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Neurochemical Journal

, Volume 1, Issue 3, pp 196–203 | Cite as

Chaperone-like activity of immunophilin FKBP12 from bovine brain, a cytoplasmic receptor of immunosuppressor FK506

Experimental Articles

Abstract

The chaperone-like activity of brain immunophilin—a cytoplasmic receptor of immunosuppressor FK506 (FK506-binding protein, FKBP12) was evaluated in a test-system in vitro based on suppression of insulin aggregation as a result of A-and B-chain dissociation induced by disulfide bond reduction by dithiotreitol. Using dynamic light scattering technique we have demonstrated the concentration dependent suppression of light scattering intensity and the decrease of the hydrodynamic radius of insulin B-chain amorphous aggregates upon addition of FKBP12 to the incubation medium, a significant effect being revealed even in the substoichiometric concentration range. The results provide evidence that FKBP12 possesses chaperone-like activity preventing insulin aggregation.

Key words

protein aggregation chaperones insulin neurodegeneration immunophilin brain 

Abbreviations

β-ME

β-mercaptoethanol

DLS

dynamic light scattering

FKBP12

FK506-binding protein

PDI

protein disulfide isomerase

PMSF

phenylmethylsulfonyl fluoride

PPI

peptidyl-prolyl cis-trans-isomerase

SDS

sodium dodecyl sulfate

TFA

trifluoroacetic acid

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Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  • E. M. Lyutova
    • 1
  • A. S. Kasakov
    • 1
  • B. Ya. Gurvits
    • 1
  1. 1.Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia

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