Skip to main content
SpringerLink
Log in

Role of the cAMP cascade in the turnover of synaptic vesicles of the frog motor nerve terminal

  • Experimental Articles
  • Published:
Neurochemical Journal Aims and scope Submit manuscript

Abstract

In experiments with frog neuromuscular preparations we have shown with the use of electrophysiological (two-electrode voltage clamp) and optical (fluorescent exocytotic dye FM1-43) techniques that during high-frequency stimulation (20 imp/s, 3 min) the cyclic adenosine monophosphate (cAMP) system had a complex effect on the exo-endocytotic cycle of synaptic vesicles. The activation of cAMP-dependent enzymes (100 μM 8-Br-cAMP, 50 μM Bt2-cAMP) was accompanied by facilitation of exocytosis of the vesicles from a ready-to-release pool and enhancement of the endocytosis of synaptic vesicles. However, transport of the vesicles from the mobilization pool to the ready-to-release pool was disturbed and transmitter release was supported by the vesicles from the reserve pool. Blockage of adenylate cyclase (1 μM MDL) suppressed exocytosis of the vesicles from the ready-to-release pool, hindered replenishment of this pool with vesicles from the mobilization and reserve pools, and impaired endocytosis. Thus, stimulation of the cAMP pathway promotes vesicle recycling via a slow pathway and maintenance of transmitter release during high-frequency activity via vesicles from the reserve pool, whereas the background activity of adenylate cyclase is necessary for the effective development of all the key stages of the vesicular cycle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Heuser, J.E., Reese, T.S., Dennis, M.J., and Jan, L., J. Cell Biol., 1979, vol. 81, pp. 275–300.

    Article  PubMed  CAS  Google Scholar 

  2. Sudhof, T.C., Annu. Rev. Neurosci., 2004, vol. 27, pp.509–547.

    Article  PubMed  Google Scholar 

  3. Zhai, R.G. and Bellen, H.J., J. Physiol., 2004, vol. 19, pp. 262–270.

    Article  Google Scholar 

  4. Gundelfinger, E.M., Kessels, M.M., and Qualmann, B., Nature Rev. Mol. Cell Biol., 2003, vol. 4, pp. 127–139.

    Article  CAS  Google Scholar 

  5. Betz, W.J. and Angelson, J.K., Annu. Rev. Physiol., 1998, vol. 60, pp. 347–363.

    Article  PubMed  CAS  Google Scholar 

  6. Kavalali, E. T., Neuroscientist, 2006, vol. 12, no. 1, pp. 57–66.

    Article  PubMed  CAS  Google Scholar 

  7. Micheva, K.D., Buchanana, J., Holz, R.W., and Smith, S.J., Nature Neurosci., 2003, vol. 6, no. 9, pp. 925–932.

    Article  PubMed  CAS  Google Scholar 

  8. Rizzoli, S.O. and Betz, W.J., Nature Rev. Neurosci., 2005, vol. 6, pp. 57–69.

    Article  CAS  Google Scholar 

  9. Richards, D.A., Guatimosim, C., and Betz, W.J., Neuron, 2000, vol. 27, pp. 551–559.

    Article  PubMed  CAS  Google Scholar 

  10. Rohrbough, J. and Broadie, K., Nature Rev. Neurosci., 2005, vol. 6, pp. 139–150.

    Article  CAS  Google Scholar 

  11. Wenk, M.R. and De Camilli, P., Proc. Nat. Acad. Sci., 2004, vol. 101, no. 6, pp. 8262–8269.

    Article  PubMed  CAS  Google Scholar 

  12. Seino, S. and Shibasaki, T., Physiol. Rev., 2005, vol. 85, pp. 1303–1342.

    Article  PubMed  CAS  Google Scholar 

  13. Verstreken, P., Ly, C., Venken, K.J.T., et al., Neuron, 2005, vol. 47, pp. 365–378.

    Article  PubMed  CAS  Google Scholar 

  14. Zefirov, A.L., Grigor’ev, P.N., Petrov, A.M., et al., Tsitologiya, 2003, vol. 45, no. 12, pp. 1163–1171.

    CAS  Google Scholar 

  15. Zefirov A.L., Abdrakhmanov, M.M., Grigor’ev, P.N., and Petrov, A.M., Tsitologiya, 2006, vol. 48, no. 1, pp. 34–41.

    CAS  Google Scholar 

  16. Betz, W.J. and Bewick, G.S., J. Physiol. Lond., 1993, vol. 460, pp. 287–309.

    PubMed  CAS  Google Scholar 

  17. Reid, B., Slater, C.R., and Bewick, G.S., J. Neurosci., 1999, vol. 19, no. 7, pp. 2511–2521.

    PubMed  CAS  Google Scholar 

  18. Hepp, R., Cabaniols, J.P., and Roche, P.A., FEBS Lett., 2002, vol. 532, pp. 52–56.

    Article  PubMed  CAS  Google Scholar 

  19. Nagy, G., Reim, K., Matti, U., et al., Neuron, 2004, vol. 41, pp. 351–365.

    Article  Google Scholar 

  20. Evans, G.J. and Morgan, A., J. Biochem., 2002, vol. 364, pp. 343–347.

    Article  CAS  Google Scholar 

  21. Chheda, M.G., Ashery, U., Thakur, P., et al., Nat. Cell Biol., 2001, vol. 3, pp. 331–338.

    Article  PubMed  CAS  Google Scholar 

  22. Barder, M.-F., Dousssau, F., Chasserot-Golaz, S., et al., Biochim. Biophys. Acta., 2004, vol. 1742. pp. 37–49.

    Article  Google Scholar 

  23. Wang, Y., Chen, Y., Chen, M., and Xu, W., Oncol. Reports, 2006, vol. 16. pp. 755–761.

    CAS  Google Scholar 

  24. Gaffield, M.A., Rizzoli, S.O., and Betz, W.J., Neuron, 2006, vol. 51, pp. 317–325.

    Article  PubMed  CAS  Google Scholar 

  25. Kuromi, H. and Kidokoro, Y., Neuron, 2002, vol. 35, pp. 333–343.

    Article  PubMed  CAS  Google Scholar 

  26. Jovanovic, J.N, Sihra, T.S., Narin, A.C., et al., J. Neurosci., 2001, vol. 21. pp. 7944–7953.

    PubMed  CAS  Google Scholar 

  27. Richards, D.A, Rizzoli, S.O., and Betz, W.J., J. Physiol., 2004, vol. 557, no. 1, pp. 77–91.

    Article  PubMed  CAS  Google Scholar 

  28. Tasken, K. and Aandahl, E.M., Physiol. Rev., 2004, vol. 84, pp. 137–167.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kazan State University, Kazan, Tatarstan, Russia

    A. M. Petrov, A. R. Giniatullin & A. L. Zefirov

Authors
  1. A. M. Petrov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. R. Giniatullin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. L. Zefirov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. M. Petrov.

Additional information

Original Russian Text © A.M. Petrov, A.R. Giniatullin, A.L. Zefirov, 2008, published in Neirokhimiya, 2008, Vol. 25, No. 3, pp. 202–210.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Petrov, A.M., Giniatullin, A.R. & Zefirov, A.L. Role of the cAMP cascade in the turnover of synaptic vesicles of the frog motor nerve terminal. Neurochem. J. 2, 175–182 (2008). https://doi.org/10.1134/S1819712408030069

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1819712408030069

Key words

Search

Navigation