Doklady Biochemistry and Biophysics

, Volume 485, Issue 1, pp 150–152 | Cite as

Xenobiotic Response Elements (XREs) from Human CYP1A1 Gene Enhance the hTERT Promoter Activity

  • M. V. ShepelevEmail author
  • S. V. Kalinichenko
  • E. K. Saakian
  • I. V. Korobko


A hybrid 6XRE–hTERT promoter consisting of the hTERT tumor-specific promoter and six copies of the XRE element from the CYP1A1 human gene promoter was created. Using a human lung cancer cells as a model, we showed that XRE elements in the hybrid promoter greatly increase the activity of the hTERT promoter and ensure the reporter gene transcriptional activation in response to the treatment of the cells with the AhR ligand benzo(a)pyrene. However, similar effects were also observed in normal human bronchial epithelial cells HBEpC, which indicates the loss of the tumor-specific activity by the 6XRE–hTERT hybrid promoter. XRE elements can be used for nonspecific transcription enhancement but are unsuitable for the creation of tumor-specific promoters with enhanced activity.



  1. 1.
    Das, S.K., Menezes, M.E., Bhatia, S., Wang, X.Y., Emdad, L., Sarkar, D., and Fisher, P.B., J. Cell Physiol., 2015, vol. 230, pp. 259–271.CrossRefGoogle Scholar
  2. 2.
    Fujiwara, T., Urata, Y., and Tanaka, N., Curr. Cancer Drug Targets, 2007, vol. 7, pp. 191–201.CrossRefGoogle Scholar
  3. 3.
    Wirth, T., Zender, L., Schulte, B., Mund, B., Plentz, R., Rudolph, K.L., Manns, M., Kubicka, S., and Kuhnel, F., Cancer Res., 2003, vol. 63, pp. 3181–3188.Google Scholar
  4. 4.
    Davis, J.J., Wang, L., Dong, F., Zhang, L., Guo, W., Teraishi, F., Xu, K., Ji, L., and Fang, B., Cancer Gene Ther., 2006, vol. 13, pp. 720–723.CrossRefGoogle Scholar
  5. 5.
    Shepelev, M.V., Kopantzev, E.P., Vinogradova, T.V., Sverdlov, E.D., and Korobko, I.V., Oncol. Lett., 2016, vol. 12, pp. 1204–1210.CrossRefGoogle Scholar
  6. 6.
    Kalinichenko, S.V., Shepelev, M.V., Vikhreva, P.N., and Korobko, I.V., Acta Naturae, 2017, vol. 9, pp. 66–73.CrossRefGoogle Scholar
  7. 7.
    Murray, I.A., Patterson, A.D., and Perdew, G.H., Nat. Rev. Cancer, 2014, vol. 14, pp. 801–814.CrossRefGoogle Scholar
  8. 8.
    Li, S., Pei, X., Zhang, W., Xie, H.Q., and Zhao, B., Int. J. Mol. Sci., 2014, vol. 15, pp. 6475–6487.CrossRefGoogle Scholar
  9. 9.
    Sarkar, P., Shiizaki, K., Yonemoto, J., and Sone, H., Int. J. Oncol., 2006, vol. 28, pp. 43–51.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • M. V. Shepelev
    • 1
    Email author
  • S. V. Kalinichenko
    • 1
  • E. K. Saakian
    • 1
  • I. V. Korobko
    • 1
  1. 1.Institute of Gene Biology, Russian Academy of SciencesMoscowRussia

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