesarmijo, E., Rev. Med. Hosp. Gen. (mex), 1964, vol. 27, pp. 107–114.Google Scholar
  6. 6.
    Aydin, M.A., Comlekci, S., Ozguner, M., Cesur, G., Nasir, S., and Aydin, Z.D., Bioelectromagnetics, 2006, vol. 27, no. 5, pp. 401–413.PubMedCrossRefGoogle Scholar
  7. 7.
    Bervar, M., Bioelectromagnetics, 2005, vol. 26, no. 5, pp. 351–356.PubMedCrossRefGoogle Scholar
  8. 8.
    Fargo, K.N., Alexander, T.D., Tanzer, L., Poletti, A., and Jones, K.J., J. Neurotrauma, 2008, vol. 25, no. 5, pp. 561–566.PubMedCrossRefGoogle Scholar
  9. 9.
    Fleming, C.E., Saraiva, M.J., and Sousa, M.M., J. Neurochem., 2007, vol. 103, no. 2, pp. 831–839.PubMedCrossRefGoogle Scholar
  10. 10.
    Kato, N., Nemoto, K., Nakanishi, K., Morishita, R., Kaneda, Y., Uenoyama, M., Ikeda, T., Fujikawa, K., and Nonviral, H., Neurosci. Res., 2005, vol. 52, no. 4, pp. 299–310.PubMedCrossRefGoogle Scholar
  11. 11.
    Mohri, T., Tanaka, H., Tajima, G., et al., Shock, 2006, vol. 26, no. 6, pp. 581–586.PubMedCrossRefGoogle Scholar
  12. 12.
    Macica, C.M., Liang, G., and Lankford, K.L., Glia, 2006, vol. 53, no. 6, pp. 637–648.PubMedCrossRefGoogle Scholar
  13. 13.
    Paxinos, G. and Watson, C., The Rat Brain in Stereotaxic Coordinates. 5th Ed, New York: Academic Press, 2005.Google Scholar
  14. 14.
    Minasyan, A.L., Aznauryan, A.V., Chavushyan, V.A., and Sarkissian, J.S., New Armenian Med. J., 2009, vol. 3, no. 1, pp. 44–58.Google Scholar
  15. 15.
    Meliksetyan, I.B., Morfologiya (St. Petersburg), vol. 131, no. 2, pp. 77–80.Google Scholar
  16. 16.
    de Ruiter, G.C., Malessy, M.J., Alaid, A.O., Spinner, R.J., Engelstad, J.K., Sorenson, E.J., Kaufman, K.R., Dyck, P.J., and Windebank, A.J., Exp. Neurol., 2008, vol. 211, no. 2, pp. 339–350.PubMedCrossRefGoogle Scholar
  17. 17.
    Fugleholm, K., Schmalbruch, H., and Krarup, C., J. Peripher. Nervst., 2000, vol. 5, no. 2, pp. 82–95.CrossRefGoogle Scholar
  18. 18.
    Foehring, R.C., Sypert, G.W., and Munson, J.B., J. Neurophysiol., 1986, vol. 55, no. 5, pp. 931–946.PubMedGoogle Scholar
  19. 19.
    Foehring, R.C., Sypert, G.W., and Munson, J.B., J. Neurophysiol., 1986, vol. 55, no. 5, pp. 947–965.PubMedGoogle Scholar
  20. 20.
    Omura, T., Sano, M., Omura, K., Hasegawa, T., Doi, M., Sawada, T., and Nagano, A., Int. J. Neurosci., 2004, vol. 114, no. 12, pp. 1561–1572.PubMedCrossRefGoogle Scholar
  21. 21.
    Pola, R., Aprahamian, T.R., Bosch-Marc, M., Curry, C., Gaetani, E., Flex, A., Smith, R.C., Isner, J.M., and Losordo, D.W., Neurobiol. Aging, 2004, vol. 25, no. 10, pp. 1361–1368.PubMedCrossRefGoogle Scholar
  22. 22.
    Moldovan, M., Sørensen, J., and Krarup, C., Brain, 2006, vol. 129, pp. 2471–2483.PubMedCrossRefGoogle Scholar
  23. 23.
    Cuppini, R., Ambrogini, P., Sartini, S., Bruno, C., Lattanzi, D., and Rocchi, M.B., Somatosens. Mot. Res., 2002, vol. 19, no. 4, pp. 279–285.PubMedCrossRefGoogle Scholar
  24. 24.
    Palop, J.J., Chin, J., Roberson, E.D., Wang, J., Thwin, M.T., Bien-Ly Nga, Yoo Jong, O. Kaitlyn H., Yu Gui-Qiu, Kreitzer A., Finkbeiner S., Noebels J.L., and Mucke L., Neuron, 2007, vol. 55, pp. 697–711.PubMedCrossRefGoogle Scholar
  25. 25.
    Owens, D.F. and Kriegstein, A.R., Nat. Rev. Neurosci., 2002, vol. 3, pp. 715–727.PubMedCrossRefGoogle Scholar
  26. 26.
    Tighilet, B. and Lacour, M., Eur. J. Neurosci., 2001, vol. 13, pp. 2255–2267.PubMedCrossRefGoogle Scholar
  27. 27.
    Giardino, L., Zanni, M., Fernandez, M., Battaglia, A., Pignataro, O., and Calza’ L., Brain Res., 2002, vol. 929, no. 1, pp. 76–86.PubMedCrossRefGoogle Scholar
  28. 28.
    Johnston, A.R., Him, A., and Dutia, M.B., Neuroreport., 2001, vol. 12, pp. 597–600.PubMedCrossRefGoogle Scholar
  29. 29.
    Tighilet, B. and Lacour, M., Eur. J. Neurosci., 2001, vol. 13, pp. 2255–2267.PubMedCrossRefGoogle Scholar
  30. 30.
    Yamanaka, T., Him, A., Cameron, S.A., and Dutia, M.B., J. Physiol., 2000, vol. 523, pp. 413–424.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • A. L. Minasyan
    • 1
  • A. V. Aznauryan
    • 1
  • I. B. Meliksetyan
    • 2
  • V. A. Chavushyan
    • 2
  • J. S. Sarkissian
    • 2
  1. 1.Heratsi Yerevan State Medical UniversityYerevanArmenia
  2. 2.Orbeli Institute of PhysiologyNational Academy of SciencesYerevanArmenia

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