Principles for Construction of Bioluminescent Enzyme Biotests for Analysis of Complex Media
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In this study, we formulated the principles of designing bioluminescent enzyme tests for assessing the quality of complex media, which consist in providing the maximum sensitivity to potentially toxic chemicals at a minimal impact of uncontaminated complex media. The developed principles served as a basis for designing a new bioluminescent method for an integrated rapid assessment of chemical safety of fruits and vegetables, which is based on using the luminous bacteria enzymes (NAD(P)H:FMN oxidoreductase and luciferase) as a test system.
- 5.Luis, G., Hernandez, C., Rubio, C., Gonzalez-Weller, D., Gutierrez, A., Revert, C., and Hardisson, A., Nutricion Hospitalaria, 2012, vol. 27, no. 5, pp. 1605–1609.Google Scholar
- 7.Xu, T., Close, D., Smartt, A., Ripp, S., and Sayler, G., Biolumin.: Fundam. Appl. Biotechnol., 2014, vol. 1, pp. 111–151.Google Scholar
- 10.Esimbekova, E.N., Kratasyuk, V.A., and Shimomura, O., Adv. Biochem. Eng. Biotechnol., 2014, vol. 144, pp. 67–109.Google Scholar