Advertisement

Neurochemical Journal

, Volume 3, Issue 2, pp 139–144 | Cite as

Crosslinkers and their utilization for studies of intermolecular interactions

Methodical Articles

Abstract

We analyzed a new direction of studies of intermolecular interactions in cells with the use of crosslinkers and the possible utilization of crosslinkers in neurochemical studies. We reviewed current crosslinkers and described their main physicochemical characteristics. We also described the main specific features of utilization of crosslinkers in biological studies and, especially, in studies of the brain. We presented the general scheme of a typical experiment.

Key words

brain enzymes substrates crosslinkers 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Conrad, K.L., Tseng, K.Y., Uejima, J.L., et al., Nature, 2008, vol. 454, no. 7200, pp. 118–121.PubMedCrossRefGoogle Scholar
  2. 2.
    Petrotchenko, E.V., Olkhovik, V.K., and Borchers, C.H., Mol. Cell. Proteomics, 2005, vol. 4, no. 8, pp. 1167–1179.PubMedCrossRefGoogle Scholar
  3. 3.
    Fujita, N. and Wade, P.A., Methods, 2004, vol. 33, no. 1, pp. 81–85.PubMedCrossRefGoogle Scholar
  4. 4.
    Schneider, C., Newman, R.A., Sutherland, D.R., et al., J. Biol. Chem., 1982, vol. 257, no. 18, pp. 10766–10769.PubMedGoogle Scholar
  5. 5.
    Grabarek, Z. and Gergely, J., Anal. Biochem., 1990, vol. 185, no. 1, pp. 131–135.PubMedCrossRefGoogle Scholar
  6. 6.
    Orlando, V., Strutt, H., and Paro, R., Methods, 1997, vol. 11, no. 2, pp. 205–214.PubMedCrossRefGoogle Scholar
  7. 7.
    Schmitt-Ulms, G., Hansen, K., Liu, J., et al., Nature Biotechnol, 2004, vol. 22, no. 6, pp. 724–731.CrossRefGoogle Scholar
  8. 8.
    Taggart, A.K. and Pugh, B.F., Science, 1996, vol. 272, no. 5266, pp. 1331–1333.PubMedCrossRefGoogle Scholar
  9. 9.
    Tanaka, Y. and Kohler, J.J., J. Am. Chem. Soc., 2008, vol. 130, no. 11, pp. 3278–3279.PubMedCrossRefGoogle Scholar
  10. 10.
    Suchanek, M., Radzikowska, A., and Thiele, C., Nature Methods, 2005, vol. 2, no. 4, pp. 261–267.PubMedCrossRefGoogle Scholar

Copyright information

© MAIK Nauka 2009

Authors and Affiliations

  1. 1.Institute of Higher Nervous Activity and NeurophysiologyRussian Academy of SciencesMoscowRussia
  2. 2.MoscowRussia

Personalised recommendations